1H NMR-based metabolomics profiling of ten new races from Capsicum annuum cv. serrano produced in Mexico

Villa‐Ruano, Nemesio; Meraz, Moisés Ramírez; Méndez-Aguilar, Reinaldo; Zepeda, L. Gerardo; Álvarez-Bravo, Arturo; Pérez‐Hernández, Nury; Becerra‐Martínez, Elvia

doi:10.1016/j.foodres.2018.10.061

Cited by 31 publications

(23 citation statements)

References 24 publications

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“…A similar effect has been observed in transgenic plants of Populus deltoides overexpressing an UDP‐glucose pyrophosphorylase . The levels of sucrose decreased by about 20% in transgenic tobacco, which suggests an increase in invertase activity . The concentration of aspartate was almost two times more abundant in transgenic samples than in wild‐type samples.…”

Section: Resultsmentioning

confidence: 99%

“…The structural identification of metabolites in both wild‐type and transgenic samples of Nicotiana tabacum were obtained on the basis of one‐dimensional (1D) and two‐dimensional (2D) NMR experiments described by Villa‐Ruano et al . The chemical identification of metabolites was achieved by comparison with published reports and databases (HMDB; http://www.hmdb.ca/;BMRB; http://www.bmrb.wisc.edu/).…”

Section: Methodsmentioning

confidence: 99%

“…The 1D 1 H‐NMR spectra were obtained by using 1D nuclear Overhauser effect spectroscopy (NOESY) presaturation pulse sequencer (Bruker 1D noesypr1d). The measurements of the pulses were carried out as previously reported …”

Section: Methodsmentioning

confidence: 99%

“…Posteriorly, the NMR spectra were analysed with MestReNova software (version 6.1.0, Mestrelabs Research SL, Santiago de Compostela, Spain). The signal of water (δ 4.75–4.90 ppm) was deleted and the spectral data were normalised and reduced to integrated buckets as previously reported . For statistical analysis purposes, after the data were converted into the ASCII format, the ASCII files were imported into SIMCA‐P version 14.0 (Umetrics, Umea, Sweden) …”

Section: Methodsmentioning

confidence: 99%

“…All variables were pareto scaled to minimise noise. The quality of the model was defined in accordance with previous works on the basis of R 2 x and Q 2 values …”

Section: Methodsmentioning

confidence: 99%

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¹H‐NMR metabolomics profiling of recombinant tobacco plants holding a promoter of a sesquiterpene cyclase

Becerra‐Martínez

Pacheco‐Hernández

Lozoya‐Gloria

et al. 2020

Phytochemical Analysis

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Introduction Nicotiana tabacum is a plant model intensively used in the bio‐engineering pharmaceutical industry as a platform to produce drugs and therapeutic agents. Currently, no information regarding the non‐targeted metabolome of transgenic tobacco containing recombinant regulatory sequences is available. Objective To compare the proton nuclear magnetic resonance (1H‐NMR) metabolomics profiling of a recombinant Nicotiana tabacum strain containing a promoter of a sesquiterpene cyclase from Capsicum annuum driving GUS expression, versus wild‐type samples. Methodology The non‐targeted 1H‐NMR metabolome was obtained and processed by principal component analysis (PCA) and orthogonal projection to latent structures discriminant analysis (OPLS‐DA). The differential metabolites were quantified by quantitative NMR. Results PCA and OPLS‐DA revealed 37 metabolites including 16 discriminant compounds for transgenic samples. Ethanol (0.4 mg g‐1), the main differential compound, was exclusively detected in transgenic tobacco; however, high levels of formate (0.28 mg g‐1) and acetate (0.3 mg g‐1) were simultaneously observed in the same group of samples. Cembratriene‐4,6‐diol, an antitumour and neuroprotective compound, and capsidiol, a known phytoalexin, increased by about 30% in transgenic samples. In addition, the endogenous levels of the antioxidant caffeoylquinic acid isomers increased by 50% in comparison to those of wild‐type tobaccos. Conclusion Our results support the occurrence of metabolic differences between wild type and transgenic tobacco containing a promoter of a Capsicum sesquiterpene cyclase gene. Interestingly, the recombinant transgenic strain studied accumulated high amounts of added value compounds with biological activity.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…All variables were pareto scaled to minimise noise. The quality of the model was defined in accordance with previous works on the basis of R 2 x and Q 2 values …”

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

¹H‐NMR metabolomics profiling of recombinant tobacco plants holding a promoter of a sesquiterpene cyclase

Becerra‐Martínez

Pacheco‐Hernández

Lozoya‐Gloria

et al. 2020

Phytochemical Analysis

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Analytical Strategies for Determining Polyphenols in Foods and Biological Samples

Olmo-García

Monasterio

Bajoub

et al. 2020

Dietary Polyphenols

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Determination of seed content in red pepper powders by ¹H NMR and second‐derivative FT‐IR spectroscopy combined with statistical analyses

Choi

Hong

Kim

et al. 2022

Bulletin Korean Chem Soc

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This study focuses on discriminating the seed content in red pepper powders using 1 H NMR and second-derivative FT-IR Spectroscopy with canonical discriminant and multiple linear regression analyses. We used 165 test samples prepared by varying the seed content for spectroscopic analyses. The canonical discriminant functions derived from 21 peak variables were used to properly discriminate the red pepper powder samples based on their seed content with a 97.6% hit ratio. Additionally, we observed an average error of 7.0% while discriminating 42 blind samples. Multiple linear regression analysis was performed to directly determine the seed content of new samples without running a statistical program. The best regression model constructed with only two variables showed an average error of 6.2% when applied to the blind samples. These results verify the feasibility of using 1 H NMR and FT-IR Spectroscopy with statistical analyses to determine the seed content in red pepper powders.

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1H NMR-based metabolomics profiling of ten new races from Capsicum annuum cv. serrano produced in Mexico

Cited by 31 publications

References 24 publications

¹H‐NMR metabolomics profiling of recombinant tobacco plants holding a promoter of a sesquiterpene cyclase

¹H‐NMR metabolomics profiling of recombinant tobacco plants holding a promoter of a sesquiterpene cyclase

Analytical Strategies for Determining Polyphenols in Foods and Biological Samples

Determination of seed content in red pepper powders by ¹H NMR and second‐derivative FT‐IR spectroscopy combined with statistical analyses

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1H NMR-based metabolomics profiling of ten new races from Capsicum annuum cv. serrano produced in Mexico

Cited by 31 publications

References 24 publications

1H‐NMR metabolomics profiling of recombinant tobacco plants holding a promoter of a sesquiterpene cyclase

1H‐NMR metabolomics profiling of recombinant tobacco plants holding a promoter of a sesquiterpene cyclase

Analytical Strategies for Determining Polyphenols in Foods and Biological Samples

Determination of seed content in red pepper powders by 1H NMR and second‐derivative FT‐IR spectroscopy combined with statistical analyses

Contact Info

Product

Resources

About

¹H‐NMR metabolomics profiling of recombinant tobacco plants holding a promoter of a sesquiterpene cyclase

¹H‐NMR metabolomics profiling of recombinant tobacco plants holding a promoter of a sesquiterpene cyclase

Determination of seed content in red pepper powders by ¹H NMR and second‐derivative FT‐IR spectroscopy combined with statistical analyses