1H-NMR spectroscopy metabonomics of reactive, ovarian carcinoma and hepatocellular carcinoma ascites

Abstract

BackgroundMetabolomic profiling of human malignant effusion remain a field poorly investigated. Proton nuclear magnetic resonance (1H-NMR) spectroscopy is a rapid relatively low cost technique, and effusion is an optimal biospecimen suitable for metabonomic investigations. With this study we addressed metabolomic profiling of malignant ascitic effusion (mAE) from patients with high grade serous ovarian carcinoma (HGSOC), Hepatocellular carcinoma (HCC), and benign AEs (bAEs) from patients with reactive peritonitis.MethodsMetabolic profiling with 1H-NMR was performed on 72 AEs (31 HGSOC, 16 HCC and 25 bAE) prospectively collected in our cytology service. Histological confirmation was requested for all malignant case. Multivariate analysis comprising PCA and PLS-DA was applied to discover metabolites suitable to differentiate effusions among the investigated groups.Results1H-NMR metabonomic analysis showed clearly different spectra for malignant and benign AEs, as well as for HGSOC vs. HCC effusion. When compared with HCC effusions, the HGSOC effusion were enriched, among all, in alanine, lipids, N-acetyl groups and phenylalanine and depleted in glutamine.ConclusionsSubject to validation in further larger studies, 1H-NMR metabonomics could be an effective and reliable ancillary tool for AE investigations and diagnosis particularly in acellular effusions.

Prediction of Potential Prognostic Tumor Markers of Re-ascites After Chemotherapy for Ovarian Cancer Based on LC/MS Method ​

“…Compared with urine and plasma, ascites is highly selective in ltering and concentrating plasma metabolites through the serosa. Therefore, ascites may become an ideal uid for the study of metabonomics for the discovery of biomarkers [18,19]. Recurrent ascites and rapid resistance to platinum therapy are common in advanced ovarian cancer.…”

A Metabolomic Analysis of Cirrhotic Ascites

“…This same technique was employed to investigate ascites associated with both reactive ovarian carcinoma and hepatocellular carcinoma (HCC). The ovarian carcinoma ascites was enriched in alanine, phenylalanine and lipids and depleted in glutamine [16]. A study of serum from cirrhotic ascites patients and healthy controls was reported, in which 12 amino acids and 8 conjugated bile acids were significantly increased in the ascites patients [17].…”

“…Attempts to distinguish cirrhotic, malignant, cardiac and inflammatory origins of ascites go back to the 1950s with paper chromatography used to measure proteins such as albumin, α1, α2 and È-globulin fractions [4], followed by radial immunodiffusion introduced in 1965. A total of Molecules 2022, 27, 3935 2 of 16 23 plasma proteins in ascites were determined using this methodology [5]. Lipoproteins [6] and bile acids [7] in ascites have also been determined in previous studies.…”

The Investigation of Metabonomic Pathways of Serum of Iranian Women with Recurrent Miscarriage Using 1H NMR

“…Liquid chromatography along with mass spectrometry (LC-MS), nuclear magnetic resonance (NMR), and gas chromatography MS (GC-MS) as some techniques is implemented to identify and quantify metabolites [ 13 ]. Metabolites such as plasma, urine, milk, saliva, amniotic fluid, tissue extracts, cerebrospinal fluid, bile, fecal extracts and semen can be detected by the above techniques [ 14 – 21 ].…”