24-HOUR TEMPORAL PATTERN OF NTPDase AND 5′-NUCLEOTIDASE ENZYMES IN RAT BLOOD SERUM

Detanico, Bernardo Carraro; Souza, Andressa de; Medeiros, Liciane Fernandes; Rozisky, Joanna Ripoll; Caumo, Wolnei; Hidalgo, María Paz Loayza; Battastini, Ana Maria Oliveira; Torres, Iraci Lucena da Silva

doi:10.3109/07420528.2010.512992

Cited by 9 publications

(20 citation statements)

References 52 publications

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“…It is interesting to note that a recent study from our group examining a possible temporal variation in the nucleotidases activities found an increase in ATP and ADP hydrolysis in rat blood serum during the dark (activity) phase [12]. During this phase, which is the time of greatest activity in rodents, there is an increase in sympathetic activity compared with the light (rest) phase, and consequently, increased circulating NE.…”

Section: Discussionmentioning

confidence: 95%

“…Previous work by our group demonstrated that ATPase and ADPase activities exhibit a circadian temporal pattern in rat blood serum, with the highest enzyme activities during the dark (activity) phase [12]. In contrast, 5′-nucleotidase did not show a temporal pattern.…”

Section: Introductionmentioning

confidence: 80%

“…In contrast, 5′-nucleotidase did not show a temporal pattern. The increase of ATP and ADP hydrolysis during the dark phase is accompanied by an increase in the secretion of melatonin and corticosterone [12], as well as an increase in sympathetic activity, since rodents (e.g., rats) are nocturnal animals and their activity increases during the dark phase.…”

Section: Introductionmentioning

confidence: 99%

“…ADP is a potent platelet aggregator [24], and it also promotes vasoconstriction [14]. Adenine nucleotides and nucleoside promote their physiological effects through binding to purinoceptors, which comprise P2 ionotropic ATP receptors (P 2 X 1-7 ) and the G-protein-coupled ATP/ ADP receptors (P 2 Y 1, 2, 4, 6,11,12,13,14 ), and P1 adenosine receptors (A 1 , 2A , 2B , and 3 ) [8].…”

Section: Introductionmentioning

confidence: 99%

“…Experimental design Rats were habituated to the maintenance room for 2 weeks before the beginning of the experiments. In order to investigate the role of physiological levels of melatonin, dexamethasone (mimicking endogenous corticosterone), and NE in ATP and ADP hydrolysis, the animals were killed by decapitation at approximately 0800 hours [light (rest) phase], since at this time the activities of the enzymes ATPase and ADPase are low compared to the dark (activity) phase [12], similar to the secretion of the hormones melatonin and corticosterone, and sympathetic activity. For this purpose, individual rats were transferred quickly to a separate room and decapitated within 1 min to obtain the blood samples.…”

Section: Introductionmentioning

confidence: 99%

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Physiological level of norepinephrine increases adenine nucleotides hydrolysis in rat blood serum

Detanico

Rozisky

Battastini

et al. 2011

Purinergic Signalling

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Extracellular adenosine 5'-triphosphate (ATP) and its breakdown products, adenosine 5'-diphosphate (ADP) and adenosine, have significant effects on a variety of biological processes. NTPDase enzymes, responsible for adenine nucleotides hydrolysis, are considered the major regulators of purinergic signaling in the blood. Previous work by our group demonstrated that ATP and ADP hydrolysis in rat blood serum are higher during the dark (activity) phase compared to the light (rest) phase. In nocturnal animals (e.g., rats), important physiological changes occur during the dark phase, such as increased circulating levels of melatonin, corticosterone, and norepinephrine (NE). This study investigated the physiological effects, in vivo and in vitro, of melatonin, dexamethasone, and NE upon nucleotides hydrolysis in rat blood serum. For in vivo experiments, the animals received a single injection of saline (control), melatonin (0.05 mg/kg), dexamethasone (0.1 mg/kg), or NE (0.03 mg/kg). For in vitro experiments, melatonin (1.0 nM), dexamethasone (1.0 μM), or NE (1.0 nM) was added directly to the reaction medium with blood serum before starting the enzyme assay. The results demonstrated that ATP and ADP hydrolysis in both in vitro and in vivo experiments were significantly higher with NE treatment compared to control (in vitro: ATP = 36.63%, ADP = 22.43%, P < 0.05; in vivo: ATP = 44.1%, ADP = 37.28%, P < 0.001). No significant differences in adenine nucleotides hydrolysis were observed with melatonin and dexamethasone treatments. This study suggests a modulatory role of NE in the nucleotidases pathway, decreasing extracellular ATP and ADP, and suggests that NE might modulate its own release by increasing the activities of soluble nucleotidases.

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Section: Discussionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 80%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Physiological level of norepinephrine increases adenine nucleotides hydrolysis in rat blood serum

Detanico

Rozisky

Battastini

et al. 2011

Purinergic Signalling

Self Cite

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Expression of ectonucleotidases in the prosencephalon of melatonin-proficient C3H and melatonin-deficient C57Bl mice: spatial distribution and time-dependent changes

et al. 2015

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Extracellular purines (ATP, ADP, AMP and adenosine) are important signaling molecules in the CNS. Levels of extracellular purines are regulated by enzymes located at the cell surface referred to as ectonucleotidases. Time-dependent changes in their expression could profoundly influence the availability of extracellular purines and thereby purinergic signaling. Using radioactive in situ hybridization, we analyzed the mRNA distribution of the enzymes NTPDase1, -2 and -3 and ecto-5'-nucleotidase in the prosencephalon of two mouse strains: melatonin-proficient C3H and melatonin-deficient C57Bl. The mRNAs of these enzymes were localized to specific brain regions, such as hippocampus, striatum, medial habenula and ventromedial hypothalamus. NTPDase3 expression was more widely distributed than previously thought. All ectonucleotidases investigated revealed a prominent time-dependent expression pattern. In C3H, the mRNA expression of all four enzymes gradually increased during the day and peaked during the night. In contrast, in C57Bl, ecto-5'-nucleotidase expression peaked at the beginning of the day and gradually decreased to trough levels at night. Recording of locomotor activity revealed higher daytime activity of C57Bl than of C3H. Our results indicate that the expression of ectonucleotidases varies according to time and genotype and suggest that melatonin exerts modulatory effects associated with different regulations of purinergic signaling in the brain. These findings provide an important basis for further examination of the complexity of the purinergic system in the brain.

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The circadian regulation of extracellular ATP

Wang

Dong²,

Hu³

et al. 2022

Purinergic Signalling

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Extracellular ATP is a potent signaling molecule released from various cells throughout the body and is intimately involved in the pathophysiological functions of the nervous system and immune system by activating P2 purinergic receptors. Recent increasingly studies showed that extracellular ATP exhibits circadian oscillation with an approximately 24-h periodicity, which participates in regulatory pathways of central oscillator suprachiasmatic nucleus and peripheral oscillator bladder, respectively. Oscillators modulate the protein expression of ATP release channels and ectonucleotidase activity through clock genes; indeed, real-time alterations of ATP release and degradation determine outcomes of temporal character on extracellular ATP rhythm. The regulatory pathways on extracellular ATP rhythm are different in central and peripheral systems. In this review, we summarize the circadian rhythm of extracellular ATP and discuss several circadian regulatory pathways in different organs via ATP release and degradation, to provide a new understanding for purinergic signaling in the regulatory mechanism of circadian rhythm and a potential target to research the circadian regulation of extracellular ATP in other circadian oscillators.

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24-HOUR TEMPORAL PATTERN OF NTPDase AND 5′-NUCLEOTIDASE ENZYMES IN RAT BLOOD SERUM

Cited by 9 publications

References 52 publications

Physiological level of norepinephrine increases adenine nucleotides hydrolysis in rat blood serum

Physiological level of norepinephrine increases adenine nucleotides hydrolysis in rat blood serum

Expression of ectonucleotidases in the prosencephalon of melatonin-proficient C3H and melatonin-deficient C57Bl mice: spatial distribution and time-dependent changes

The circadian regulation of extracellular ATP

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