(30) H2O2 modifies membrane structure and activity of acetylcholinesterase

Molochkina, E. M.; Zorina, O. M.; Fatkullina, L. D.; Goloschapov, Alexandre N.; Burlakova, E. B.

doi:10.1016/j.cbi.2005.10.075

Cited by 40 publications

(26 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In accordance to this, it has been reported that the addition of H 2 O 2 potentiated organophosphate toxicosis in chickens reducing the LD 50 of dichlorvos and diazinon, and reducing ChE activity, suggesting a toxic interaction between OPs and H 2 O 2 (Al-Baggou et al, 2011). This effect may be attributed to the prooxidant, which modulated ChE activity (Schallreuter et al, 2004;Molochkina et al, 2005). In the current study, combined exposure with tBOOH likewise reduced AChE expression and activity.…”

Section: Tablesupporting

confidence: 53%

“…For example an in vitro study showed that low concentrations of H 2 O 2 activate human recombinant AChE, whereas high concentrations inhibited the enzyme (Schallreuter et al, 2004). H 2 O 2 was also reported to modify erythrocyte membrane structure and activity of AChE (Molochkina et al, 2005). Activation/deactivation of skin AChE by H 2 O 2 was found to be a reversible oxidation process (Schallreuter and Elwary, 2007).…”

Section: Tablementioning

confidence: 99%

See 1 more Smart Citation

Impacts of oxidative stress on acetylcholinesterase transcription, and activity in embryos of zebrafish (Danio rerio) following Chlorpyrifos exposure

Rodrı́guez-Fuentes

Rubio-Escalante

Noreña-Barroso

et al. 2015

Comparative Biochemistry and Physiology Part C: Toxicology &

View full text Add to dashboard Cite

Section: Tablesupporting

confidence: 53%

Section: Tablementioning

confidence: 99%

Impacts of oxidative stress on acetylcholinesterase transcription, and activity in embryos of zebrafish (Danio rerio) following Chlorpyrifos exposure

Rodrı́guez-Fuentes

Rubio-Escalante

Noreña-Barroso

et al. 2015

Comparative Biochemistry and Physiology Part C: Toxicology &

View full text Add to dashboard Cite

“…Besides, several "in vitro" studies indicate that blood markers are negatively affected by increased oxidative stress, considering that the AChE activity is also inhibited by the increase of the reactive species (Molochkina et al, 2005). According to Milatovic, Gupta, and Aschner (2006) the agricultural pesticides promote the oxidative phosphorylation inhibition in the central nervous system, debilitating the cells capacity to keep their energy levels, what possibly increases the quantity of reactive oxygen species and reactive nitrogen species in the organism, characterizing the oxidative stress condition.…”

Section: Introductionmentioning

confidence: 99%

Effect of the Cymbopogon citratus Infusion on the Activity of Acetylcholinesterase Enzyme and on the Redox Profile in Farmers’ Erythrocytes

Mori¹,

Horn²,

Oliveira³

et al. 2017

JAS

View full text Add to dashboard Cite

Agrochemicals were more prominent in 1960, marked due to the agricultural modernization process. As a result of this widespread use for food production, there was also an increase in cases of intoxication caused by these agents which made it necessary to search for alternative therapies for agricultural workers. Thus, considering that phytochemical characterization revealed the presence of antioxidants in Cymbopogon citratus extract, the objective of this study was to evaluate the effect of this plant infusion on the enzyme acetylcholinesterase activity (AChE) and on the redox response in farmers' erythrocytes. These erytrocytes were processed and subjected to treatment with the Cymbopogon citratus infusion (5, 10, 25 and 50 g/L). In these samples the following were determined: the AChE enzyme activity, the levels of thiobarbituric acid reactive substances (TBARS), protein carbonyls (CPs) and reduced glutathione (GSH). In general, it was discovered that the inhibition of AChE activity is negative regarding to the increase of protein carbonyl levels and positive regarding the GSH levels. In addition, Cymbopogon citratus infusions could not even reverse this inhibition or the high levels of TBARS and CPs. On the other hand, levels of GSH were increased by infusions demonstrating the increased antioxidant activity in rural workers' erythrocytes.

show abstract

“…Many in vitro studies have indicated that several parameters of blood are negatively affected by increased oxidative stress and aging. It is also known that AChE activity is inhibited by free radicals and increased oxidative stress 10 . We have recently reported an age dependent increase in lipid peroxidation index MDA and a decline in the total antioxidant status of plasma 11,12 .…”

Section: Introductionmentioning

confidence: 99%

Age-Dependent Decline in Erythrocyte Acetylcholinesterase Activity: Correlation With Oxidative Stress

Jha¹,

Rizvi²

2009

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub

View full text Add to dashboard Cite

Background:Oxidative stress hypothesis offers a mechanism for the aging process and its involvement in other pathologies such as diabetes and neurodegenerative diseases like Alzheimer. AChE activity in erythrocytes may be considered as a marker of central cholinergic status. The present study was undertaken to (i) determine the activity of erythrocyte AChE as a function of human process (ii) correlate AChE activity with oxidative stress during human aging.Material and Methods: Blood was collected from healthy subjects (n = 37) 22-82 years. Erythrocyte AChE activity, MDA and plasma antioxidant capacity in terms of FRAP was measured spectrophotometrically.Results: There was a marked decrease in AChE activity with increasing age. The reduction in activity of AChE correlated well with increased lipid peroxidation and a decrease in FRAP values.Conclusion: Decreased antioxidant defense, and alteration in membrane rheology during aging process both may contribute towards decreased activity of AChE in erythrocyte membrane. This finding may help in explaining the neuronal complications taking place under conditions of oxidative stress, aging, and dementia.

show abstract

(30) H2O2 modifies membrane structure and activity of acetylcholinesterase

Cited by 40 publications

References 7 publications

Impacts of oxidative stress on acetylcholinesterase transcription, and activity in embryos of zebrafish (Danio rerio) following Chlorpyrifos exposure

Impacts of oxidative stress on acetylcholinesterase transcription, and activity in embryos of zebrafish (Danio rerio) following Chlorpyrifos exposure

Effect of the Cymbopogon citratus Infusion on the Activity of Acetylcholinesterase Enzyme and on the Redox Profile in Farmers’ Erythrocytes

Age-Dependent Decline in Erythrocyte Acetylcholinesterase Activity: Correlation With Oxidative Stress

Contact Info

Product

Resources

About