30 Role of Immunohistochemistry in Elucidating Lung Cancer Metastatic to the Ovary from Primary Ovarian Carcinoma

Robinson-Bennett, Bernice; Han, Aaron C.

doi:10.1016/s1874-5784(05)80116-3

Cited by 10 publications

(7 citation statements)

References 40 publications

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“…42 Around 40% of ovarian cancer is positive for VIM. 43 Differently to what was expected, we observed the down regulation of the VIM gene in 3D monoculture (0.38-fold smaller) when compared to 2D condition (Figure 4A). However, the association of SKOV-3 cells with mesenchymal cells (SKOV-3:MUC-9) promoted a 2.23-fold higher VIM expression, while the tendency to up regulation was observed only after the addition of fibroblast cells (SKOV-3:CCD-27Sk) (1.91-fold higher) (Figure 4B and 4C, respectively).…”

Section: Gene Expression Profile Of Skov-3 Cells Cultured In 2d and 3...contrasting

confidence: 79%

Generation of a Three-Dimensional in Vitro Ovarian Cancer Co-Culture Model for Drug Screening Assays

Tofani

Sousa

Luiz

et al. 2021

Journal of Pharmaceutical Sciences

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Section: Gene Expression Profile Of Skov-3 Cells Cultured In 2d and 3...contrasting

confidence: 79%

Generation of a Three-Dimensional in Vitro Ovarian Cancer Co-Culture Model for Drug Screening Assays

Tofani

Sousa

Luiz

et al. 2021

Journal of Pharmaceutical Sciences

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“…The positive results of vimentin indicated that the cells tested contain vimentin and can be interpreted as the cells that are bred in this study are lens epithelial cells. (13,14) There were correlation between the intensity of p63 expression in re-epithelialization, in response to injury. (15) In vitro wound healing models are used to uncover the process of cell migration.…”

Section: Discussionmentioning

confidence: 94%

Metformin Reduced Collagen Deposition and Contractility, but Increased Collagen Degradation in in vitro Posterior Capsule Opacification Model

et al. 2021

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BACKGROUND: Posterior capsule opacification (PCO) often occurs after cataract surgery. Metformin has been known to have an ability to inhibit fibrosis. This study aimed to investigate the effects of metformin on cell contractility, collagen deposition and degradation in human lens epithelial cells (HLEC) of cataract patients.METHODS: HLEC were isolated from the anterior lens capsule of patients undergoing cataract surgery. The HLEC culture was carried out using explant culture technique. The in vitro PCO model was created by scratching technique on HLEC cultures. The treatment groups were given 0.1, 0.5 and 1 mM metformin, respectively, while the control group were given 10% fetal bovine serum (FBS). On the 7th day after scratching technique, the collagen deposition, collagen degradation and cell contractility were evaluated.RESULTS: Collagen deposition in HLEC was significantly reduced after given 0.1 mM, 0.5 mM and 1 mM metformin (17.92±6.16 mg/mL, 12.92±4.31 mg/mL, 11.25±5.30 mg/mL, respectively), compared to the control group (31.46±7.52 μg/mL, p=0.002). Collagen degradation significantly was increased in the 0.1 mM, 0.5 mM and 1 mM metformin groups (4.77±9.27 mg/mL, 6.59±1.16 mg/mL, 6.35±1.90 mg/mL, respectively) compared to the control group (2.21±2.78 μg/mL, p=0.002). While, collagen contractility in HLEC was significantly reduced in 0.1mM, 0.5mM and 1 mM metformin groups (16.39±3.89%, 13.89±2.59%, 11.93±2.44%, respectively), compared to the control group (44.25±4.95%, p=0.000).CONCLUSION: Metformin reduced collagen deposition and contractility, but increased collagen degradation in HLEC of cataract patients through mechanism of extracellular matrix remodeling.KEYWORDS: metformin, human lens epithelial cell, fibrosis

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“…This result could be attributed to in‐vitro culture conditions. While Vimentin is normally the specific marker of mesenchymal cells, and is not usually present in normal epithelial cells, its expression was identified in epithelial cells under in‐vitro culture or in tumour cells of epithelial origin 64 . It has been found that primary keratinocytes were grown on the plate in serum‐free media with low concentrations of Ca +2 co‐express Vimentin and Keratins 65 .…”

Section: Discussionmentioning

confidence: 99%

“…While Vimentin is normally the specific marker of mesenchymal cells, and is not usually present in normal epithelial cells, its expression was identified in epithelial cells under in‐vitro culture or in tumour cells of epithelial origin. 64 It has been found that primary keratinocytes were grown on the plate in serum‐free media with low concentrations of Ca +2 co‐express Vimentin and Keratins. 65 A study on corneal epithelial cells identified that these cells express Keratins and Vimentin until they reach high confluency and form a 4–5 layered epithelium.…”

Section: Discussionmentioning

confidence: 99%

Enhancement of keratinocyte growth factor potential in inducing adipose‐derived stem cells differentiation into keratinocytes by collagen‐targeting

Amidzadeh

Yasami-Khiabani

Rahimi

et al. 2022

J Cellular Molecular Medi

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Different growth factors can regulate stem cell differentiation. We used keratinocyte growth factor (KGF) to direct adipose-derived stem cells (ASCs) differentiation into keratinocytes. To enhance KGF bioavailability, we targeted KGF for collagen by fusing it to collagen-binding domain from Vibrio mimicus metalloprotease (vibrioCBD-KGF). KGF and vibrioCBD-KGF were expressed in Escherichia coli and purified to homogeneity. Both proteins displayed comparable activities in stimulating proliferation of HEK-293 and MCF-7 cells. vibrioCBD-KGF demonstrated enhanced collagen-binding affinity in immunofluorescence and ELISA. KGF and vibrioCBD-KGF at different concentrations (2, 10, and 20 ng/ml) were applied for 21 days on ASCs cultured on collagen-coated plates. Keratinocyte differentiation was assessed based on morphological changes, the expression of keratinocyte markers (Keratin-10 and Involucrin), and stem cell markers (Collagen-I and Vimentin) by real-time PCR or immunofluorescence. Our results indicated that the expression of keratinocyte markers was substantially increased at all concentrations of vibrioCBD-KGF, while it was observed for KGF only at 20 ng/ml. Immunofluorescence staining approved this finding. Moreover, down-regulation of Collagen-I, an indicator of differentiation commitment, was more significant in samples treated with vibrioCBD-KGF. The present study showed that vibrioCBD-KGF is more potent in inducing the ASCs differentiation into keratinocytes compared to KGF. Our results have important implications for effective skin regeneration using collagen-based biomaterials.

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30 Role of Immunohistochemistry in Elucidating Lung Cancer Metastatic to the Ovary from Primary Ovarian Carcinoma

Cited by 10 publications

References 40 publications

Generation of a Three-Dimensional in Vitro Ovarian Cancer Co-Culture Model for Drug Screening Assays

Generation of a Three-Dimensional in Vitro Ovarian Cancer Co-Culture Model for Drug Screening Assays

Metformin Reduced Collagen Deposition and Contractility, but Increased Collagen Degradation in in vitro Posterior Capsule Opacification Model

Enhancement of keratinocyte growth factor potential in inducing adipose‐derived stem cells differentiation into keratinocytes by collagen‐targeting

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