31P MR spectroscopy and in vitro markers of oxidative capacity in type 2 diabetes patients

Praet, Stephan; Feyter, H.M.M.L. de; Jonkers, Richard A. M.; Nicolay, Klaas; Pul, Carola van; Kuipers, H.; Loon, Luc J. C. van; Prompers, Jeanine J.

doi:10.1007/s10334-006-0060-0

Cited by 30 publications

(27 citation statements)

References 52 publications

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“…The nature and the risks of the experimental procedures were explained to the subjects and all gave their written informed consent to participate in the study, which was approved by the local Medical Ethical Committee of the Máxima Medical Centre, Veldhoven, The Netherlands. This study is part of a larger project that studies mitochondrial function in chronic metabolic disease (33,34).…”

Section: Subjectsmentioning

confidence: 99%

“…31 P MRS was performed as described previously (33). In short, 31 P signals were collected using a 6 cm diameter surface coil placed over the M. vastus lateralis (spectral width, 2000 Hz; number of data points, 1024).…”

Section: P Mrsmentioning

confidence: 99%

“…Then, spectra were acquired during a rest-exercise-recovery protocol with a repetition time of 3 s and two scans yielding a time resolution of 6 s. The first 20 spectra (2 min) were measured at rest, after which the subjects started the exercise. Subjects performed a dynamic incremental single-leg extension exercise in the supine position using a homebuilt MR-compatible ergometer (33). The duration of the exercise varied per subject, but never exceeded 8 min, so that at least 5 min of recovery were recorded.…”

Section: P Mrsmentioning

confidence: 99%

“…The 31 P MRS data were analysed as described previously (33). In short, spectra were fitted in the time domain by using a non-linear least-squares algorithm (AMARES) in the jMRUI software package (40).…”

Section: P Mrs Data Analysismentioning

confidence: 99%

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Early or advanced stage type 2 diabetes is not accompanied by in vivo skeletal muscle mitochondrial dysfunction

Feyter¹,

Broek²,

Praet³

et al. 2008

European Journal of Endocrinology

107

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Objective: Several lines of evidence support a potential role of skeletal muscle mitochondrial dysfunction in the pathogenesis of insulin resistance and/or type 2 diabetes. However, it remains to be established whether mitochondrial dysfunction represents either cause or consequence of the disease. We examined in vivo skeletal muscle mitochondrial function in early and advanced stages of type 2 diabetes, with the aim to gain insight in the proposed role of mitochondrial dysfunction in the aetiology of insulin resistance and/or type 2 diabetes. Methods: Ten long-standing, insulin-treated type 2 diabetes patients, 11 subjects with impaired fasting glucose, impaired glucose tolerance and/or recently diagnosed type 2 diabetes, and 12 healthy, normoglycaemic controls, matched for age and body composition and with low habitual physical activity levels were studied. In vivo mitochondrial function of the vastus lateralis muscle was evaluated from post-exercise phosphocreatine (PCr) recovery kinetics using 31 P magnetic resonance spectroscopy (MRS). Intramyocellular lipid (IMCL) content was assessed in the same muscle using single-voxel 1 H MRS. Results: IMCL content tended to be higher in the type 2 diabetes patients when compared with normoglycaemic controls (PZ0.06). The 31 P MRS parameters for mitochondrial function, i.e. PCr and ADP recovery time constants and maximum aerobic capacity, did not differ between groups. Conclusions: The finding that in vivo skeletal muscle oxidative capacity does not differ between longstanding, insulin-treated type 2 diabetes patients, subjects with early stage type 2 diabetes and sedentary, normoglycaemic controls suggests that mitochondrial dysfunction does not necessarily represent either cause or consequence of insulin resistance and/or type 2 diabetes.European Journal of Endocrinology 158 643-653

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Section: Subjectsmentioning

confidence: 99%

Section: P Mrsmentioning

confidence: 99%

Section: P Mrsmentioning

confidence: 99%

Section: P Mrs Data Analysismentioning

confidence: 99%

See 2 more Smart Citations

Early or advanced stage type 2 diabetes is not accompanied by in vivo skeletal muscle mitochondrial dysfunction

Feyter¹,

Broek²,

Praet³

et al. 2008

European Journal of Endocrinology

107

View full text Add to dashboard Cite

show abstract

“…31 P-MRS was applied to measure postexercise phosphocreatine (PCr) recovery, as previously described (17). Results are expressed as the time constant of recovery for PCr and ADP, i.e., PCr and ADP , representing skeletal muscle oxidative capacity (18).…”

Section: Research Design Andmentioning

confidence: 99%

Exercise Training Improves Glycemic Control in Long-Standing Insulin-Treated Type 2 Diabetic Patients

et al. 2007

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Quantifying Skeletal Muscle Mitochondrial FunctionIn Vivoby³¹P Magnetic Resonance Spectroscopy

Kemp

2018

Mitochondrial Dysfunction Caused by Drugs and Environmental Toxicants

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31P MR spectroscopy and in vitro markers of oxidative capacity in type 2 diabetes patients

Cited by 30 publications

References 52 publications

Early or advanced stage type 2 diabetes is not accompanied by in vivo skeletal muscle mitochondrial dysfunction

Early or advanced stage type 2 diabetes is not accompanied by in vivo skeletal muscle mitochondrial dysfunction

Exercise Training Improves Glycemic Control in Long-Standing Insulin-Treated Type 2 Diabetic Patients

Quantifying Skeletal Muscle Mitochondrial FunctionIn Vivoby³¹P Magnetic Resonance Spectroscopy

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31P MR spectroscopy and in vitro markers of oxidative capacity in type 2 diabetes patients

Cited by 30 publications

References 52 publications

Early or advanced stage type 2 diabetes is not accompanied by in vivo skeletal muscle mitochondrial dysfunction

Early or advanced stage type 2 diabetes is not accompanied by in vivo skeletal muscle mitochondrial dysfunction

Exercise Training Improves Glycemic Control in Long-Standing Insulin-Treated Type 2 Diabetic Patients

Quantifying Skeletal Muscle Mitochondrial FunctionIn Vivoby31P Magnetic Resonance Spectroscopy

Contact Info

Product

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Quantifying Skeletal Muscle Mitochondrial FunctionIn Vivoby³¹P Magnetic Resonance Spectroscopy