344 Supplementing different forms of l-methionine and acetate alters the expression patterns of messenger RNA, proteins, and metabolites related to milk protein synthesis and energy metabolism in bovine mammary cells

Conejos, Jay Ronel V.; Jeon, Seungwoo; Bae, Moo-Ho; Lee, J. E.; Lee, B. S.; Park, J. S.; Moon, Jungyoon; Lee, H. G.

doi:10.2527/asasann.2017.344

Cited by 2 publications

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“…In that study, metabolite analysis showed that 12 metabolites were increase while UMP was decreased in the NALM-treated group. On the other hand, supplementing L-Met + acetatetreated group showed increases of 13 metabolites and decreases of IMP and pantothenate [111]. The Preprints (www.preprints.org) | NOT PEER-REVIEWED | Posted: 18 October 2018 doi:10.20944/preprints201810.0411.v1 effect of increasing supplemental Met according to the ideal 3:1 Lys to Met ratio related to protein synthesis and mTOR pathway-related proteins phosphorylation status has also been determined recently [112].…”

Section: Metabolomicsmentioning

confidence: 88%

“…One previous study has compared the efficacy of N-Acetyl-L-methionine (NALM), a conjugated form of L-methionine with acetate, and its digested forms on milk protein synthesis using an immortalized bovine mammary epithelial cell line [111]. NALM is a rumen-protected methionine that is cleaved into L-Met and acetate in small intestine or liver with high bioavailability (80% bypass rate).…”

Section: Metabolomicsmentioning

confidence: 99%

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Advances on Research in Protein-Enriched Cow’s Milk: A Review from Omics Perspective

Conejos¹,

H²

2018

Preprint

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With deep knowledge and numerous researches about DNA in addition to recent advances in molecular biology and omics analysis methodology, manipulation of DNA transcription becomes possible that may eventually affect protein expression. Possible posttranscriptional modifications such as phosphorylation of proteins could also greatly affect protein expression. There are vast evidences showing that synthesis of milk protein is strongly regulated by several factors such as insulin, amino acids and their specific amino acid transporters as well as various energy sources through transcriptional and posttranscriptional pathways with insulin-mTOR pathway serving as the central point. Available latest data suggest the possibility of nutrigenomic interventions to manipulate milk protein synthesis by supplying amino acids and different energy sources. This could greatly contribute to major progress in understanding milk protein synthesis. With improvement of 'omics' tools, utilization of these tools in our future studies would help us fully understand the mechanism behind. This could lead to possible control and manipulation of milk protein synthesis in mammary gland.

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Section: Metabolomicsmentioning

confidence: 88%

Section: Metabolomicsmentioning

confidence: 99%

Advances on Research in Protein-Enriched Cow’s Milk: A Review from Omics Perspective

Conejos¹,

H²

2018

Preprint

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“…A recent study compared the effect of acetate-protected L-methionine for milk protein synthesis in MAC-T cell line [16]. In this study, different treatments upregulate and downregulate several proteins and metabolic pathways involved in the process of energy and protein metabolism.…”

Section: Introductionmentioning

confidence: 99%

Supplementing L-Isoleucine Increases Medium Protein and Alters the Expression of Genes and Proteins Involved in Milk Protein Synthesis and Energy Metabolism in Bovine Mammary Cells

Conejos¹,

Lee²,

Park³

et al. 2019

Preprint

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The objective of this study was to determine the effects of supplementing L-isoleucine (L-Ile) on milk protein synthesis, using an immortalized bovine mammary epithelial (MAC-T) cell line. In this case, the cells were treated with 0, 0.3, 0.6, 0.9, 1.2 and 1.5 mM of supplemental Isoleucine (Ile), and the most efficient time for protein synthesis for each amino acid was determined by measuring the cell, medium and total protein at 0, 24, 48, 72 and 96 h. Confirmatory tests showed that 48h incubation time and 0.6 mM dosage of L-Ile are considered as the optimal time and dosage. The mechanism of milk protein synthesis was elucidated through proteomics analysis to clarify the metabolic pathway. When the L-Ile was supplemented, extracellular protein (medium protein) reached a peak at 48h, whereas in the case of the intracellular cell protein, it was shown to have reached to its peak at 24h in all L-Ile dosage treatments. In total, it is noted that there were 63 upregulated and 52 downregulated proteins. The results of the protein pathway analysis showed that the L-Ile group stimulated insulin/IGF pathway-mitogen activated protein kinase kinase/MAP kinase cascade, insulin/IGF pathway-protein kinase B signaling cascade, p53 pathway, de novo purine biosynthesis, Wnt signaling pathway, glycolysis, pentose phosphate pathway, and ATP synthesis which are pathways involved and related to protein and energy metabolism. Together, these results demonstrate that L-Ile supplementation was effective in stimulating β-casein synthesis by stimulating genes and pathways which are significantly related to protein and energy metabolism.

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344 Supplementing different forms of l-methionine and acetate alters the expression patterns of messenger RNA, proteins, and metabolites related to milk protein synthesis and energy metabolism in bovine mammary cells

Cited by 2 publications

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Advances on Research in Protein-Enriched Cow’s Milk: A Review from Omics Perspective

Advances on Research in Protein-Enriched Cow’s Milk: A Review from Omics Perspective

Supplementing L-Isoleucine Increases Medium Protein and Alters the Expression of Genes and Proteins Involved in Milk Protein Synthesis and Energy Metabolism in Bovine Mammary Cells

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344 Supplementing different forms of l-methionine and acetate alters the expression patterns of messenger RNA, proteins, and metabolites related to milk protein synthesis and energy metabolism in bovine mammary cells

Cited by 2 publications

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Advances on Research in Protein-Enriched Cow&rsquo;s Milk: A Review from Omics Perspective

Advances on Research in Protein-Enriched Cow&rsquo;s Milk: A Review from Omics Perspective

Supplementing L-Isoleucine Increases Medium Protein and Alters the Expression of Genes and Proteins Involved in Milk Protein Synthesis and Energy Metabolism in Bovine Mammary Cells

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Advances on Research in Protein-Enriched Cow’s Milk: A Review from Omics Perspective

Advances on Research in Protein-Enriched Cow’s Milk: A Review from Omics Perspective