3D Bioprinted GelMA/PEGDA Hybrid Scaffold for Establishing an In Vitro Model of Melanoma

Duan, Jiahui; Cao, Yanyan; Shen, Zhizhong; Cheng, Yongqiang; Ma, Zhuwei; Wang, Lijing; Zhang, Yating; An, Yuchuan; Sang, Shengbo

doi:10.4014/jmb.2111.11003

Cited by 25 publications

(14 citation statements)

References 34 publications

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“…Similar spheroid morphology was also found for A375 cells cultured in alginate-based hydrogel, 48 liquid-overlay method, 49 and other GelMA-based scaffolds. 50 The spheroid formation bigger than 500 μm could cause limited diffusion of oxygen and nutrients to cells, resulting in populations of proliferative, quiescent, and necrotic cells. 51,52 However, the cell necrosis was not observed in this study based on the continues cellular proliferation and upregulation of Ki67 gene expression from Day 5 to Day 7, which may since the cellular spheroid size did not reach 500 μm at Day 5, and longer culture time, like 10−15 days may be needed to observe the cellular necrosis.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Effect of Fibroblasts on Melanoma Metastasis Using a Biomimetic Co-Culture Model

Yang

et al. 2023

ACS Biomater. Sci. Eng.

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Melanoma is a highly malignant tumor originating from melanocytes. The 5-year survival rate of primary melanoma is 98%, whereas the survival rate of metastatic melanoma is only 10%, which can be attributed to the insensitivity to existing treatments. Fibroblasts are the primary cells in the dermis that promote melanoma metastasis; however, the molecular mechanism underlying the fibroblast–melanoma interaction is yet to be completely understood. Herein, gelatin methacryloyl (GelMA) was used to construct a co-culture model for melanoma cells (A375) and fibroblasts. GelMA retains the good biological properties of collagen, which has been identified as the primary component of the melanoma tumor microenvironment. Fibroblasts were encapsulated in GelMA, whereas A375 cells were cultured on the GelMA surface, which realistically mimics the macrostructure of melanoma. A375 cells co-cultured with fibroblasts demonstrated a higher cellular proliferation rate, potentials of neoneurogenesis, overexpression of epithelial mesenchymal transition markers, and a faster migration rate compared with A375 cells cultured alone, which could be due to the cancer-associated fibroblast activation and the overexpression of transforming growth factor β1 and fibroblast growth factor-2 by fibroblasts. Overall, this study revealed the possible mechanisms of fibroblast–melanoma interaction and suggested that this co-culture model could be potentially further developed as a platform for screening chemotherapies in the future.

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Section: Discussionmentioning

confidence: 99%

Evaluation of the Effect of Fibroblasts on Melanoma Metastasis Using a Biomimetic Co-Culture Model

Yang

et al. 2023

ACS Biomater. Sci. Eng.

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“…The data obtained were validated using a mouse xenograft model and it was found that the 3D in vitro method helps in the understanding of tumorigenesis and the associated tumor micro-environment [136]. A 3D bioprinted GelMA/PEGDA hybrid scaffold mimicked the tumor micro-environment of human malignant melanoma cell and was reported to be suitable for the expansion and differentiation of tumor cells; additionally, tumor cells were growing faster and exhibited drug-resistant potential [137]. Alginate and gelatin bioprintable hydrogel together with BC cells and fibroblast were printed to form a 3D mimicking tumor micro-environment.…”

Section: Tumor Microenvironment and 3d Printingmentioning

confidence: 94%

Three-Dimensional (3D) Printing in Cancer Therapy and Diagnostics: Current Status and Future Perspectives

Safhi

2022

Pharmaceuticals

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Three-dimensional (3D) printing is a technique where the products are printed layer-by-layer via a series of cross-sectional slices with the exact deposition of different cell types and biomaterials based on computer-aided design software. Three-dimensional printing can be divided into several approaches, such as extrusion-based printing, laser-induced forward transfer-based printing systems, and so on. Bio-ink is a crucial tool necessary for the fabrication of the 3D construct of living tissue in order to mimic the native tissue/cells using 3D printing technology. The formation of 3D software helps in the development of novel drug delivery systems with drug screening potential, as well as 3D constructs of tumor models. Additionally, several complex structures of inner tissues like stroma and channels of different sizes are printed through 3D printing techniques. Three-dimensional printing technology could also be used to develop therapy training simulators for educational purposes so that learners can practice complex surgical procedures. The fabrication of implantable medical devices using 3D printing technology with less risk of infections is receiving increased attention recently. A Cancer-on-a-chip is a microfluidic device that recreates tumor physiology and allows for a continuous supply of nutrients or therapeutic compounds. In this review, based on the recent literature, we have discussed various printing methods for 3D printing and types of bio-inks, and provided information on how 3D printing plays a crucial role in cancer management.

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“…used 3D bioprinting to construct GelMA-polyethylene (glycol) diacrylate composite scaffolds for human malignant melanoma cell growth. The results showed a high aggregation, expansion and differentiation of melanoma cells on the bioprinted scaffolds [ 160 ]. Similarly, Jeong et al .…”

Section: Applications Of 3d Skin Bioprintingmentioning

confidence: 99%

Advances in 3D skin bioprinting for wound healing and disease modeling

Zhang

Zhao

et al. 2022

Regenerative Biomaterials

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Even with many advances in design strategies over the past three decades, an enormous gap remains between existing tissue engineering skin and natural skin. Currently available in vitro skin models still cannot replicate the three-dimensionality and heterogeneity of the dermal microenvironment sufficiently to recapitulate many of the known characteristics of skin disorder or disease in vivo. Three-dimensional (3D) bioprinting enables precise control over multiple compositions, spatial distributions, and architectural complexity, therefore offering hope for filling the gap of structure and function between natural and artificial skin. Our understanding of wound healing process and skin disease would thus be boosted by the development of in vitro models that could more completely capture the heterogeneous features of skin biology. Here we provide an overview of recent advances in 3D skin bioprinting, as well as design concepts of cells and bioinks suitable for the bioprinting process. We focus on the applications of this technology for engineering physiological or pathological skin model, focusing more specifically on the function of skin appendages and vasculature. We conclude with current challenges and the technical perspective for further development of 3D skin bioprinting.

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3D Bioprinted GelMA/PEGDA Hybrid Scaffold for Establishing an In Vitro Model of Melanoma

Cited by 25 publications

References 34 publications

Evaluation of the Effect of Fibroblasts on Melanoma Metastasis Using a Biomimetic Co-Culture Model

Evaluation of the Effect of Fibroblasts on Melanoma Metastasis Using a Biomimetic Co-Culture Model

Three-Dimensional (3D) Printing in Cancer Therapy and Diagnostics: Current Status and Future Perspectives

Advances in 3D skin bioprinting for wound healing and disease modeling

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