3D bioprinting of human chondrocyte-laden nanocellulose hydrogels for patient-specific auricular cartilage regeneration

Ávila, Héctor Martínez; Schwarz, Silke; Rotter, Nicole; Gatenholm, Paul

doi:10.1016/j.bprint.2016.08.003

Cited by 236 publications

(146 citation statements)

References 60 publications

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“…We concluded that NFC/A bioinks were suitable for bioprinting iPSCs to support cartilage production in co-culture with irradiated chondrocytes. We noticed that after printing, viable cells increased in number over time, which was in agreement with previous observations with other cell types that human chondrocytes bioprinted in noncytotoxic, nanocellulose-based bioink exhibited cell viabilities of 73% and 86% after 1 and 7 days of 3D culture, respectively 26, 27 . The positive co-culture effect supported earlier findings that irradiated chondrocytes would stimulate iPSC differentiation by cell-to-cell contact combined with local inherent growth factors 8, 28 .…”

Section: Discussionsupporting

confidence: 91%

Cartilage Tissue Engineering by the 3D Bioprinting of iPS Cells in a Nanocellulose/Alginate Bioink

Nguyen

Hägg

Forsman

et al. 2017

Sci Rep

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378

308

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Cartilage lesions can progress into secondary osteoarthritis and cause severe clinical problems in numerous patients. As a prospective treatment of such lesions, human-derived induced pluripotent stem cells (iPSCs) were shown to be 3D bioprinted into cartilage mimics using a nanofibrillated cellulose (NFC) composite bioink when co-printed with irradiated human chondrocytes. Two bioinks were investigated: NFC with alginate (NFC/A) or hyaluronic acid (NFC/HA). Low proliferation and phenotypic changes away from pluripotency were seen in the case of NFC/HA. However, in the case of the 3D-bioprinted NFC/A (60/40, dry weight % ratio) constructs, pluripotency was initially maintained, and after five weeks, hyaline-like cartilaginous tissue with collagen type II expression and lacking tumorigenic Oct4 expression was observed in 3D -bioprinted NFC/A (60/40, dry weight % relation) constructs. Moreover, a marked increase in cell number within the cartilaginous tissue was detected by 2-photon fluorescence microscopy, indicating the importance of high cell densities in the pursuit of achieving good survival after printing. We conclude that NFC/A bioink is suitable for bioprinting iPSCs to support cartilage production in co-cultures with irradiated chondrocytes.

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Section: Discussionsupporting

confidence: 91%

Cartilage Tissue Engineering by the 3D Bioprinting of iPS Cells in a Nanocellulose/Alginate Bioink

Nguyen

Hägg

Forsman

et al. 2017

Sci Rep

Self Cite

378

308

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“…Cellulose can be found as nano-crystals, nanofibers, or in bacterial form, where they can have high surface area. Nano-cellulose crystals and fibers have been widely mixed with alginate to form bioinks which have excellent shear-thinning properties allowing improvement in printability as well as fast cross-linking to achieve shape fidelity [244,[266][267][268][269][270][271][272]. For light-assisted printing, cellulose has been mainly used as a filler and reinforcement material to stiffen and strengthen hydrogels and soft polymers.…”

Section: Process and Materialsmentioning

confidence: 99%

“…The encapsulation of chondrocytes for cartilage tissue engineering [244,269,271], human derived induced pluripotent stem cells [266], human bone marrow derived mesenchymal stem cells [272], pancreatic cancer cells [268], and fibroblast and hepatoma cells [270], all showed increased bioactivity such as cell expression, proliferation, and viability. However, a more detailed study of printing process parameters show that optimum pressure, shear stress, and nozzle size can greatly affect the bioactivity of the encapsulated cells, i.e., for nozzle diameters below 400 µm, cell morphology and proliferation suffered, while for nozzle diameters below 200 µm cell viability also was affected [267].…”

Section: Biocompatibility Biodegradability and Bioactivitymentioning

confidence: 99%

Engineered 3D Polymer and Hydrogel Microenvironments for Cell Culture Applications

2019

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The realization of biomimetic microenvironments for cell biology applications such as organ-on-chip, in vitro drug screening, and tissue engineering is one of the most fascinating research areas in the field of bioengineering. The continuous evolution of additive manufacturing techniques provides the tools to engineer these architectures at different scales. Moreover, it is now possible to tailor their biomechanical and topological properties while taking inspiration from the characteristics of the extracellular matrix, the three-dimensional scaffold in which cells proliferate, migrate, and differentiate. In such context, there is therefore a continuous quest for synthetic and nature-derived composite materials that must hold biocompatible, biodegradable, bioactive features and also be compatible with the envisioned fabrication strategy. The structure of the current review is intended to provide to both micro-engineers and cell biologists a comparative overview of the characteristics, advantages, and drawbacks of the major 3D printing techniques, the most promising biomaterials candidates, and the trade-offs that must be considered in order to replicate the properties of natural microenvironments.

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“…For instance, CELLINK®, a nanocellulose hydrogel-based bio-ink, has been tested by Gatenholm et al for application in auricular cartilage tissue to provide an alternative and effective treatment for serious diseases. 158 '3D' printing technology makes CNF very promising as a product that could revolutionize the manufacturing industry. 159 Electrospun cellulose nanofibers (ECNF) can be produced from cellulose, cellulosic pulps or its derivatives.…”

Section: Nanocellulosementioning

confidence: 99%

High value‐added monomer chemicals and functional bio‐based materials derived from polymeric components of lignocellulose by organosolv fractionation

Zhang

Cai

Qin

et al. 2019

Biofuels Bioprod Bioref

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Organosolv fractionation (OF) is a promising method for lignocellulosic biomass biorefining to produce biofuels, biochemicals, and biomaterials. The fractionated polymeric components may be good feedstocks to produce bio‐based materials such as green polymers via chemical or biological conversion. However, related work to specify the bio‐based materials production via organosolv fractionation of lignocellulosic biomass is relatively scarce. In this work we have provided a comprehensive review of typical organosolv fractionation for isolating cellulose, hemicelluloses, and lignin, as well as the high value‐added monomer chemicals and functional materials derived from the fractionated components and their potential applications developed in recent years, primarily including the modified cellulose fibers for polymer materials, cellulose nanocrystals (CNCs), polysaccharide‐derived platform precursors for synthesis of bio‐based polymers, lignin‐derived dispersants, surfactants, carbon materials, and hemicellulose‐derived functional materials, etc. This work suggests that organosolv fractionation of lignocellulosic biomass could be a good entry to biomass refinery for high value‐added and functional materials production. However, there are still many challenges that should be overcome in terms of the fundamental, technical, and economic aspects for the organosolv fractionation process, formation of precursor compounds, synthesis of the bio‐based materials, design of product properties and functions, and the integration of the entire process. © 2019 Society of Chemical Industry and John Wiley & Sons, Ltd

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3D bioprinting of human chondrocyte-laden nanocellulose hydrogels for patient-specific auricular cartilage regeneration

Cited by 236 publications

References 60 publications

Cartilage Tissue Engineering by the 3D Bioprinting of iPS Cells in a Nanocellulose/Alginate Bioink

Cartilage Tissue Engineering by the 3D Bioprinting of iPS Cells in a Nanocellulose/Alginate Bioink

Engineered 3D Polymer and Hydrogel Microenvironments for Cell Culture Applications

High value‐added monomer chemicals and functional bio‐based materials derived from polymeric components of lignocellulose by organosolv fractionation

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