3D bioprinting of structural proteins

Włodarczyk‐Biegun, Małgorzata K.; Campo, Aránzazu del

doi:10.1016/j.biomaterials.2017.04.019

Cited by 156 publications

(118 citation statements)

References 125 publications

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“…Polysaccharides with fast gelation properties are appropriate for the fabrication of complex 3D scaffolds with various morphologies, such as microfibers, microspheres, membranes, and defined blocks, thus providing architectural cues for tissue/organ constructs in vitro. The natural proteins used as cell culture matrices are collagen, gelatin, fibrin, silk protein (mainly referring to silk fibroin), poly‐ l ‐lysine (PLL), and soy protein . These proteins can interact with cells and supply physiologically relevant microenvironments to guide cellular behaviors by presenting componential cues .…”

Section: Snapshot Of Hydrogelsmentioning

confidence: 99%

Advances in Hydrogels in Organoids and Organs‐on‐a‐Chip

et al. 2019

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The major motivation is to better mimic human physiology and functions at multiscales from the molecular to the cellular, tissue, organ or even whole organism level. Current model systems primarily rely on the monolayer cell cultures and animal models. Simplistic monolayer cultures have their advantages, but they are often significantly different in gene expression, epigenetics, and cell function compared to native 3D tissues. [1,2] Also, they often lack cell-cell and cell-matrix interactions, [2,3] leading to the absence of tissue specific properties. Although animal models are widely used in biomedical research, they fail to faithfully predict human responses in a physiologically relevant manner due to the significant species divergences. [4] The limitations of these systems have provided an impetus for the development of alternative cell-based 3D models in vitro that better resemble the complex functionalities of living organs. Over the last several years, organoids and organ-on-a-chip (OOC), representing the major technological breakthrough, have emerged as two distinct model systems to achieve the same goal of building 3D organotypic models in vitro by bridging the gap between animal models and monolayer cultures. These engineered models are different in terms of cell source, tissue composition, architectural variability, functional features, scales, cellular fidelity, etc. Organoids, primarily evolving from developmental principles, refer to 3D multicellular tissues by self-organization of stem cells or organ-specific progenitors, which can recapitulate the intricate architectures and functionalities of in vivo organs. [5][6][7] Recent breakthroughs in organoid technology have enabled the successful generation of a variety of human organoids, such as the brain, [8,9] intestine, [10,11] liver, [12] kidney, [13,14] lung, [15] etc. These near physiological 3D organoids have garnered momentum for their potential applications in human organ development and disease modeling, drug screening and regenerative medicine. The explosion of organoids research has been catalyzed by the significant progress of stem cell biology and availability of human stem cells. In contrast, the OOC, relying on the bioengineering design principle, is a miniaturized in vitro model that can recreate the functional units of living organs on a microfluidic cell culture device using predifferentiated cells, often cell lines. [1,16] The OOC is primarily evolved from the convergence of tissue engineering and microfabricated technologies, which is characterized by the capability to simulate cellular microenvironment by precise control over fluid flow, mechanical forces and biochemical factors. [4,17] Remarkable progress has been made Significant advances in materials, microscale technology, and stem cell biology have enabled the construction of 3D tissues and organs, which will ultimately lead to more effective diagnostics and therapy. Organoids and organs-on-a-chip (OOC), evolved from developmental biology and bioengineering principles, have e...

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Section: Snapshot Of Hydrogelsmentioning

confidence: 99%

Advances in Hydrogels in Organoids and Organs‐on‐a‐Chip

et al. 2019

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“…Bioinks loaded with polysaccharides and proteins (e.g., collagen, alginate) can show very different values of viscosity. The technology is mature with available bioprinters on the market at reasonable costs, which allow customization and focus on bioink mixtures [45][46][47][48][49][50][51][52][53].…”

Section: Process and Materialsmentioning

confidence: 99%

“…Proteins and peptides are highly bioactive molecules which perform specific biological functions, many of them are part of the natural extracellular matrix, and they are widely employed in bioprinting applications [45,287].…”

Section: Proteinsmentioning

confidence: 99%

Engineered 3D Polymer and Hydrogel Microenvironments for Cell Culture Applications

2019

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The realization of biomimetic microenvironments for cell biology applications such as organ-on-chip, in vitro drug screening, and tissue engineering is one of the most fascinating research areas in the field of bioengineering. The continuous evolution of additive manufacturing techniques provides the tools to engineer these architectures at different scales. Moreover, it is now possible to tailor their biomechanical and topological properties while taking inspiration from the characteristics of the extracellular matrix, the three-dimensional scaffold in which cells proliferate, migrate, and differentiate. In such context, there is therefore a continuous quest for synthetic and nature-derived composite materials that must hold biocompatible, biodegradable, bioactive features and also be compatible with the envisioned fabrication strategy. The structure of the current review is intended to provide to both micro-engineers and cell biologists a comparative overview of the characteristics, advantages, and drawbacks of the major 3D printing techniques, the most promising biomaterials candidates, and the trade-offs that must be considered in order to replicate the properties of natural microenvironments.

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“…In particular, a recent study created silk/poly(ethylene glycol) (PEG) bioink gel that could be 3D printed and created self-standing gel scaffolds (Zheng et al, 2018). However, 3D printing of silk proteins has been known to cause nozzle clogging, making it challenging to 3D print silk substrates (Włodarczyk-Biegun & Del Campo, 2017). Similarly in another study, fibroin and Poly(ethylene oxide) (PEO) were blended and spun into fibers using electrospinning to increase ease of production while preserving the biocompatibility (Jin, Fridrikh, Rutledge, & Kaplan, 2002).…”

Section: Processing Of Silk Fibroin Into Different Materials Formatsmentioning

confidence: 99%

Microscopic considerations for optimizing silk biomaterials

DeBari

Abbott

2018

WIREs Nanomed Nanobiotechnol

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Silk is an especially appealing biomaterial due to its adaptable mechanical properties, allowing it to be used in a wide range of tissue engineering applications. However, processing conditions play a critical role in determining silk's mechanical properties, biodegradability, and biocompatibility. While bulk properties of silk have been widely explored, focusing on microscopic features is becoming increasingly important, as modifications at this scale largely affect the resulting regenerative properties of the biomaterial. Structural changes caused by the silk source, extraction, and processing should be carefully considered, as they will affect the biocompatibility and degradability of silk fibroin. Processing techniques and physical properties of silk that make it an ideal material for many biomedical applications will be explored. This article is categorized under: Implantable Materials and Surgical Technologies > Nanotechnology in Tissue Repair and Replacement Implantable Materials and Surgical Technologies > Nanomaterials and Implants.

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3D bioprinting of structural proteins

Cited by 156 publications

References 125 publications

Advances in Hydrogels in Organoids and Organs‐on‐a‐Chip

Advances in Hydrogels in Organoids and Organs‐on‐a‐Chip

Engineered 3D Polymer and Hydrogel Microenvironments for Cell Culture Applications

Microscopic considerations for optimizing silk biomaterials

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