3D poly-ε-caprolactone/graphene porous scaffolds for bone tissue engineering

Huang, Huei Yu; Fan, Fang Yu; Shen, Yung Kang; Wang, Chia Hsien; Huang, Yuen Tzu; Chern, Ming Jyh; Wang, Yen Hsiang; Wang, Liping

doi:10.1016/j.colsurfa.2020.125393

Cited by 24 publications

(21 citation statements)

References 32 publications

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“…However, no significant differences were observed between PCL/GPN groups. These results are in accordance with the literature, in which polymeric scaffolds increase hydrophilicity by GPN incorporation [52,57,58].…”

Section: Discussionsupporting

confidence: 93%

Additive Manufactured Poly(ε-caprolactone)-graphene Scaffolds: Lamellar Crystal Orientation, Mechanical Properties and Biological Performance

et al. 2022

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Understanding the mechano–biological coupling mechanisms of biomaterials for tissue engineering is of major importance to assure proper scaffold performance in situ. Therefore, it is of paramount importance to establish correlations between biomaterials, their processing conditions, and their mechanical behaviour, as well as their biological performance. With this work, it was possible to infer a correlation between the addition of graphene nanoparticles (GPN) in a concentration of 0.25, 0.5, and 0.75% (w/w) (GPN0.25, GPN0.5, and GPN0.75, respectively) in three-dimensional poly(ε-caprolactone) (PCL)-based scaffolds, the extrusion-based processing parameters, and the lamellar crystal orientation through small-angle X-ray scattering experiments of extruded samples of PCL and PCL/GPN. Results revealed a significant impact on the scaffold’s mechanical properties to a maximum of 0.5% of GPN content, with a significant improvement in the compressive modulus of 59 MPa to 93 MPa. In vitro cell culture experiments showed the scaffold’s ability to support the adhesion and proliferation of L929 fibroblasts (fold increase of 28, 22, 23, and 13 at day 13 (in relation to day 1) for PCL, GPN0.25, GPN0.5, and GPN0.75, respectively) and bone marrow mesenchymal stem/stromal cells (seven-fold increase for all sample groups at day 21 in relation to day 1). Moreover, the cells maintained high viability, regular morphology, and migration capacity in all the different experimental groups, assuring the potential of PCL/GPN scaffolds for tissue engineering (TE) applications.

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Section: Discussionsupporting

confidence: 93%

Additive Manufactured Poly(ε-caprolactone)-graphene Scaffolds: Lamellar Crystal Orientation, Mechanical Properties and Biological Performance

et al. 2022

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“…and it was confirmed that the mechanical properties, cell attachment, and proliferation were improved with a higher ratio of GR [141]. Figure 6.…”

Section: Graphene-based Materialssupporting

confidence: 59%

“…GR platelets as fillers, NaCl as a porogenic material and PCL as a matrix were used to produce porous scaffolds using the solvent-casting/particulate-leaching method. The preparation process and products are shown in Figure 6 [141]. The compressive strength, porosity, contact angle, weight loss, and variations in pH values in degradation tests as well as the biocompatibility by seeding osteoblast-like (MG-63) cells in vitro were studied, and it was confirmed that the mechanical properties, cell attachment, and proliferation were improved with a higher ratio of GR [141].…”

Section: Graphene-based Materialsmentioning

confidence: 88%

Advances in Use of Nanomaterials for Musculoskeletal Regeneration

Jampílek

Plachá

2021

Pharmaceutics

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Since the worldwide incidence of bone disorders and cartilage damage has been increasing and traditional therapy has reached its limits, nanomaterials can provide a new strategy in the regeneration of bones and cartilage. The nanoscale modifies the properties of materials, and many of the recently prepared nanocomposites can be used in tissue engineering as scaffolds for the development of biomimetic materials involved in the repair and healing of damaged tissues and organs. In addition, some nanomaterials represent a noteworthy alternative for treatment and alleviating inflammation or infections caused by microbial pathogens. On the other hand, some nanomaterials induce inflammation processes, especially by the generation of reactive oxygen species. Therefore, it is necessary to know and understand their effects in living systems and use surface modifications to prevent these negative effects. This contribution is focused on nanostructured scaffolds, providing a closer structural support approximation to native tissue architecture for cells and regulating cell proliferation, differentiation, and migration, which results in cartilage and bone healing and regeneration.

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“…The advantage of this technique is that the produced scaffold will exhibit a high porosity (up to 90%) with the capability to tune the pore size. 186,187 A gas foaming method was developed to address the solvent toxicity issue, which is also the main drawback of the solvent casting technique. This method uses carbon dioxide gas as a porogen to make the porous structure of a scaffold.…”

Section: D Nanomaterials-based Scaffolds Formentioning

confidence: 99%

“…Solvent casting is a simple and cost-effective scaffold fabrication technique by adding a combination of solvent (2D nanomaterials solution) and uniformly distributed salt particles (e.g., NaCl) into a polymer solution. The advantage of this technique is that the produced scaffold will exhibit a high porosity (up to 90%) with the capability to tune the pore size. , A gas foaming method was developed to address the solvent toxicity issue, which is also the main drawback of the solvent casting technique. This method uses carbon dioxide gas as a porogen to make the porous structure of a scaffold.…”

Section: D Nanomaterials-based Scaffolds For Neural Tissue Engineeringmentioning

confidence: 99%

Recent Advances in the Application of Two-Dimensional Nanomaterials for Neural Tissue Engineering and Regeneration

Halim

Zhang

et al. 2021

ACS Biomater. Sci. Eng.

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The complexity of the nervous system structure and function, and its slow regeneration rate, makes it more difficult to treat compared to other tissues in the human body when an injury occurs. Moreover, the current therapeutic approaches including the use of autografts, allografts, and pharmacological agents have several drawbacks and can not fully restore nervous system injuries. Recently, nanotechnology and tissue engineering approaches have attracted many researchers to guide tissue regeneration in an effective manner. Owing to their remarkable physicochemical and biological properties, two-dimensional (2D) nanomaterials have been extensively studied in the tissue engineering and regenerative medicine field. The great conductivity of these materials makes them a promising candidate for the development of novel scaffolds for neural tissue engineering application. Moreover, the high loading capacity of 2D nanomaterials also has attracted many researchers to utilize them as a drug/gene delivery method to treat various devastating nervous system disorders. This review will first introduce the fundamental physicochemical properties of 2D nanomaterials used in biomedicine and the supporting biological properties of 2D nanomaterials for inducing neuroregeneration, including their biocompatibility on neural cells, the ability to promote the neural differentiation of stem cells, and their immunomodulatory properties which are beneficial for alleviating chronic inflammation at the site of the nervous system injury. It also discusses various types of 2D nanomaterials-based scaffolds for neural tissue engineering applications. Then, the latest progress on the use of 2D nanomaterials for nervous system disorder treatment is summarized. Finally, a discussion of the challenges and prospects of 2D nanomaterials-based applications in neural tissue engineering is provided.

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3D poly-ε-caprolactone/graphene porous scaffolds for bone tissue engineering

Cited by 24 publications

References 32 publications

Additive Manufactured Poly(ε-caprolactone)-graphene Scaffolds: Lamellar Crystal Orientation, Mechanical Properties and Biological Performance

Additive Manufactured Poly(ε-caprolactone)-graphene Scaffolds: Lamellar Crystal Orientation, Mechanical Properties and Biological Performance

Advances in Use of Nanomaterials for Musculoskeletal Regeneration

Recent Advances in the Application of Two-Dimensional Nanomaterials for Neural Tissue Engineering and Regeneration

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