3D printed lactose-crosslinked gelatin scaffolds as a drug delivery system for dexamethasone

Long, Jingjunjiao; Etxabide, Alaitz; Caba, Koro de la; Seyfoddin, Ali

doi:10.1016/j.eurpolymj.2019.02.019

Cited by 38 publications

(14 citation statements)

References 30 publications

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“…This can be attributed to the highly macroporous behaviour of the scaffolds as well as the hydrophilic nature of the dexamethasone disodium phosphate incorporated in the scaffolds. This was in line with previously reported release of highly water soluble dexamethasone disodium phosphate from crosslinked gelatin scaffolds 67 . However, the release profiles for the hydrophobic bioactive, curcumin, were very different from that of dexamethasone and closely followed the results obtained from matrix hydration data discussed above.…”

Section: Porositometric Evaluation Of the Cht/mpegcryosponges To Devsupporting

confidence: 93%

Macroporous chitosan/methoxypoly(ethylene glycol) based cryosponges with unique morphology for tissue engineering applications

Kumar

Pillay

Choonara

2021

Sci Rep

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Three-dimensional porous scaffolds are widely employed in tissue engineering and regenerative medicine for their ability to carry bioactives and cells; and for their platform properties to allow for bridging-the-gap within an injured tissue. This study describes the effect of various methoxypolyethylene glycol (mPEG) derivatives (mPEG (-OCH3 functionality), mPEG-aldehyde (mPEG-CHO) and mPEG-acetic acid (mPEG-COOH)) on the morphology and physical properties of chemically crosslinked, semi-interpenetrating polymer network (IPN), chitosan (CHT)/mPEG blend cryosponges. Physicochemical and molecular characterization revealed that the –CHO and –COOH functional groups in mPEG derivatives interacted with the –NH2 functionality of the chitosan chain. The distinguishing feature of the cryosponges was their unique morphological features such as fringe thread-, pebble-, curved quartz crystal-, crystal flower-; and canyon-like structures. The morphological data was well corroborated by the image processing data and physisorption curves corresponding to Type II isotherm with open hysteresis loops. Functionalization of mPEG had no evident influence on the macro-mechanical properties of the cryosponges but increased the matrix strength as determined by the rheomechanical analyses. The cryosponges were able to deliver bioactives (dexamethasone and curcumin) over 10 days, showed varied matrix degradation profiles, and supported neuronal cells on the matrix surface. In addition, in silico simulations confirmed the compatibility and molecular stability of the CHT/mPEG blend compositions. In conclusion, the study confirmed that significant morphological variations may be induced by minimal functionalization and crosslinking of biomaterials.

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Section: Porositometric Evaluation Of the Cht/mpegcryosponges To Devsupporting

confidence: 93%

Macroporous chitosan/methoxypoly(ethylene glycol) based cryosponges with unique morphology for tissue engineering applications

Kumar

Pillay

Choonara

2021

Sci Rep

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“…Ultimately, the crosslinking process can be manipulated to fabricate a precise water absorption ability, water retention and wettability of the skin substitute depending on the requirement of the wound [ 39 ]. For example, high concentration double crosslinking can be utilized in a non-exudating wound where moisture retention plays a big role in the wound healing process.…”

Section: Discussionmentioning

confidence: 99%

Fabrication of Bio-Based Gelatin Sponge for Potential Use as A Functional Acellular Skin Substitute

Arif

Nordin

Hiraoka³

et al. 2020

Polymers

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Gelatin possesses biological properties that resemble native skin and can potentially be fabricated as a skin substitute for full-thickness wound treatment. The native property of gelatin, whereby it is easily melted and degraded at body temperature, could prevent its biofunctionality for various applications. This study aimed to fabricate and characterise buffalo gelatin (Infanca halal certified) crosslinked with chemical type crosslinker (genipin and genipin fortified with EDC) and physicaly crosslink using the dihydrothermal (DHT) method. A porous gelatin sponge (GS) was fabricated by a freeze-drying process followed by a complete crosslinking via chemical—natural and synthetic—or physical intervention using genipin (GNP), 1-ethyl-3-(3-dimethylaminopropyl) (EDC) and dihydrothermal (DHT) methods, respectively. The physicochemical, biomechanical, cellular biocompatibility and cell-biomaterial interaction of GS towards human epidermal keratinocytes (HEK) and dermal fibroblasts (HDF) were evaluated. Results showed that GS had a uniform porous structure with pore size ranging between 60 and 200 µm with high porosity (>78.6 ± 4.1%), high wettability (<72.2 ± 7.0°), high tensile strain (>13.65 ± 1.10%) and 14 h of degradation rate. An increase in the concentration and double-crosslinking approach demonstrated an increment in the crosslinking degree, enzymatic hydrolysis resistance, thermal stability, porosity, wettability and mechanical strength. The GS can be tuned differently from the control by approaching the GS via a different crosslinking strategy. However, a decreasing trend was observed in the pore size, water retention and water absorption ability. Crosslinking with DHT resulted in large pore sizes (85–300 µm) and low water retention (236.9 ± 18.7 g/m2·day) and a comparable swelling ratio with the control (89.6 ± 7.1%). Moreover no changes in the chemical content and amorphous phase identification were observed. The HEK and HDF revealed slight toxicity with double crosslinking. HEK and HDF attachment and proliferation remain similar to each crosslinking approach. Immunogenicity was observed to be higher in the double-crosslinking compared to the single-crosslinking intervention. The fabricated GS demonstrated a dynamic potential to be tailored according to wound types by manipulating the crosslinking intervention.

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“…Gelatin-based scaffolds were utilized as a vehicle for delivery of many bioactive materials and drugs, for example, growth factors, Poly(lactic-co-glycolic acid) (PLGA) and curcumin, ciprofloxacin, and dexamethasone [ 96 , 97 , 98 , 99 ] The existence of growth factors within extracellular matrix imitating scaffolds is substantial for tissue guidance, repair, and development [ 100 ]. Consequently, a spatially and sustained/controlled delivery of growth factors should lead to more effective neo-tissue growth [ 101 ].…”

Section: Pharmaceutical Applicationsmentioning

confidence: 99%

Cosmetic, Biomedical and Pharmaceutical Applications of Fish Gelatin/Hydrolysates

et al. 2021

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There are several reviews that separately cover different aspects of fish gelatin including its preparation, characteristics, modifications, and applications. Its packaging application in food industry is extensively covered but other applications are not covered or covered alongside with those of collagen. This review is comprehensive, specific to fish gelatin/hydrolysate and cites recent research. It covers cosmetic applications, intrinsic activities, and biomedical applications in wound dressing and wound healing, gene therapy, tissue engineering, implants, and bone substitutes. It also covers its pharmaceutical applications including manufacturing of capsules, coating of microparticles/oils, coating of tablets, stabilization of emulsions and drug delivery (microspheres, nanospheres, scaffolds, microneedles, and hydrogels). The main outcomes are that fish gelatin is immunologically safe, protects from the possibility of transmission of bovine spongiform encephalopathy and foot and mouth diseases, has an economic and environmental benefits, and may be suitable for those that practice religious-based food restrictions, i.e., people of Muslim, Jewish and Hindu faiths. It has unique rheological properties, making it more suitable for certain applications than mammalian gelatins. It can be easily modified to enhance its mechanical properties. However, extensive research is still needed to characterize gelatin hydrolysates, elucidate the Structure Activity Relationship (SAR), and formulate them into dosage forms. Additionally, expansion into cosmetic applications and drug delivery is needed.

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3D printed lactose-crosslinked gelatin scaffolds as a drug delivery system for dexamethasone

Cited by 38 publications

References 30 publications

Macroporous chitosan/methoxypoly(ethylene glycol) based cryosponges with unique morphology for tissue engineering applications

Macroporous chitosan/methoxypoly(ethylene glycol) based cryosponges with unique morphology for tissue engineering applications

Fabrication of Bio-Based Gelatin Sponge for Potential Use as A Functional Acellular Skin Substitute

Cosmetic, Biomedical and Pharmaceutical Applications of Fish Gelatin/Hydrolysates

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