3D printing of hydrogel scaffolds for future application in photothermal therapy of breast cancer and tissue repair

Luo, Yongxiang; Wei, Xu; Wan, Yilin; Lin, Xin; Wang, Zhiyong; Huang, Peng

doi:10.1016/j.actbio.2019.05.039

Cited by 104 publications

(66 citation statements)

References 56 publications

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“…After implanting 3D engineered porous scaffolds into mice ears, neovascularization in the implanted scaffolds could be noninvasively monitored and quantified using both AR-PAM and OR-PAM for up to 6 weeks [54]. Polymer porous 3D scaffolds incorporating carbon nanotubes or 3D-printed alginate-polydopamine scaffolds can be used for structural examinations of the 3D scaffolds with PA imaging [55,56]. In these studies, to visualize the network of capillaries (5-10 μm) and 3D engineered porous scaffolds, OR-PAM is used to achieve spatial resolution of 5 μm and axial resolution of 15 μm, but with a limited imaging depth (1 mm).…”

Section: Structural Imaging Of 3d Scaffolds/tissuesmentioning

confidence: 99%

Photoacoustic imaging of cells in a three-dimensional microenvironment

Liu

2020

J Biomed Sci

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Imaging live cells in a three-dimensional (3D) culture system yields more accurate information and spatial visualization of the interplay of cells and the surrounding matrix components compared to using a twodimensional (2D) cell culture system. However, the thickness of 3D cultures results in a high degree of scattering that makes it difficult for the light to penetrate deeply to allow clear optical imaging. Photoacoustic (PA) imaging is a powerful imaging modality that relies on a PA effect generated when light is absorbed by exogenous contrast agents or endogenous molecules in a medium. It combines a high optical contrast with a high acoustic spatiotemporal resolution, allowing the noninvasive visualization of 3D cellular scaffolds at considerable depths with a high resolution and no image distortion. Moreover, advances in targeted contrast agents have also made PA imaging capable of molecular and cellular characterization for use in preclinical personalized diagnostics or PA imaging-guided therapeutics. Here we review the applications and challenges of PA imaging in a 3D cellular microenvironment. Potential future developments of PA imaging in preclinical applications are also discussed.

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Section: Structural Imaging Of 3d Scaffolds/tissuesmentioning

confidence: 99%

Photoacoustic imaging of cells in a three-dimensional microenvironment

Liu

2020

J Biomed Sci

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“…As compared with radiotherapy and chemotherapy, photothermal therapy (PTT) attracted increasing attention because of its highly e cient antitumor, precise spatial-temporal selectivity and no harm to healthy tissues and organs (17)(18)(19). A variety of photothermal agents, such as copper-palladium alloys (19), graphene (20), carbon dots (21), magnetic nanoparticles (22) and Cu7.2S4 Nanoparticles (23) were developed for tumour therapy.…”

Section: Introductionmentioning

confidence: 99%

Graphene-Modified CePO4 Nanorods Effectively Treat Breast Cancer-Induced Bone Metastases and Regulate Macrophage Polarization to Improve Osteo-Inductive Ability

Liu

et al. 2020

Preprint

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Background Breast cancer bone metastasis has become one of the most common complications; however, it may cause cancer recurrence and bone nonunion and local bone defects.Methods Herein, In vitro, we verified the effect of bioscaffold materials on cell proliferation and apoptosis through CCK8 experiment, staining of living dead cells, and flow cytometry. We used immunofluorescence technology and flow cytometry to verified whether bioscaffold materials regulated the polarization of macrophages, and we use ALP staining, alizarin red staining and PCR to verify whether the bioscaffold material promotes bone regeneration. In vivo, we once again studied the effect of bioscaffold materials on tumors by measuring tumor volume in mice, Tunel staining, and caspase-3 immunofluorescence. We also constructed a mouse skull ultimate defect model to verify the effect on bone regeneration.Results Graphene oxide (GO) nanoparticles, hydrated CePO4 nanorods and bioactive chitosan (CS) are combined to form a bioactive multifunctional CePO4/CS/GO scaffolds, which has the following characteristics such as photothermal therapy to kill tumors, macrophage polarization promotes blood vessel formation and induces bone formation. The CePO4/CS/GO scaffold activates the caspase-3 proteasein local tumor cells, thereby lysing DNA between nucleosomes and causing apoptosis. On the one hand, the as-released Ce3+ ions promote the M2 polarization of macrophages, which secretes vascular endothelial growth factor (VEGF) and Arginase-1 (Arg-1), which promotes angiogenesis. On the other hand, the as-released Ce3+ ions also activated BMP-2/Smad signaling pathway that facilitated bone tissue regeneration.Conclusion the multifunctional CePO4/CS/GO scaffolds may become a promising platform for therapy of breast cancer bone metastases.

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“…13 Studies with alginate hydrogels loaded with polydopamine and element-doped bioactive ceramic glass scaffolds have also exhibited successful in vivo destruction of breast and bone cancers, respectively. 14,15 More recently, focal therapy methods have been coupled with biomaterial scaffolds which have previously been used to recruit metastatic cancer cells. Non-invasive hyperthermia treatment of infiltrated cells was successfully completed in vivo using porous PCL scaffolds with an embedded aluminum disk under an alternating magnetic field.…”

Section: Introductionmentioning

confidence: 99%

“…In vitro killing of HeLa cervical cancer cells has been shown using near infrared (NIR) laser irradiation of gelatin and gold nanoparticle composite scaffolds 13 . Studies with alginate hydrogels loaded with polydopamine and element‐doped bioactive ceramic glass scaffolds have also exhibited successful in vivo destruction of breast and bone cancers, respectively 14,15 . More recently, focal therapy methods have been coupled with biomaterial scaffolds which have previously been used to recruit metastatic cancer cells.…”

Section: Introductionmentioning

confidence: 99%

Iron oxide‐loaded polymer scaffolds for non‐invasive hyperthermic treatment of infiltrated cells

et al. 2020

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Focal therapies such as hyperthermia have been successfully used to treat solid localized tumors; however, they are not easily applied to cancers that may present in a disseminated form such as ovarian cancer. To address this need, iron oxide (IO) particles were incorporated into microporous poly(caprolactone) scaffolds previously shown to recruit disseminating cancer cells. Under an alternating magnetic field, IO-loaded scaffolds exhibited heating and killed ID8 ovarian cancer cells in vitro. After implantation in the intraperitoneal cavity of mice, IO-loaded scaffolds became infiltrated with tissue after 6-7 weeks, and infiltrated cells were successfully treated ex vivo. Finally, IO-loaded scaffolds noninvasively killed infiltrated cells in vivo as evidenced by decreases in number of nuclei. These studies demonstrate the promising use of IO-loaded scaffolds as a tool for noninvasive hyperthermia, which could be an innovative modality for treatment of disseminated cancers.

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3D printing of hydrogel scaffolds for future application in photothermal therapy of breast cancer and tissue repair

Cited by 104 publications

References 56 publications

Photoacoustic imaging of cells in a three-dimensional microenvironment

Photoacoustic imaging of cells in a three-dimensional microenvironment

Graphene-Modified CePO4 Nanorods Effectively Treat Breast Cancer-Induced Bone Metastases and Regulate Macrophage Polarization to Improve Osteo-Inductive Ability

Iron oxide‐loaded polymer scaffolds for non‐invasive hyperthermic treatment of infiltrated cells

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