3D Printing of Personalized Artificial Bone Scaffolds

Jariwala, Shailly H.; Lewis, Gregory S.; Bushman, Zachary J.; Adair, James H.; Donahue, Henry J.

doi:10.1089/3dp.2015.0001

Cited by 135 publications

(88 citation statements)

References 47 publications

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“…Further, it is also possible to produce complex shapes with the 3D nanofiber aerogels capable of fitting bone defects using a mold generated by 3D printing technique based on the 3D imaging of bone defects. [35] However, the 3D hybrid aerogels may need further toughening in order to applied for tissue engineering of load bearing long bones.…”

Section: Discussionmentioning

confidence: 99%

Novel 3D Hybrid Nanofiber Aerogels Coupled with BMP‐2 Peptides for Cranial Bone Regeneration

Weng

Boda

Wang

et al. 2018

Adv Healthcare Materials

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An ideal synthetic bone graft is a combination of the porous and nanofibrous structure presented by natural bone tissue as well as osteoinductive biochemical factors such as bone morphogenetic protein 2 (BMP-2). In this work, ultralight 3D hybrid nanofiber aerogels composed of electrospun PLGA-collagen-gelatin and Sr-Cu codoped bioactive glass fibers with incorporation of heptaglutamate E7 domain specific BMP-2 peptides have been developed and evaluated for their potential in cranial bone defect healing. The nanofiber aerogels are surgically implanted into 8 mm × 1 mm (diameter × thickness) critical-sized defects created in rat calvariae. A sustained release of E7-BMP-2 peptide from the degradable hybrid aerogels significantly enhances bone healing and defect closure over 8 weeks in comparison to unfilled defects. Histomorphometry and X-ray microcomputed tomography (µ-CT) analysis reveal greater bone volume and bone formation area in case of the E7-BMP-2 peptide loaded hybrid nanofiber aerogels. Further, histopathology data divulged a near complete nanofiber aerogel degradation along with enhanced vascularization of the regenerated tissue. Together, this study for the first time demonstrates the fabrication of 3D hybrid nanofiber aerogels from 2D electrospun fibers and their loading with therapeutic osteoinductive BMP-2 mimicking peptide for cranial bone tissue regeneration.

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Section: Discussionmentioning

confidence: 99%

Novel 3D Hybrid Nanofiber Aerogels Coupled with BMP‐2 Peptides for Cranial Bone Regeneration

Weng

Boda

Wang

et al. 2018

Adv Healthcare Materials

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“…As an outstanding example, fracture, nonunion and infection are some complicated drawbacks of more than 30% of the allograft procedures and also requiring high volume of bone in autograft method (DE LONG et al, 2007;Doyle et al, 2015). Therefore, researchers have gone toward preparation of suitable structural frameworks named bone scaffold to provide a support for cells (Jariwala et al, 2015;Poh, 2014). Such structures should have appropriate geometrical and mechanical properties in order to provide suitable bone treatment (Giannitelli et al, 2014;Sanz-Herrera et al, 2008;Yang et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Numerical investigation of the mechanical properties of the additive manufactured bone scaffolds fabricated by FDM: The effect of layer penetration and post-heating

Naghieh

Karamooz-Ravari

Badrossamay

et al. 2016

Journal of the Mechanical Behavior of Biomedical Materials

127

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In recent years, thanks to additive manufacturing technology, researchers have gone towards the optimization of bone scaffolds for the bone reconstruction. Bone scaffolds should have appropriate biological as well as mechanical properties in order to play a decisive role in bone healing. Since the fabrication of scaffolds is time consuming and expensive, numerical methods are often utilized to simulate their mechanical properties in order to find a nearly optimum one. Finite element analysis is one of the most common numerical methods that is used in this regard. In this paper, a parametric finite element model is developed to assess the effects of layer adhesion on the mechanical properties of bone scaffolds. To be able to validate this model, some compression test specimens as well as bone scaffolds are fabricated with biocompatible and biodegradable poly lactic acid using fused deposition modeling. All these specimens are tested in compression and their elastic modulus is obtained. Using the material parameters of the compression test specimens, the finite element analysis of the bone scaffold is performed. The obtained elastic modulus is compared with experiment indicating a good agreement. Accordingly, the proposed finite element model is able to predict the mechanical behavior of fabricated bone scaffolds accurately. In addition, the effect of post-heating of bone scaffolds on their elastic modulus is investigated. The results demonstrate that the numerically predicted elastic modulus of scaffold is closer to experimental outcomes in comparison with as-build samples.

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“…Here, for the first time, we have attempted to draw knowledge from different spheres of cell engineering (like nonviral gene delivery; Olton et al, ; Santos et al, ) with the purpose of coaxing the scientific community towards merging these advanced techniques with bone bioprinting. Historical progress of this field was heavily dependent upon cell‐free printing using hard bone‐like materials like ceramics (HA/β‐tricalcium phosphate), bioglass‐ceramic composites and polymers (PCL), which will not be discussed here as many good reviews have already covered those aspects (Bose, Vahabzadeh, & Bandyopadhyay, ; Jariwala et al, ; Li, Chen, Fan, & Zhou, ; Wen et al, ). A major limitation is that such ceramic‐based scaffolds (some of which have been listed in Figure ) require post‐fabrication processing such as high temperature sintering or dissolution in organic solvents (Almela et al, ; Roohani‐Esfahani, Newman, & Zreiqat, ) to confer stability on constructs and hence do not support live‐cell printing.…”

Section: Introductionmentioning

confidence: 99%

Advances in three‐dimensional bioprinting of bone: Progress and challenges

Midha

Dalela

Sybil

et al. 2019

J Tissue Eng Regen Med

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Several attempts have been made to engineer a viable three‐dimensional (3D) bone tissue equivalent using conventional tissue engineering strategies, but with limited clinical success. Using 3D bioprinting technology, scientists have developed functional prototypes of clinically relevant and mechanically robust bone with a functional bone marrow. Although the field is in its infancy, it has shown immense potential in the field of bone tissue engineering by re‐establishing the 3D dynamic micro‐environment of the native bone. Inspite of their in vitro success, maintaining the viability and differentiation potential of such cell‐laden constructs overtime, and their subsequent preclinical testing in terms of stability, mechanical loading, immune responses, and osseointegrative potential still needs to be explored. Progress is slow due to several challenges such as but not limited to the choice of ink used for cell encapsulation, optimal cell source, bioprinting method suitable for replicating the heterogeneous tissues and organs, and so on. Here, we summarize the recent advancements in bioprinting of bone, their limitations, challenges, and strategies for future improvisations. The generated knowledge will provide deep insights on our current understanding of the cellular interactions with the hydrogel matrices and help to unravel new methodologies for facilitating precisely regulated stem cell behaviour.

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3D Printing of Personalized Artificial Bone Scaffolds

Abstract: Additive manufacturing technologies, including three -dimensional printing (3DP),

Cited by 135 publications

References 47 publications

Novel 3D Hybrid Nanofiber Aerogels Coupled with BMP‐2 Peptides for Cranial Bone Regeneration

Novel 3D Hybrid Nanofiber Aerogels Coupled with BMP‐2 Peptides for Cranial Bone Regeneration

Numerical investigation of the mechanical properties of the additive manufactured bone scaffolds fabricated by FDM: The effect of layer penetration and post-heating

Advances in three‐dimensional bioprinting of bone: Progress and challenges

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