3D structure modeling of complexes formed by CrmB TNF-binding proteins of Variola and cowpox viruses with murine and human TNFs

TNF-Binding domain of the variola virus CrmB protein synthesized in Escherichia coli cells effectively interacts with human TNF

“…This was also clearly confirmed by successful treatment of these pathologies using targeted anti TNF therapy [1].…”

Induction of TNF-α Production by Metal Complexes of γ-Globulin Fraction Proteins and Copper and Zinc Cations

“…The biological signifi cance of a certain level of TNF-α induction, as well as the suppression of this process by the basal mechanisms of cell function regulation, is related to the prevention of autoreactivity due to a forced synthesis of TNF-α and IFN- [1]. This process probably contributes to antiviral activity of cells, which is associated with a synergistic effect of some combinations of TNF-α and IFN.…”

Biological Effects of Individually Synthesized TNF-Binding Domain of Variola Virus CrmB Protein

“…A new type of cytokine inhibitor blocking activity of TNF has been isolated and characterized: variola virus TNF-binding protein (VARVCrmB) [3,4]. This protein neutralizes TNF activity in vitro and in vivo [1,2,5]. In our experiments VARVCrmB has inhibited TNF-induced oxidative metabolic and cytokine-producing activities of mouse blood leukocytes [7] and of donor mononuclear cells [8].…”

“…Removal of the C-terminal chemokine-binding domain from VARV-CrmB protein was inessential for the effi ciency of its inhibition of TNF cytotoxicity towards L929 mouse fi broblast culture and for TNF-induced oxidative metabolic activity of mouse blood leukocytes. The results of this study could form the basis for further studies of VARV-TNF-BP mechanisms of activity for prospective use in practical medicine.New data on the creation and practical use of drugs effectively inhibiting the synthesis and processing of cytokines, playing the key role in the pathogenesis of many socially signifi cant diseases, have been published over the latest decade [5]. These drugs are most often based on monoclonal antibodies [13,15] or recombinant chimeric proteins consisting of receptor and immunoglobulin domains [10].…”

“…New data on the creation and practical use of drugs effectively inhibiting the synthesis and processing of cytokines, playing the key role in the pathogenesis of many socially signifi cant diseases, have been published over the latest decade [5]. These drugs are most often based on monoclonal antibodies [13,15] or recombinant chimeric proteins consisting of receptor and immunoglobulin domains [10].…”