4-(N,N-Dialkylthiocarbamoylthio)-5-nitropyrimidines as new potential nitric oxide donors

Ryabova, O. B.; Khmel'nitskaya, E. Yu.; Makarov, Vadim; Алексеева, Л. М.; Grigor'ev, N. B.; Граник, В. Г.

doi:10.1007/s11172-006-0203-5

Cited by 5 publications

(2 citation statements)

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“…The nitric oxide (NO) donor, 6-methoxy-5-nitropyrimidin-4-yl pyrrolidine-1-carbodithioate (20), at a final concentration of 100 μM was used to assess the effect of nitrosative stress on M. tuberculosis viability. For starvation of M. tuberculosis , cultures were washed in phosphate buffered saline (PBS) twice before being resuspended in PBS.…”

Section: Methodsmentioning

confidence: 99%

Cmr is a redox-responsive regulator of DosR that contributes to M. tuberculosis virulence

Smith

Bochkareva

Rolfe

et al. 2017

Nucleic Acids Research

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Mycobacterium tuberculosis (MTb) is the causative agent of pulmonary tuberculosis (TB). MTb colonizes the human lung, often entering a non-replicating state before progressing to life-threatening active infections. Transcriptional reprogramming is essential for TB pathogenesis. In vitro, Cmr (a member of the CRP/FNR super-family of transcription regulators) bound at a single DNA site to act as a dual regulator of cmr transcription and an activator of the divergent rv1676 gene. Transcriptional profiling and DNA-binding assays suggested that Cmr directly represses dosR expression. The DosR regulon is thought to be involved in establishing latent tuberculosis infections in response to hypoxia and nitric oxide. Accordingly, DNA-binding by Cmr was severely impaired by nitrosation. A cmr mutant was better able to survive a nitrosative stress challenge but was attenuated in a mouse aerosol infection model. The complemented mutant exhibited a ∼2-fold increase in cmr expression, which led to increased sensitivity to nitrosative stress. This, and the inability to restore wild-type behaviour in the infection model, suggests that precise regulation of the cmr locus, which is associated with Region of Difference 150 in hypervirulent Beijing strains of Mtb, is important for TB pathogenesis.

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Section: Methodsmentioning

confidence: 99%

Cmr is a redox-responsive regulator of DosR that contributes to M. tuberculosis virulence

Smith

Bochkareva

Rolfe

et al. 2017

Nucleic Acids Research

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“…Scheme 49 shows the pathway of subse quent transformations of this alkaloid through thebaine (75) and codeine (55) into morphine (54). The formation of salutaridine (in liver and plant microsomes) assumes the involvement of reticuline (76).…”

Section: Scheme 48mentioning

confidence: 99%

Biotransformations of drugs belonging to nitrogen heterocycles

Граник

2010

Russ Chem Bull

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The present review covers general aspects related to the metabolism of organic compounds, primarily, of biologically active azaheterocycles used as drugs. Compounds of this type occupy a dominant place among known drugs. Data on the main chemical processes determining the pathways of principal biotransformations, such as redox reactions, hydrolysis, alkylation, acy lation, various conjugations, isomerizations, and condensations, are summarized. The metabo lisms of heterocycles is considered based on the ring size of the substrate.

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