5′ distal and proximal <i>cis</i>‐acting regulator elements are required for developmental control of a rice seed storage protein <i>glutelin</i> gene

Zheng, Zhenwei; Kawagoe, Yasuhiro; Xiao, Shou‐Hua; Z, Li; Okita, Thomas W.; Hau, Toan Lam; Lin, Angy; Murai, Norimoto

doi:10.1046/j.1365-313x.1993.04020357.x

Cited by 94 publications

(58 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The potent modified CaMV 35S promoter from pRTL2, which contains double enhancer elements and a TEV leader, was used to drive GUS-MS6X-prolamine RNA expression in BY-2 cells, whereas the rice seed-specific prolamine Prol promoter was used to drive the expression of this gene in developing rice endosperm cells. In our hands, the Prol promoter was found to be a relatively weak promoter (our unpublished observations), especially compared with the Gt1 promoter, which is one of the strongest rice seed-specific promoters available (Zheng et al, 1993). Increased constitutive expression has been suggested to account for the formation of a single large particle containing ASH1 RNA instead of the several smaller particles evident under controlled expression (Bertrand et al, 1998).…”

Section: Discussionmentioning

confidence: 73%

“…To visualize prolamine RNA movement in developing rice endosperm cells, the promoters from the rice glutelin Gt1 (Zheng et al, 1993) and prolamine pProl (Wu et al, 1998) were inserted upstream of NLS-MS2-GFP and GUS-MS6X-prolamine, respectively (Figure 1). These two genes then were cloned into the T-DNA vector pCAMBIA1301, which was used to transform rice via Agro-bacterium tumefaciens .…”

Section: Prolamine Rnas Form Particles In Developing Rice Endospermmentioning

confidence: 99%

“…To combine NLS-MS2 and GFP-Tagp, a NotI-XhoI fragment containing GFP-Tagp was cloned into the same sites of pSL1180 containing the NLS-MS2 sequence. A BamHI-BglII fragment containing NLS-MS2-GFP-Tagp then was transferred into the BglII site of pSP72, which contains the 1.8-kb Gt1 promoter fragment (Zheng et al, 1993) inserted at the EcoRI-BglII sites (Choi et al, 2000).…”

Section: Plasmid Dna Construction and Plant Transformationmentioning

confidence: 99%

See 2 more Smart Citations

The Transport of Prolamine RNAs to Prolamine Protein Bodies in Living Rice Endosperm Cells[W]

et al. 2003

View full text Add to dashboard Cite

RNAs that code for the major rice storage proteins are localized to specific subdomains of the cortical endoplasmic reticulum (ER) in developing endosperm. Prolamine RNAs are localized to the ER and delimit the prolamine intracisternal inclusion granules (PB-ER), whereas glutelin RNAs are targeted to the cisternal ER. To study the transport of prolamine RNAs to the surface of the prolamine protein bodies in living endosperm cells, we adapted a two-gene system consisting of green fluorescent protein (GFP) fused to the viral RNA binding protein MS2 and a hybrid prolamine RNA containing tandem MS2 RNA binding sites. Using laser scanning confocal microscopy, we show that the GFP-labeled prolamine RNAs are transported as particles that move at an average speed of 0.3 to 0.4 m/s. These prolamine RNA transport particles generally move unidirectionally in a stop-and-go manner, although nonlinear bidirectional, restricted, and nearly random movement patterns also were observed. Transport is dependent on intact microfilaments, because particle movement is inhibited rapidly by the actin filament-disrupting drugs cytochalasin D and latrunculin B. Direct evidence was obtained that these prolamine RNA-containing particles are transported to the prolamine protein bodies. The significance of these results with regard to protein synthesis in plants is discussed.

show abstract

Section: Discussionmentioning

confidence: 73%

Section: Prolamine Rnas Form Particles In Developing Rice Endospermmentioning

confidence: 99%

Section: Plasmid Dna Construction and Plant Transformationmentioning

confidence: 99%

See 1 more Smart Citation

The Transport of Prolamine RNAs to Prolamine Protein Bodies in Living Rice Endosperm Cells[W]

et al. 2003

View full text Add to dashboard Cite

show abstract

“…A dissection of the GluB-1 promoter has shown that the GCN4 and AACA motifs are important for driving high-level gene expression in tobacco endosperms . Analysis of the GluA-2 (Gt1) promoter in homologous rice plants has identified one distal and six proximal ciselements, including GCN4 and AACA, that are implicated in the developmental control of GluA-2 expression (Zheng et al, 1993). Taken together, these studies imply that the GCN4 and AACA motifs are probably common functional cis-elements required for regulating endosperm-specific expression of rice glutelin genes.…”

Section: Introductionmentioning

confidence: 98%

The GCN4 motif in a rice glutelin gene is essential for endosperm‐specific gene expression and is activated by Opaque‐2 in transgenic rice plants

et al. 1998

View full text Add to dashboard Cite

SummaryThe GCN4 motif is conserved in a number of seed storage protein genes, and promoter fragments containing this motif have been shown to be involved in controlling seed-specific expression of the genes studied. All genes encoding the rice seed storage protein glutelin contain the GCN4 motif at similar sites in their 5Ј flanking regions. Using a stable homologous transgenic system, we have analysed the promoter of the rice glutelin gene GluB-1 and demonstrated that the GCN4 motif functions as an essential cis-element for endosperm-specific gene expression. Moreover, a 21 bp GluB-1 promoter fragment spanning the GCN4 motif, as a multimer, directed GUS gene expression in endosperm of transgenic rice plants, when fused directly to the core promoter (-46) of CaMV 35S. In transiently transfected rice protoplasts, over a hundredfold transactivation was observed from the 21 bp sequence by the bZIP type transcriptional activator Opaque-2 (O2) co-expressed under a CaMV 35S promoter. The transactivation was also evident in transgenic plants containing both O2 and the 21 bp sequence/GUS fusion. The O2-mediated activation requires binding of O2 to an intact GCN4 motif. Our results suggest that a bZIP protein functionally similar to O2 may exist in rice and participate in controlling the endosperm-specific expression of GluB-1 through the GCN4 motif.

show abstract

“…This is especially true in the case of monocot plants, where many of the promoter analyses of cereal storage protein genes have carried out by transient assays using particle bombardment or heterologous transgenic tobacco system (4 -6). Dissection analyses of promoter using homologous stable transgenic plant have been carried out only on glutelin genes of the rice (7)(8)(9). Endosperm-specific expression of cereal storage protein genes is regulated by the combinatorial interactions of several cis-elements (3).…”

mentioning

confidence: 99%

A Rice Functional Transcriptional Activator, RISBZ1, Responsible for Endosperm-specific Expression of Storage Protein Genes through GCN4 Motif

Onodera¹,

Suzuki²,

Wu³

et al. 2001

Journal of Biological Chemistry

152

149

View full text Add to dashboard Cite

The GCN4 motif, a cis-element that is highly conserved in the promoters of cereal seed storage protein genes, plays a central role in controlling endospermspecific expression. This motif is the recognition site for a basic leucine zipper transcriptional factor that belongs to the group of maize Opaque-2 (O2)-like proteins. Five different basic leucine zipper cDNA clones, designated RISBZ1-5, have been isolated from a rice seed cDNA library. The predicted gene products can be divided into two groups based on their amino acid sequences. Although all the RISBZ proteins are able to interact with the GCN4 motif, only RISBZ1 is capable of activating (more than 100-fold expression) the expression of a reporter gene under a minimal promoter fused to a pentamer of the GCN4 motif. Loss-of-function and gain-of-function experiments using the yeast GAL4 DNA binding domain revealed that the proline-rich N-terminal domain (27 amino acids in length) is responsible for transactivation. The RISBZ1 protein is capable of forming homodimers as well as heterodimers with other RISBZ subunit proteins. RISBZ1 gene expression is restricted to the seed, where it precedes the expression of storage protein genes. When the RISBZ1 promoter was transcriptionally fused to the ␤-glucuronidase reporter gene and the chimeric gene was introduced into rice, the ␤-glucuronidase gene is specifically expressed in aleurone and subaleurone layer of the developing endosperm. These findings suggest that the specific expression of transcriptional activator RISBZ1 gene may determine the endosperm specificity of the storage protein genes.Regulated gene expression is mediated by the combinatorial interactions of multiple cis-elements in the gene's promoter. Specific binding of transcriptional factors to the cognate ciselements constitute a crucial step in transcription initiation and, in turn, on the spatial and temporal expression of genes.Seed storage protein genes provide a model system for the study on the regulatory mechanisms of plant genes (1), since their expression is restricted to a specific tissue and stage during seed development. These specific temporal and spatial expression patterns may be explained as the result of regulatory assemblies of several transcriptional activators that recognize the cis-elements implicated in seed-specific expression. Therefore, to understand such molecular mechanisms, characterization of cis-elements and transcription factors has been performed on many storage protein genes of several crop plants (2,3). Despite numerous studies, the mechanism by which these genes are regulated are poorly understood, since many of the essential cis-elements have not been identified. This is especially true in the case of monocot plants, where many of the promoter analyses of cereal storage protein genes have carried out by transient assays using particle bombardment or heterologous transgenic tobacco system (4 -6). Dissection analyses of promoter using homologous stable transgenic plant have been carried out only on glutelin genes of ...

show abstract

5′ distal and proximal cis‐acting regulator elements are required for developmental control of a rice seed storage protein glutelin gene

Cited by 94 publications

References 0 publications

The Transport of Prolamine RNAs to Prolamine Protein Bodies in Living Rice Endosperm Cells[W]

The Transport of Prolamine RNAs to Prolamine Protein Bodies in Living Rice Endosperm Cells[W]

The GCN4 motif in a rice glutelin gene is essential for endosperm‐specific gene expression and is activated by Opaque‐2 in transgenic rice plants

A Rice Functional Transcriptional Activator, RISBZ1, Responsible for Endosperm-specific Expression of Storage Protein Genes through GCN4 Motif

Contact Info

Product

Resources

About