5′ ins/del and 3′ VNTR polymorphisms in the apolipoprotein B gene in relation to lipids and coronary artery disease

Raîs, L.; Omezzine, Asma; Kchok, Kaouther; Belkahla, Radhia; HadjMbarek, Ibtihel Ben; Rejeb, Jaleleddine Ben; Rejeb, N.; Nabli, Naoufel; Bibi, Amina; Massoud, Taieb; Abdelaziz, Ahmed; Boughzala, E.; Bouslama, Ali

doi:10.1515/cclm.2008.067

Cited by 13 publications

(15 citation statements)

References 22 publications

(31 reference statements)

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“…M/L carriers had TC, LDL-C, and apoB concentrations significantly higher than those of S/S, S/M, S/L, or M/M carriers, and individuals with the L allele accordingly exhibited TC, LDL-C, and apoB levels significantly higher than those with M or S alleles. These results were consistent with those of other investigators (Hansen et al, 1993;Pan et al, 1998;Rebhi et al, 2008). However, in children with PNS, no significant differences in serum lipid levels were observed for variant genotypes and alleles of the apoB 3' VNTR.…”

Section: Discussionsupporting

confidence: 93%

Serum lipid abnormalities are not associated with apoB 3' VNTR polymorphism in nephrotic children

Hu²,

Qin³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. Apolipoprotein B (apoB) gene 3' variable number of tandem repeat (VNTR) is highly variable, and therefore can be an informative marker for associative analysis of lipid metabolism. This is the first report focusing on a possible association of apoB VNTR polymorphism with nephrotic hyperlipidemia. Genomic DNA was extracted from 500 children with primary nephrotic syndrome (PNS) and 500 healthy controls. The apoB genotype was determined by PCR analysis. Allele size distribution followed a unimodal curve, with the main peak at the hypervariable element 35 (HVE35); the most prevalent genotype was HVE35/35 in both control and PNS children. The genotype and allele distributions of apoB variants in PNS children were not significantly different from controls. There was significant variation in serum lipid profiles among different genotypes in control children. Individuals with the long (L) allele exhibited significantly higher total cholesterol, low-density lipoprotein cholesterol (LDL-C) and apoB levels than those with the medium (M) or short (S) allele; consequently, M/L carriers had significantly higher total cholesterol, LDL-C and apoB concentrations than did S/S, S/M, S/L, or M/M carriers. However, in PNS children, no significant differences in serum lipid levels were observed among individuals with different genotypes and alleles of apoB 3' VNTR. We conclude that hyperlipidemia in nephrotic children is not associated with apoB 3' VNTR polymorphism.

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Section: Discussionsupporting

confidence: 93%

Serum lipid abnormalities are not associated with apoB 3' VNTR polymorphism in nephrotic children

Hu²,

Qin³

et al. 2013

Genet. Mol. Res.

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“…In this study we did not measure APOB level in serum to correlate with 3ʹ-VNTR repeat numbers in diabetic and non-diabetic individuals. Earlier studies have reported high APOB protein level in serum of individuals with larger APOB 3ʹ-VNTR alleles 18,36,37 . In Canadian aboriginal people, high level of plasma APOB protein was found to be associated with type 2 diabetes as well as a better predictor of risk compared to LDL or HDL cholesterol 12 .…”

Section: Discussionmentioning

confidence: 88%

“…Alleles with higher repeats (≥36 repeats) were shown to be strongly correlated with coronary heart disease 18,[33][34][35] . APOB 3ʹ-VNTR alleles with higher repeats are also significantly associated with higher levels of total cholesterol (TC) and low density lipoprotein-cholesterol (LDL-C) 36,37 . Kanani et al detected raised APOB protein in 56.7% of the type 2 diabetics 7 .…”

Section: Discussionmentioning

confidence: 99%

Association of APOB 3ʹ-VNTR alleles with type 2 diabetes, BMI, systolic and diastolic blood pressure

Sajib

Khan

Haque

et al. 2018

Bangladesh J Med Sci

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Background: Apolipoprotein B (APOB) is a component of chylomicrons, low-density lipoproteins (LDL), very low-density lipoproteins (VLDL), intermediate density lipoproteins (IDL) and functions as the main protein for transporting cholesterol to peripheral cells. APOB gene has an AT-rich VNTR site at its 3ʹ-untranslated region (3ʹ-UTR). APOB 3ʹ-VNTR alleles with ≥36 repeats have been shown to be strongly associated with increased serum lipid levels, gallstone formation and coronary artery disease. Objectives: To investigate any possible association of APOB 3ʹ-VNTR alleles with type 2 diabetes, BMI, systolic and diastolic blood pressure. Materials and methods: APOB 3ʹ-VNTR region in the DNA of non-diabetic controls and type 2 diabetic patients were amplified by polymerase chain reaction (PCR) and the numbers of core repeat in the amplified products were determined. Frequencies of the APOB alleles and genotypes among the controls and the patients were calculated and statistical analyses were performed. Results and discussion: Here we report for the first time that APOB 3ʹ-VNTR alleles have different distribution frequencies among type 2 diabetic and non-diabetic individuals. We also observed higher body mass index (BMI), systolic and diastolic blood pressures in individuals who had at least one APOB 3ʹ-VNTR allele with ≥35 repeats. Conclusion: Our study might bridge among the genetic signature of APOB 3ʹ-VNTR, high APOB protein level in blood, diabetes and other co-morbidities.

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“…The main characteristics of the included studies are listed in Table (1a, 1b, 1c, 1d, in Supplementary document). A total of 47 eligible studies (Aburatani et al 1987;Al-Bustan et al 2009;Anghebem-Oliveira et al 2009;Beneš et al 2000a, b;Berkinbayev et al 2014;Bohn et al 1993Bohn et al , 1994Buraczynska et al 2000;Corbo et al 1997;De Lorenzo et al 1993;de Padua Mansur et al 2000;Duman et al 2005;Dunning et al 1993;Ferns and Galton 1986;Gallegos-Arreola et al 2012;Gardemann et al 1998;Genest et al 1990;Hegele et al 1986;Hong et al 2001;Hosking et al 1992;Huang et al 2012;Kallel et al 2008;Machado et al 2001;Marshall et al 1994;Mendis et al 1991;Miettinen et al 1994;Myant et al 1989;Nieminen et al 1992;Pan et al 1995;Paulweber et al 1990;Rebhi et al 2008;Saha et al 1992;Salazar et al 2000;Scartezini et al 2003;Stepanov et al 1998;Tybjaerg-Hansen et al 1991;Visvikis et al 1993;Wiklund et al 1989;Wu et al 1994;Xu and Zhou 1998;…”

Section: Characteristics Of the Eligible Studiesmentioning

confidence: 99%

Four Apolipoprotein B gene polymorphisms and the risk for coronary artery disease: a meta-analysis of 47 studies

et al. 2015

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Apolipoprotein B plays a central role in lipoprotein metabolism. Many studies have evaluated the association between Apolipoprotein B gene polymorphisms (XbaI, EcoRI, SpIns/Del, MspI) and the risk for coronary artery disease and myocardial infarction. However, the results remain inconsistent, particularly among different populations. To more precisely determine the association between Apolipoprotein B gene polymorphisms and coronary artery disease/myocardial infarction risk, we performed a meta-analysis via a comprehensive search of electronic databases (up to February 1st, 2015), odds ratios (OR) and 95 % confidence intervals were calculated using a fixed or random effect model. A total of 47 studies, with 9411 coronary artery disease/myocardial infarction cases and 9082 controls, were included in this meta-analysis. The combined results revealed significant associations between an increased risk of coronary artery disease/myocardial infarction and EcoRI (AA vs GG: OR 1.511, 95 % confidence interval (CI) 1.098, 2.078) and SpIns/Del (DD vs II: OR 1.331, 95 % CI 1.064, 1.665) alleles in the general population. In a subgroup analysis stratified by ethnicity, the T allele of the XbaI variant was associated with a decreased risk in Caucasians, whereas it was associated with an increased risk among the East Asian population. No significant correlation was detected between the A allele of the MspI variant and the coronary artery disease/myocardial infarction risk in either the general population or any ethnic subgroup. The results of our study suggest that Apolipoprotein B gene polymorphisms may affect the coronary artery disease/myocardial infarction susceptibility and these effects may display notable discrepancies among different populations.Keywords Apolipoprotein B Á Polymorphism Á Coronary artery disease Á Myocardial infarction Á Meta-analysis Electronic supplementary material The online version of this article (

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5′ ins/del and 3′ VNTR polymorphisms in the apolipoprotein B gene in relation to lipids and coronary artery disease

Abstract: The two polymorphisms exert an impact on lipid levels and contribute to the susceptibility to the development of CAD.

Cited by 13 publications

References 22 publications

Serum lipid abnormalities are not associated with apoB 3' VNTR polymorphism in nephrotic children

Serum lipid abnormalities are not associated with apoB 3' VNTR polymorphism in nephrotic children

Association of APOB 3ʹ-VNTR alleles with type 2 diabetes, BMI, systolic and diastolic blood pressure

Four Apolipoprotein B gene polymorphisms and the risk for coronary artery disease: a meta-analysis of 47 studies

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