[6] Characterization of sialic acids

Adhesion of K99 fimbriated Escherichia coli to pig intestinal epithelium: correlation of adhesive and non-adhesive phenotypes with the sialoglycolipid content

“…NGlycoloyl-and N-acetylneuraminic acids were separated by HPTLC on cellulose using butan-1-ol/propan-1-ol/HCl (1 :2 : 1, by vol.) (Schauer, 1978). NeuAc and NeuGc (Sigma) served as reference compounds.…”

Changes in the glycoprotein composition of plasma membrane during the differentiation of friend erythroleukemia cells

“…Sialic acid isolation and analysis were performed by standard techniques [20]. Briefly, a plasma membrane fraction of cells labelled with [3H]ManNAc was subjected to mild acid hydrolysis (pH 2-3, 60 min at 60°C) followed by extensive dialysis.…”

“…Hydrolysis was repeated in 0.1 M HC1 for 60 min at 80°C and dialysis was performed accordingly. The dialysates were combined and purified by ion-exchange chromatography [20]. Sialic acids were analyzed by thin-layer chromatography on 0.2 mm cellulose plastic sheets (Merck) using the solvent system n-butanol/n-propanol/0.1 M HC1 (1 : 2 : 1, v/v).…”

Short Communication Report: Sialic acid and sialidase profiles of the mosquito <i>Culex quinquefasciatus</i> say 1823 in Samaru, Zaria, Nigeria

“…Free sialic acid concentration was determined by the Thiobarbituric Acid Method according to Schauer (1978). The process was carried out by oxidizing 500 µl of the hydrolyzed sample in 250 µl of peroxidate solution and incubated in a water bath at 37 0 C for 30 min.…”

“…100 µl of Sodium arsenite was added to the mixed solution to neutralize the excess peroxidate solution, after which 1000 µl of Thiobarbituric Acid was added and heated in a water bath at 100 0 C for 8 min to form a coloured complex, at which point, 2500 µl of Butanol Acetic Acid (19:1) was added to the solution to extract the sialic acid. The solution was centrifuged at 5 rpm for ten minutes and optical readings at 549 nm in the spectrophometer and recorded, the actual concentrationsof sialic acid was extrapolated from the standard curve (Schauer 1978;Sallau et al 2001).…”