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Bocharova, E. N.; Abdumalikov, R. A.; Брагина, Е. Е.; Климова, Р. Р.; Adueva, S M; Medzhidova, M G; Kurilo, L. F.; Кущ, А А

doi:10.1023/a:1025179307236

Cited by 8 publications

(3 citation statements)

References 9 publications

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“…One of the possible explanations for such a difference is abortive HCMV infection of spermatozoa. This suggestion is supported by the fact that only empty capsids without electron-dense core (type A and type B) have been detected in mature sperm cells [24]. Our group and other researchers failed to identify filled capsids (type C) and enveloped virions into spermatozoa.…”

Section: Discussionsupporting

confidence: 55%

Detection of human cytomegalovirus in motile spermatozoa and spermatogenic cells in testis organotypic culture

et al. 2011

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BackgroundThe presence of human cytomegalovirus (HCMV) in male genital tract suggests its vertical transmission with spermatozoa and the development of a potentially dangerous fetal infection. The objective of the present study was to evaluate the possibility of intracellular HCMV localization in male germ cells and to examine the effect of the virus on human spermatogenesis.MethodsSemen samples from 91 infertile and 47 fertile men were analyzed. HCMV was detected by real time PCR, rapid culture method and PCR in situ. Human testis organotypic culture and quantitative karyological analysis were used to investigate viral effects on spermatogenesis. Localization of HCMV in immature germ cells and spermatozoa was studied by immunostaining with monoclonal antibodies and ultrastructural analysis of infected organotypic culture.ResultsViral DNA was detected in 12.3% samples of motile spermatozoa, while infectious activity only in 2.9% infertile and fertile men without statistically significant intergroup difference. According to PCR in situ, the mean percentage of infected cell in both groups was 1.5% (0.25%-15%), which can serve as a criterion for evaluating the risk of HCMV transmission. In HCMV-infected organotypic culture viral antigens were identified in spermatides on day 4, in spermatogonia and spermatocytes on day 8, and in spermatozoa on day 14. Empty and full capsides and virions were visualized in germ cells by electron microscopy. The number of cells before introduction in culture was taken for 100%. On day 14 infected culture contained 36.8% spermatogonia, 18.7% spermatocytes, 27.6% round spermatides and 42.5% elongated spermatides; in comparison with 82.2%, 51.5%, 70.4% and 65.7% in uninfected culture, respectively (all p < 0.05). There were no changes in the number and viability of spermatozoa.ConclusionsHCMV was detected in male germ cells, both in sperm samples and in testis organotypic culture. The virus may infect immature germ cells which develop to mature HCMV-carrying spermatozoa. A considerable decrease in the number of immature germ cells indicates that HCMV produces a direct gametotoxic effect and can contribute to male infertility.

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Section: Discussionsupporting

confidence: 55%

Detection of human cytomegalovirus in motile spermatozoa and spermatogenic cells in testis organotypic culture

et al. 2011

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“…_ENREF_16 It has been reported that HSV-2 and HCMV can associate with the sperm membrane [13], whereas others have found that HSV-2 can be internalized into heads of morphological normal and motile sperm [14], [15]. HCMV may enter the germ cells at an early time point.…”

Section: Introductionmentioning

confidence: 99%

Human Herpesvirus-6A/B Binds to Spermatozoa Acrosome and Is the Most Prevalent Herpesvirus in Semen from Sperm Donors

Kaspersen

Larsen²,

Kofod-Olsen

et al. 2012

PLoS ONE

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An analysis of all known human herpesviruses has not previously been reported on sperm from normal donors. Using an array-based detection method, we determined the cross-sectional frequency of human herpesviruses in semen from 198 Danish sperm donors. Fifty-five of the donors had at least one ejaculate that was positive for one or more human herpesvirus. Of these 27.3% (n = 15) had a double herpesvirus infection. If corrected for the presence of multiple ejaculates from some donors, the adjusted frequency of herpesviruses in semen was 27.2% with HSV-1 in 0.4%; HSV-2 in 0.1%; EBV in 6.3%; HCMV in 2.7%; HHV-6A/B in 13.5%; HHV-7 in 4.2%, whereas none of the samples had detectable VZV or HHV-8. Subsequently, we examined longitudinally data on ejaculates from 11 herpesvirus-positive donors. Serial analyses revealed that a donor who tested positive for herpesvirus at one time point did not necessarily remain positive over time. For the most frequently found herpesvirus, HHV-6A/B, we examined its association with sperm. For HHV-6A/B PCR-positive semen samples, HHV-6A/B could be detected on the sperm by flow cytometry. Conversely, PCR-negative semen samples were negative by flow cytometry. HHV-6B was shown to associate with sperm within minutes in a concentration dependent manner. Confocal microscopy demonstrated that HHV-6B associated with the sperm head, but only to sperm with an intact acrosome. Taken together, our data suggest that HHV-6A/B could be transported to the uterus via binding to the sperm acrosome. Moreover, we find a 10 times higher frequency of HHV-7 in semen from healthy individuals than previously detected. Further research is required to determine the potential risk of using herpesvirus-positive donor semen. Longitudinally analyses of ejaculate series indicate that implementation of quarantine for a donor shown to shed a herpesvirus is not a tenable solution.

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“…Later HSV DNA was detected by PCR in solitary ejaculate specimens collected between relapses of genital herpes (GH) [14]. Herpes simplex virus was detected in 24-30% ejaculate specimens from patients with a history of GH and fertility disorders [1,2,6]. The low level of PCRdetection of HSV in the ejaculate of patients without symptoms of GH can be explained by specific features of the semen as the substrate for such a study [11].…”

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confidence: 99%

Detection of type 2 herpes simplex virus in cells of spermatogenic epithelium in infected testes of guinea pigs

et al. 2007

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Cited by 8 publications

References 9 publications

Detection of human cytomegalovirus in motile spermatozoa and spermatogenic cells in testis organotypic culture

Detection of human cytomegalovirus in motile spermatozoa and spermatogenic cells in testis organotypic culture

Human Herpesvirus-6A/B Binds to Spermatozoa Acrosome and Is the Most Prevalent Herpesvirus in Semen from Sperm Donors

Detection of type 2 herpes simplex virus in cells of spermatogenic epithelium in infected testes of guinea pigs

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