Detection of type 2 herpes simplex virus in cells of spermatogenic epithelium in infected testes of guinea pigs

Gribencha, S. V.; Брагина, Е. Е.; Abdumalikov, R. A.; Bocharova, E. N.; Kurilo, L. F.

doi:10.1007/s10517-007-0258-9

Cited by 5 publications

(3 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…HSV-2 bound to human ejaculated sperm in vitro and slightly decreased their motility, but HSV-2 binding was impaired by seminal plasma (529). HSV-1 and -2 DNA were reported in spermatogenetic cells from men and guinea pigs using in situ hybridization (256,370). Since all of these studies only investigated infertile men or men seeking fertility evaluation, it would be important to compare the prevalence of HSV-1 and HSV-2 in semen from fertile versus infertile men in large cohorts with similar age, race, geographical and socio-economic criteria (case-control study) to confront the conflicting data on the potential involvement of HSV in male infertility.…”

Section: Human Immunodeficiency Virusmentioning

confidence: 99%

From Ancient to Emerging Infections: The Odyssey of Viruses in the Male Genital Tract

Tortorec

Matusali

Mahé

et al. 2020

Physiological Reviews

103

View full text Add to dashboard Cite

The male genital tract (MGT) is the target of a number of viral infections that can have deleterious consequences at the individual, offspring, and population levels. These consequences include infertility, cancers of male organs, transmission to the embryo/fetal development abnormalities, and sexual dissemination of major viral pathogens such as human immunodeficiency virus (HIV) and hepatitis B virus. Lately, two emerging viruses, Zika and Ebola, have additionally revealed that the human MGT can constitute a reservoir for viruses cleared from peripheral circulation by the immune system, leading to their sexual transmission by cured men. This represents a concern for future epidemics and further underlines the need for a better understanding of the interplay between viruses and the MGT. We review here how viruses, from ancient viruses that integrated the germline during evolution through old viruses (e.g., papillomaviruses originating from Neanderthals) and more modern sexually transmitted infections (e.g., simian zoonotic HIV) to emerging viruses (e.g., Ebola and Zika) take advantage of genital tract colonization for horizontal dissemination, viral persistence, vertical transmission, and endogenization. The MGT immune responses to viruses and the impact of these infections are discussed. We summarize the latest data regarding the sources of viruses in semen and the complex role of this body fluid in sexual transmission. Finally, we introduce key animal findings that are relevant for our understanding of viral infection and persistence in the human MGT and suggest future research directions.

show abstract

Section: Human Immunodeficiency Virusmentioning

confidence: 99%

From Ancient to Emerging Infections: The Odyssey of Viruses in the Male Genital Tract

Tortorec

Matusali

Mahé

et al. 2020

Physiological Reviews

103

View full text Add to dashboard Cite

show abstract

“…We assumed that sperm infection occurs in the stage of immature germ cells. In experimental animals, we demonstrated the immature sperm cells are susceptible to herpes simplex virus [35] (Figure 5). The sperm from male partner of group with high level of spontaneous abortion or failure of in vitro fertilization were infected with HSV capsides more frequently than the control groups-68% versus 9% (p=0.003) [36].…”

Section: International Journal Of Cell Science and Molecular Biology Particles May Be Disordered Transportation Of Viral Proteinsmentioning

confidence: 92%

Spermatozoon as a Vehicle for Viruses

Брагина¹

2017

IJCSMB

View full text Add to dashboard Cite

In the last decade, a viral infection of semen and sperm is the subject of intensive research. The research of viral infection of spermatozoa and its consequences for reproduction reached its peak with the beginning and development of methods of artificial reproduction. There are number of viruses known that they can infect the semen-the human immunodeficiency virus (HIV), human papilloma virus (HPV), hepatitis B virus (HBV), hepatitis C virus (HCV), and herpes simplex virus (HSV). Intra-gamete viral infection of spermatozoa is a widespread phenomenon. It is not yet enough data that would determine the true role of vertical virus transmission for the infection spread, and for abnormalities at embryonic development. However, there are more data available that can prove these phenomena. Solution to the problem of the possibility of a viral infection in human sperm is of fundamental importance. If the germ cells are infected themselves, then the mechanisms of reproduction may be involved. In this case, specific antiviral therapy for men can prevent some cases of miscarriage at a natural pregnancy and improve the outcomes of ART applications.

show abstract

“…Excess cell death, on the other hand, can lead to defective spermatogenesis and even infertility (Celik‐Ozenci et al, ). Factors linked to high rates of germ cell apoptosis include pathogenic microorganisms (Guazzone et al, ; Noguchi et al, ), electromagnetic radiation, environmental toxicants, heavy metals, and chemotherapeutic agents (Shaha et al, )—all of which can result in azoospermia and infertility (Gribencha et al, ; Schautteet and Vanrompay, ; Rivera‐Benitez et al, ).…”

Section: Introductionmentioning

confidence: 99%

Lipopolysaccharide‐induced expression of FAS ligand in cultured immature boar sertoli cells through the regulation of pro‐inflammatory cytokines and miR‐187

Wang

Zhang

Yang

et al. 2015

Molecular Reproduction Devel

View full text Add to dashboard Cite

Lipopolysaccharide (LPS) induces germ cell apoptosis, but its mechanism of action is not clear. One possibility is that LPS regulates the expression of FAS ligand (FASLG) in Sertoli cells, which will then influence germ cell apoptosis. In this study, LPS reduced the viability of cultured, immature boar Sertoli cells in a time- and dose-dependent manner; enhanced the production of pro-inflammatory cytokines including tumor necrosis factor α (TNFA), interleukin-1β (IL1B), nitric oxide (NO), and transforming growth factor-β (TGFB); and increased the expression of FASLG in a dose-dependent manner. While 10 μg/ml LPS enhanced the expression of FASLG, reduced cell cycle progression, and impaired the ultrastructure of Sertoli cells, this dose did not induce apoptosis. LPS also had no effect on the activity or expression of matrix metalloproteinases 2 or 9 (MMP2 or MMP9). In contrast, the expression of ssc-miR-187 increased following LPS challenge, and inhibition of ssc-miR-187 blocked LPS-induced expression of FASLG. Our results therefore suggest that LPS reduces the viability of and enhances FASLG expression in cultured, immature boar Sertoli cells through elevated secretion of TNFA, IL1B, NO, and TGFB as well as through the regulation of ssc-miR-187 potency.

show abstract

Detection of type 2 herpes simplex virus in cells of spermatogenic epithelium in infected testes of guinea pigs

Abstract: We developed a model of herpetic orchitis in guinea pigs. Intratesticular inoculation of type 2 herpes simplex virus suspension results in infection of the testicular spermatocytes and spermatides. The possibility of viral infection dissemination from infected into intact testis is proven.

Cited by 5 publications

References 10 publications

From Ancient to Emerging Infections: The Odyssey of Viruses in the Male Genital Tract

From Ancient to Emerging Infections: The Odyssey of Viruses in the Male Genital Tract

Spermatozoon as a Vehicle for Viruses

Lipopolysaccharide‐induced expression of FAS ligand in cultured immature boar sertoli cells through the regulation of pro‐inflammatory cytokines and miR‐187

Contact Info

Product

Resources

About