2006
DOI: 10.1074/jbc.m512379200
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9-O-Acetylation of Exogenously Added Ganglioside GD3

Abstract: Sialic acids are sometimes 9-O-acetylated in a developmentally regulated and cell-type-specific manner. Cells naturally expressing the disialoganglioside GD3 often O-acetylate the terminal sialic acid residue, giving 9-O-acetyl-GD3 (9AcGD3), a marker of neural differentiation and malignant transformation. We also reported that Chinese hamster ovary cells transfected with GD3 synthase can spontaneously O-acetylate some of the newly synthesized GD3. It is unclear whether such phenomena result from induction of t… Show more

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Cited by 27 publications
(20 citation statements)
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“…The acetylation of GD3, the fraction heavily accumulated in GM2/GD2 synthase-deficient mice, was previously confirmed by immunohistochemical localization and mass spectrometry in studies by Matsuda et al 23 and Furukawa et al 24 We did not detect O-Ac-GD3 in wt mouse brain tissue since it is a very minor ganglioside in the adult brain, while in developing brain, the expression of O-Ac-GD3 has been associated to processes such as neurite outgrowth and neuronal migration in mammalian brain. [31][32][33] Interestingly, it was found that exogenously added GD3 induces its own O-acetylation machinery in different cell types, 34 while other gangliosides fail to induce the same machinery. This finding implies that O-acetylation machinery requires very specific oligosaccharide moieties of gangliosides and that not all ganglioside species are susceptible to O-acetylation in the same proportion.…”
Section: Resultsmentioning
confidence: 99%
“…The acetylation of GD3, the fraction heavily accumulated in GM2/GD2 synthase-deficient mice, was previously confirmed by immunohistochemical localization and mass spectrometry in studies by Matsuda et al 23 and Furukawa et al 24 We did not detect O-Ac-GD3 in wt mouse brain tissue since it is a very minor ganglioside in the adult brain, while in developing brain, the expression of O-Ac-GD3 has been associated to processes such as neurite outgrowth and neuronal migration in mammalian brain. [31][32][33] Interestingly, it was found that exogenously added GD3 induces its own O-acetylation machinery in different cell types, 34 while other gangliosides fail to induce the same machinery. This finding implies that O-acetylation machinery requires very specific oligosaccharide moieties of gangliosides and that not all ganglioside species are susceptible to O-acetylation in the same proportion.…”
Section: Resultsmentioning
confidence: 99%
“…4) that could not be accurately quantified by TLC analysis; the large number of cells required for ganglioside extraction exceeded the capacity of reproducible transfection protocols, and the subcellular distribution of the gangliosides could not be determined by TLC. Therefore, we used flow cytometry, a method capable of detecting small shifts in cellular distributions of gangliosides such as GD3 (28,35,(47)(48)(49)(50) in this study. However, to address concerns raised by reports that work-up conditions alter cellular and tissue distributions of gangliosides (51) and to ensure that the flow cytometry method provided biologically-relevant results in our hands, we tested the concentration and time dependence of the uptake of endogenous GM3 and GD3.…”
Section: Verification Of the Flow Cytometry Detection Methods By Analymentioning
confidence: 99%
“…A second purpose for the characterization of ganglioside uptake by flow cytometry was to verify the mechanism of cellular uptake and incorporation of exogenous GM3 and GD3 and their subsequent ability to function as endogenous gangliosides (35,41). These experiments could provide evidence, albeit indi- (from B).…”
Section: Verification Of the Flow Cytometry Detection Methods By Analymentioning
confidence: 99%
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