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Abstract

Lactose-carrying styrene (VLA)-grafted polystyrene (PS) dish (PS-VLA) was prepared by treatment of PS dish with oxygen plasma glow discharge followed by the graft polymerization of VLA. The surface topology and hepatocytes behavior on PS-VLA were examined by comparison with those on a PVLA-coated PS dish (PS-PVLA). According to the results of surface topologies obtained by a phase mode of atomic force microscope (AFM), it was found that PS-VLA exhibits a pointed texture image similar to forest while PS-PVLA exhibits a phase-separated, cloud-like image. In an experiment involving hepatocytes adhesion, the cells more slowly adhered to PS-VLA than to PS-PVLA during the first 2 h incubation. According to topological data, it may be suggested that lactose density on the air side surface of PS-VLA is lower than that of PS-PVLA, thus leading to the slow adhesion of hepatocytes to PS-VLA.

A thermoresponsive and chemically defined hydrogel for long-term culture of human embryonic stem cells

“…To our knowledge, our study is the first to report long-term culture and gentle serial passaging of hESCs using a thermomodulatable synthetic polymer, verified with independent cell lines. Plasma treatment of plastic surfaces37 followed by simple addition of the appropriate monomers permitted HG polymer coating of conventional plasticware. This surface substrate should therefore be readily applicable in the future to scaled production of hESC in automated systems for industrial and clinical purposes.…”