Co-culture of hepatocytes and fibroblasts by micropatterned immobilization of β-galactose derivatives

Engineering the Extracellular Environment: Strategies for Building 2D and 3D Cellular Structures

“…[ 173 ] Such surface coating provides also selectivity because co-culture studies with hepatocytes and fi broblasts show preferential adhesion and growth of hepatocytes on galactose-modifi ed surface areas, while attachment of fi broblasts to the hydrophilic glycan-coating was inhibited. [ 174 ] Further studies were conducted to explore the potential of other glycans to guide adhesion of cells, such as mannose and lactose that promoted adhesion and growth of chondrocytes, while adhesion and growth of chondrosarcoma cells were inhibited. [ 175 ] The effect of different covalently bound di-and trisaccharides was studied, fi nding that cellulobiose and cellulotriose promoted adhesion of fi broblast while other sugars were inhibitory.…”

Synthesis of photoreactive pullulan for surface modification

“…Barbucci et al [18] synthesized photoreactive hyaluronan and its sulfated derivative for micropattern immobilization. We have modified growth factors [19], sulfated hyaluronic acid [20], heparin [21], thermo-responsive polymers [22], and b-galactose derivatives [23], and photoimmobilized them on various surfaces using the method devised by Matsuda and Sugawara [24]. This photoimmobilization method can be applied to various materials that have organic surfaces.…”

Scanning Probe Nanolithography and Protein Patterning of Low‐Fouling Plasma Polymer Multilayer Films

“…[1] Commonly, proteins are arrayed and patterned at micrometer-scale resolution. [2][3][4] However, the further miniaturization of patterning techniques, and hence increased biomolecule surface density, aims to push sensing resolution to the single-molecule limit. [5][6][7] Here, we demonstrate a novel technique to produce nanometer-scale spatial dimension protein patterns with a resolution of ca.…”