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Ilina, Elena; Malakhova, Maja V.; Vereshchagin, V. A.; Govorun, Vadim M.; Сергиенко, В. И.; Зубков, М. М.; Vasil'ev, M. M.; Кубанова, А. А.

doi:10.1023/a:1026379209810

Cited by 5 publications

(6 citation statements)

References 9 publications

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“…By using the duplex PCR, we found 31.1% of the 315 isolates tested were positive for the porB1A gene and the porB1B gene was detectable in 68.9% of the isolates. This porB1A + and porB1B + proportion in N. gonorrhoeae isolates is close to that of previous reports [21,22]. …”

Section: Discussionsupporting

confidence: 91%

“…PorB1A and PorB1B are encoded by the same allele and any particular N. gonorrhoeae strain expresses either PorB1A or PorB1B [12,19,20]. PorB1A is present in 10-30% of N. gonorrhoeae isolates, while PorB1B occurs in 70-90% [21,22]. PorB1A and PorB1B are the serotyping basis of N. gonorrhoeae and mutations are more common in porB1B gene than in porB1A gene [19,22,23].…”

Section: Introductionmentioning

confidence: 99%

“…PorB1A is present in 10-30% of N. gonorrhoeae isolates, while PorB1B occurs in 70-90% [21,22]. PorB1A and PorB1B are the serotyping basis of N. gonorrhoeae and mutations are more common in porB1B gene than in porB1A gene [19,22,23]. Many investigations revealed geographical diversity of the predominant porB1A and porB1B genotypes isolates in different areas [22,24-27].…”

Section: Introductionmentioning

confidence: 99%

“…PorB1A and PorB1B are the serotyping basis of N. gonorrhoeae and mutations are more common in porB1B gene than in porB1A gene [19,22,23]. Many investigations revealed geographical diversity of the predominant porB1A and porB1B genotypes isolates in different areas [22,24-27]. Therefore, determination of porB1A and porB1B genotype distribution in N. gonorrhoeae isolates in different areas is very important for providing a high index of discrimination of different gonococcal strains, identifying the circulating strains and predominant serotypes, and tracking strain transmission in sexual contacts [28-32].…”

Section: Introductionmentioning

confidence: 99%

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Predominant porB1A and porB1B genotypes and correlation of gene mutations with drug resistance in Neisseria gonorrhoeae isolates in Eastern China

Sun

Fan

et al. 2010

BMC Infect Dis

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BackgroundVariations of porB1A and porB1B genes and their serotypes exist in Neisseria gonorrhoeae isolates from different geographical areas, and some site mutations in the porB1B gene correlate with drug resistance.MethodsThe β-lactamase production of N. gonorrhoeae isolates was determined by paper acidometric test and nitrocefin discs. The porB1A and porB1B genes of 315 non-penicillinase-producting N. gonorrhoeae (non-PPNG) strains were amplified by PCR for sequencing to determine serotypes and site mutations. A duplex PCR was designed to simultaneously detect both porB1A and porB1B genes. Penicillin and tetracycline resistance was assessed by an in vitro drug sensitivity test.ResultsOf the N. gonorrhoeae isolates, 31.1% tested positive for porB1A and 68.9% for porB1B genes. All the 98 porB1A+ isolates belonging to IA6 serotype with either no mutation at the 120 and 121 sites (88.8%) or a D120G (11.2%) mutation and were no resistance to both penicillin and tetracycline. Among the 217 porB1B+ isolates, 26.7%, 22.6% and 11.5% belonged to IB3, IB3/6 and IB4 serotypes, respectively. Particularly, two novel chimeric serotypes, IB3/6-IB2 and IB2-IB4-IB2, were found in 77 and 8 porB1B+ isolates. Two hundred and twelve (97.7%) of the porB1B+ isolates were presented G120 and/or A121 mutations with 163 (76.9%) at both sites. Interestingly, within the 77 porB1B+ isolates belonging to IB3/6-IB2 serotype, 15 were discovered to possess novel deletions at both A121 and N122 sites. All the replacement mutations at these sites in PorB1B were correlated with resistance and the deletion mutation showed the highest resistance.ConclusionN. gonorrhoeae isolates circulating in Eastern China include a sole PorB1A serotype (IA6) and five PorB1B serotypes. Multiple mutations in porB1B genes, including novel A121 and N122 deletions, are correlated with high levels of penicillin and tetracycline resistance.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Predominant porB1A and porB1B genotypes and correlation of gene mutations with drug resistance in Neisseria gonorrhoeae isolates in Eastern China

Sun

Fan

et al. 2010

BMC Infect Dis

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“…Nucleotide sequences of por gene in the studied strains were determined previously [1]. Amino acid sequences of Por protein were derived from the known nucleotide sequences using Vector NTIT R Suite v.6.0 software (Informax Inc.).…”

Section: Methodsmentioning

confidence: 99%

Analysis of genetic markers of N. Gonorrhoeae resistance to β-lactam antibiotics

et al. 2006

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A complex method for detection of genetic markers of N. gonorrhoeae resistance to penicillin was developed. Mutations in penA and ponA genes were detected by minisequencing reaction with subsequent detection of reaction products by MALDI-TOF mass spectrometry. This approach was tested on 31 clinical strains of N. gonorrhoeae with minimum inhibitory concentration of penicillin from 0.03 to 8 microg/ml and higher. Mutations in penA and ponA genes in moderately resistant strains were shown (minimum inhibitory concentration up to 0.5 microg/ml) and mutations in penA, ponA, and penB genes in resistant strains (minimum inhibitory concentration more than 1.0 microg/ml). beta-Lactamase genes were detected in 4 strains with high resistance (minimum inhibitory concentration 4-8 and more microg/ml). Correlation between microbiological resistance and presence of respective mutations in the studied locuses was detected.

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Clusters of circulating Neisseria gonorrhoeae strains and association with antimicrobial resistance in Shanghai

Liao¹,

Bell²,

Gu³

et al. 2008

Journal of Antimicrobial Chemotherapy

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Cited by 5 publications

References 9 publications

Predominant porB1A and porB1B genotypes and correlation of gene mutations with drug resistance in Neisseria gonorrhoeae isolates in Eastern China

Predominant porB1A and porB1B genotypes and correlation of gene mutations with drug resistance in Neisseria gonorrhoeae isolates in Eastern China

Analysis of genetic markers of N. Gonorrhoeae resistance to β-lactam antibiotics

Clusters of circulating Neisseria gonorrhoeae strains and association with antimicrobial resistance in Shanghai

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