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Insertion of an unnatural amino acid into the protein structure: preparation and properties of 3-fluorotyrosine-containing organophosphate hydrolase

“…The plasmid pTES His OPH encoding the synthesis of His 6 OPH was used for their transformation. 15 The E. coli B 2935 cells containing the plasmid pTES His OPH were incubated at 37 °C in the M9 medium containing (per L) glucose (4 g), thiamine (1 mmol), MgSO 4 (1 mmol), CaCl 2 (0.1 mmol), L tyrosine (25 µmol) and ampicil lin (100 mg).…”

Microbial Biosensors for Organophosphate Pesticides

“…OPAA has high specificity for hydrolysis of P-F and a P-CN bond present in nerve gases sarin and tabun [13] but is not capable of hydrolyzing OPs with P-O and P-S bonds present in widely used pesticides and insecticides, such as parathion, malathion, and demeton-S [14]. On the other hand, OPH catalyzes hydrolysis of the P-O, P-F, P-S, or P-CN bonds of various OP compounds, although to different extent as shown in Table 2, making it attractive for much broader spectrum of OPs [15]. OPH has been studied extensively over the last decade, and several genetically engineered variants have been produced in an effort to improve its catalytic ability [16,17].…”

Biomimetic electrochemical sensor based on molecularly imprinted polymer for dicloran pesticide determination in biological and environmental samples

“…Other determination methods reported by the literature are spectrophotometry [7,8], immunoassay [7], infrared spectroscopy [9], solid-phase extraction [10] or enzymatic techniques [11] sometimes in combination with mass spectrometry [12], or the flow injection technique [7,13]. There are some reasons to develop novel, simple, and inexpensive methods for determining the pesticides.…”