Microbial Biosensors for Organophosphate Pesticides

Mulchandani, Ashok; Rajesh, Rajesh

doi:10.1007/s12010-011-9288-x

Cited by 37 publications

(15 citation statements)

References 47 publications

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“…Organophosphorus compounds (OP) are neurotoxic chemicals that can be used as nerve agents (e.g., Sarin) or agricultural pesticides (e.g., parathion). , OP compounds present high toxicity to human health by inactivating acetylcholinesterase that is essential for proper functioning of nerve cells; therefore, it becomes important for us to detect even trace amounts of OP present as a chemical warfare agent or as an environmental contaminant. − …”

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confidence: 99%

“…Trace amount of OP can be detected using traditional analytical methods such as gas and liquid chromatography, which are nonportable and therefore unsuitable for on-site detection. As such, biosensors that offer portability as well as good specificity and sensitivity become attractive alternative methods for OP detection. ,, Among the various kinds of biosensors available, whole-cell biosensors are favorable due to their relatively low cost of sustainable production and operational stability. Whole-cell biosensors are usually designed to consist of sensing and reporter modules.…”

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Development of Colorimetric-Based Whole-Cell Biosensor for Organophosphorus Compounds by Engineering Transcription Regulator DmpR

Chong

Ching

2016

ACS Synth. Biol.

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It is useful for whole-cell biosensors to be based on colorimetric detection because the output signal can be easily visualized. However, colorimetric-based whole-cell biosensors suffer higher detection limits as compared to bioluminescence- or fluorescence-based biosensors. In this work, we attempt to reduce the detection limit for a colorimetric-based whole-cell biosensor by applying directed evolution techniques on a transcription regulator, DmpR, to alter the expression level of its cognate promoter, which was fused to mRFP1 to output red coloration in the presence of organophosphate pesticides containing a phenolic group. We selected the two best-performing mutants, DM01 and DM12, which were able to develop red coloration in the presence of parathion as low as 10 μM after just 6 h of induction at 30 °C. This suggests that engineering of the transcription regulator in the sensing domain is useful for improving various properties of whole-cell biosensors, such as reducing the detection limit for simple colorimetric detection of organophosphate pesticides.

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Development of Colorimetric-Based Whole-Cell Biosensor for Organophosphorus Compounds by Engineering Transcription Regulator DmpR

Chong

Ching

2016

ACS Synth. Biol.

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“…In this study, we have shown that engineered microbial combinations can both degrade and detect toxic OPs with incomparable specificity and sensitivity using electrochemical readout. 1,33,40,[45][46][47][48][49][50][51][52][53]65 E. coli were engineered to express OPH on the cell surface to degrade organophosphate pesticides to p-NP, even following E. coli lyophilization. We also engineered electroactive microbes, S. oneidensis, to express a critical EET protein, CymA, in the presence of p-NP.…”

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confidence: 99%

A Microbial Electrochemical Technology to Detect and Degrade Organophosphate Pesticides

et al. 2021

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Organophosphate (OP) pesticides cause hundreds of illnesses and deaths annually. Unfortunately, exposures are often detected by monitoring degradation products in blood and urine, with few effective methods for detection and remediation at the point of dispersal. We have developed an innovative strategy to remediate these compounds: an engineered microbial technology for the targeted detection and destruction of OP pesticides. This system is based upon microbial electrochemistry using two engineered strains. The strains are combined such that the first microbe ( E. coli ) degrades the pesticide, while the second ( S. oneidensis ) generates current in response to the degradation product without requiring external electrochemical stimulus or labels. This cellular technology is unique in that the E. coli serves only as an inert scaffold for enzymes to degrade OPs, circumventing a fundamental requirement of coculture design: maintaining the viability of two microbial strains simultaneously. With this platform, we can detect OP degradation products at submicromolar levels, outperforming reported colorimetric and fluorescence sensors. Importantly, this approach affords a modular, adaptable strategy that can be expanded to additional environmental contaminants.

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“…Pesticides block the catalytic activity of the active center, thus acting as inhibitors of AChE. This results in the accumulation of acetylcholine in the synaptic membrane, which blocks the nerves to process the signals properly [16].…”

Section: Principle Of Btamentioning

confidence: 99%

Acetylcholinesterase Based Detection of Residual Pesticides on Cotton

Hassan¹,

Militký²

2012

AJAC

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This study describes the measurement of bio-electrical signals caused by enzymatic inhibition of acetylcholinesterase (AChE) for the detection of organophosphorous and carbamate pesticides which are the strong inhibitors of AChE and prevents its normal function of the rapid removal of acetylcholine (Ach). Biosensor Toxicity Analyzer (BTA) was used for the testing and enzyme activity was determined by acetylthiocholine chloride (ATCCl) as enzyme substrate. The monitoring of changes in bio-electrical signals caused by the interaction of biological substances and residues were evaluated. Two samples of cotton were analyzed. Cryogenic homogenization was carried out for sample pretreatment and Soxhlet extraction method (SOX) was used for extraction. The resulted extracts were concentrated and then injected in the BTA. The method shows reasonable results and can successfully be utilized for the detection of residual pesticides on different types of cotton.

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Microbial Biosensors for Organophosphate Pesticides

Cited by 37 publications

References 47 publications

Development of Colorimetric-Based Whole-Cell Biosensor for Organophosphorus Compounds by Engineering Transcription Regulator DmpR

Development of Colorimetric-Based Whole-Cell Biosensor for Organophosphorus Compounds by Engineering Transcription Regulator DmpR

A Microbial Electrochemical Technology to Detect and Degrade Organophosphate Pesticides

Acetylcholinesterase Based Detection of Residual Pesticides on Cotton

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