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Vleeshouwers, Vivianne G. A. A.; Dooijeweert, W. van; Keizer, L.C.P.; Sijpkes, L.; Govers, F.; Colon, L.T.

doi:10.1023/a:1008710700363

Cited by 152 publications

(34 citation statements)

References 22 publications

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“…Detached leaves of potato (“E-potato-3”) of 8-week-old plants were inoculated with 10 µL zoospores from P. infestans isolate HB0914-2 at 5 × 10 4 mL −1 using the method of Vleeshouwers et al [38]. Leaf discs (1 cm diameter) of equal sizes surrounding the inoculation sites were collected at 0, 24, 48, and 72 h after inoculation and frozen in liquid nitrogen for immediate use or stored at −80 °C for RNA extraction.…”

Section: Methodsmentioning

confidence: 99%

The Cell Death Triggered by the Nuclear Localized RxLR Effector PITG_22798 from Phytophthora infestans Is Suppressed by the Effector AVR3b

Wang

Ren

Zhou

et al. 2017

IJMS

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Phytopathogenic oomycetes, such as Phytophthora infestans, potentially secrete many RxLR effector proteins into plant cells to modulate plant immune responses and promote colonization. However, the molecular mechanisms by which these RxLR effectors suppress plant immune responses are largely unknown. Here we describe an RxLR effector PITG_22798 (Gene accession: XM_002998349) that was upregulated during early infection of potato by P. infestans. By employment of agroinfiltration, we observed that PITG_22798 triggers cell death in Nicotiana benthamiana. Confocal microscopic examination showed that PITG_22798-GFP (Green Fluorescent Protein) located in the host nucleus when expressed transiently in N. benthamiana leaves. A nuclear localization signal (NLS) domain of PITG_22798 is important for nuclear localization and cell death-inducing activity. Sequence alignment and transient expression showed that PITG_22798 from diverse P. infestans isolates are conserved, and transient expression of PITG_22798 enhances P. infestans colonization of N. benthamiana leaves, which suggests that PITG_22798 contributes to P. infestans infection. PITG_22798-triggered cell death is dependent on SGT1-mediated signaling and is suppressed by the P. infestans avirulence effector 3b (AVR3b). The present research provides a clue for further investigation of how P. infestans effector PITG_22798 associates with and modulates host immunity.

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Section: Methodsmentioning

confidence: 99%

The Cell Death Triggered by the Nuclear Localized RxLR Effector PITG_22798 from Phytophthora infestans Is Suppressed by the Effector AVR3b

Wang

Ren

Zhou

et al. 2017

IJMS

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“…Rpi gene transformants were characterized using a detached leaf assay (DLA), in which eight 10 μl droplets of a zoospore suspension (50,000 spores/ml) of a specific Pi isolate (Vleeshouwers et al 1999) were applied to the abaxial side of the leaf. Criteria for scoring (DLA): R, all of the inoculation spots on a detached leaf showed resistance; RQ, 6–7 out of eight spots on a leaf showed resistance; Q, 3–5 out of eight spots on a leaf showed resistance; SQ, 1–2 out of eight spots on a leaf showed resistance; S, all spots on a leaf showed susceptibility.…”

Section: Methodsmentioning

confidence: 99%

Functional stacking of three resistance genes against Phytophthora infestans in potato

et al. 2011

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Functional stacking of broad spectrum resistance (R) genes could potentially be an effective strategy for more durable disease resistance, for example, to potato late blight caused by Phytophthora infestans (Pi). For this reason, three broad spectrum potato R genes (Rpi), Rpi-sto1 (Solanum stoloniferum), Rpi-vnt1.1 (S. venturii) and Rpi-blb3 (S. bulbocastanum) were selected, combined into a single binary vector pBINPLUS and transformed into the susceptible cultivar Desiree. Among the 550 kanamycin resistant regenerants, 28 were further investigated by gene specific PCRs. All regenerants were positive for the nptII gene and 23 of them contained the three Rpi genes, referred to as triple Rpi gene transformants. Detached leaf assay and agro-infiltration of avirulence (Avr) genes showed that the 23 triple Rpi gene transformants were resistant to the selected isolates and showed HR with the three Avr effectors indicating functional stacking of all the three Rpi genes. It is concluded that Avr genes, corresponding to the R genes to be stacked, must be available in order to assay for functionality of each stack component. No indications were found for silencing or any other negative effects affecting the function of the inserted Rpi genes. The resistance spectrum of these 23 triple Rpi gene transformants was, as expected, a sum of the spectra from the three individual Rpi genes. This is the first example of a one-step approach for the simultaneous domestication of three natural R genes against a single disease by genetic transformation.Electronic supplementary materialThe online version of this article (doi:10.1007/s11248-011-9510-1) contains supplementary material, which is available to authorized users.

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“…IPO-C was used in detached leaf assays as described by Vleeshouwers et al (1999) but also to inoculate field trials. In 2009 and 2010, respectively, four and two in vitro plants per genotype from population 3020 were planted in the beginning of June.…”

Section: Methodsmentioning

confidence: 99%

Mapping of the S. demissum late blight resistance gene R8 to a new locus on chromosome IX

Arens

Kim

et al. 2011

Theor Appl Genet

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The use of resistant varieties is an important tool in the management of late blight, which threatens potato production worldwide. Clone MaR8 from the Mastenbroek differential set has strong resistance to Phytophthora infestans, the causal agent of late blight. The F1 progeny of a cross between the susceptible cultivar Concurrent and MaR8 were assessed for late blight resistance in field trials inoculated with an incompatible P. infestans isolate. A 1:1 segregation of resistance and susceptibility was observed, indicating that the resistance gene referred to as R8, is present in simplex in the tetraploid MaR8 clone. NBS profiling and successive marker sequence comparison to the potato and tomato genome draft sequences, suggested that the R8 gene is located on the long arm of chromosome IX and not on the short arm of chromosome XI as was suggested previously. Analysis of SSR, CAPS and SCAR markers confirmed that R8 was on the distal end of the long arm of chromosome IX. R gene cluster directed profiling markers CDPSw54 and CDPSw55 flanked the R8 gene at the distal end (1 cM). CDPTm21-1, CDPTm21-2 and CDPTm22 flanked the R8 gene on the proximal side (2 cM). An additional co-segregating marker (CDPHero3) was found, which will be useful for marker assisted breeding and map based cloning of R8.

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Cited by 152 publications

References 22 publications

The Cell Death Triggered by the Nuclear Localized RxLR Effector PITG_22798 from Phytophthora infestans Is Suppressed by the Effector AVR3b

The Cell Death Triggered by the Nuclear Localized RxLR Effector PITG_22798 from Phytophthora infestans Is Suppressed by the Effector AVR3b

Functional stacking of three resistance genes against Phytophthora infestans in potato

Mapping of the S. demissum late blight resistance gene R8 to a new locus on chromosome IX

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