1999
DOI: 10.1023/a:1008763111230
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Cited by 34 publications
(2 citation statements)
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“…Several PCR protocols and specific primers were designed for detection or identification of the species and for phylotyping ( Table 2) (SEAL et al, 1993;ELPHINSTONE et al, 1996;OPINA et al, 1997;FEGAN et al, 1998;BOUDAZIN et al, 1999;PASTRIK;MAISS, 2000;LUISETTI, 2000;WELLER et al, 2000). Classification into phylotypes is done by PCR Multiplex with the Nmult series primers (based on ITS region) and the classification into sequevar by partially sequencing gene egl (encoding the enzyme endoglucanase).…”
Section: Genetic Diversity Of Ralstonia Solanacearum In Brazilmentioning
confidence: 99%
“…Several PCR protocols and specific primers were designed for detection or identification of the species and for phylotyping ( Table 2) (SEAL et al, 1993;ELPHINSTONE et al, 1996;OPINA et al, 1997;FEGAN et al, 1998;BOUDAZIN et al, 1999;PASTRIK;MAISS, 2000;LUISETTI, 2000;WELLER et al, 2000). Classification into phylotypes is done by PCR Multiplex with the Nmult series primers (based on ITS region) and the classification into sequevar by partially sequencing gene egl (encoding the enzyme endoglucanase).…”
Section: Genetic Diversity Of Ralstonia Solanacearum In Brazilmentioning
confidence: 99%
“…A method utilising postreaction RFLP analysis to distinguish infraspecific R. solanacearum strains at the biovar level was also developed (Poussier and Luisetti 2000). Primer sets were also developed to enable the specific identification of divisions within the species by targeting the 16S rDNA gene (Fegan et al 1998;Boudazin et al 1999), and the intergenic spacer region between the 16S and 23S rDNA genes (Pastrik et al 2000). Multiplex TaqMan methods specific for the detection of all strains of R. solanacearum (Weller et al 2000) as well as race 3 biovar 2, (Weller et al 2000), permitted analysis in real-time PCR within a closed tube system, reducing the risk of cross-contamination within the laboratory.…”
mentioning
confidence: 99%