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Jara, Adelina de la; Mendoza, H.; Martel, A. Conde; Molina, Cristina; Nordströn, Laurette; Rosa, Vladimir de la; Diaz, Ricardo J.

doi:10.1023/a:1026007902078

Cited by 114 publications

(33 citation statements)

References 19 publications

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“…Approximately 75% of the harmful algal bloom species are dinoflagellates (Smayda 1997). As a result of their very high concentrations during explosive and extensive blooms, they have been studied as a potential candidate for a source of biofuel (de la Jara et al 2003, Fuentes-Grünewald et al 2012. Thus, it is very important to analyze the lipid content of many dinoflagellates within relatively short time by using reasonable lipid quantification methods.…”

Section: Color Reaction Of Four Dinoflagellate Species In Different Cmentioning

confidence: 99%

Fatty acid composition and docosahexaenoic acid (DHA) content of the heterotrophic dinoflagellate Oxyrrhis marina fed on dried yeast: compared with algal prey

Yoon¹,

Park²,

Jeong³

et al. 2017

ALGAE

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To develop an easy and rapid method of quantifying lipid contents of marine dinoflagellates, we quantified lipid contents of common dinoflagellate species using a colorimetric method based on the sulpho-phospho-vanillin reaction. In this method, the optical density measured using a spectrophotometer was significantly positively correlated with the known lipid content of a standard oil (Canola oil). When using this method, the lipid content of each of the dinoflagellates Alexandrium minutum, Prorocentrum micans, P. minimum, and Lingulodinium polyedrum was also significantly positively correlated with the optical density and equivalent intensity of color. Thus, when comparing the color intensity or the optical density of a sample of a microalgal species with known color intensities or optical density, the lipid content of the target species could be rapidly quantified. Furthermore, the results of the sensitivity tests showed that only 1-3 × 10 5 cells of P. minimum and A. minutum, 10 4 cells of P. micans, and 10 3 cells of L. polyedrum (approximately 1-5 mL of dense cultures) were needed to determine the lipid content per cell. When the lipid content per cell of 9 dinoflagellates, a diatom, and a chlorophyte was analyzed using this method, the lipid content per cell of these microalgae, with the exception of the diatom, were significantly positively correlated with cell size, however, volume specific lipid content per cell was negatively correlated with cell size. Thus, this sulpho-phospho-vanillin method is an easy and rapid method of quantifying the lipid content of autotrophic, mixotrophic, and heterotrophic dinoflagellate species.Key Words: cell size; intensity of color; lipid amount; lipid content per cell; sulfo-phospho-vanilin (SPV); volume specific lipid content INTRODUCTIONKnowing the lipid content of marine microalgae is important for understanding their metabolism and for the use of their biomass as biofuel. Among them, dinoflagellates contain large amounts of high-quality lipids and dinosterols (Piretti et al. 1997, Mansour et al. 1999.Dinoflagellates play diverse roles in the marine food web as primary producers, prey, predators, symbionts, and parasites (Muscatine 1990, Coats 1999, Jeong et al. 2010, Lee et al. 2014a, 2014b. In the last two decades, many phototrophic dinoflagellates that were previously thought to be exclusively autotrophic have been shown to be mixotrophic (Jacobson and Anderson 1996, Stoecker 1999, Jeong et al. 2005, Lee et al. 2014c. Therefore, these protists not only perform photosynthesis, but also feed on prey as energy sources. Furthermore, many dinoflagellates are important prey for mixotrophic Received October 3, 2016, Accepted December 7, 2016 *Corresponding Author E-mail: bada0@snu.ac.kr Tel: This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original wo...

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Section: Color Reaction Of Four Dinoflagellate Species In Different Cmentioning

confidence: 99%

Fatty acid composition and docosahexaenoic acid (DHA) content of the heterotrophic dinoflagellate Oxyrrhis marina fed on dried yeast: compared with algal prey

Yoon¹,

Park²,

Jeong³

et al. 2017

ALGAE

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“…The cell size and granularity increase when C. cohnii cells rest in the G 1 -phase of the cell cycle [66,67] and when they accumulate DHA in the stationary phase. In addition, de la Jara et al [89] demonstrated the linear correlation between Nile Red staining intensity and DHA content in C. cohnii cells. This methodology was applied in our lab with similar outcomes [89].…”

Section: Integration Of Flow Cytometry In Process Developmentmentioning

confidence: 97%

“…In addition, de la Jara et al [89] demonstrated the linear correlation between Nile Red staining intensity and DHA content in C. cohnii cells. This methodology was applied in our lab with similar outcomes [89].…”

Section: Integration Of Flow Cytometry In Process Developmentmentioning

confidence: 97%

Cultivation of Marine Microorganisms in Single-Use Systems

Hillig

Pilarek

Junne

et al. 2013

Advances in Biochemical Engineering/Biotechnology

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: Marine cultures are an important source of novel substances and enzymes. As efforts to isolate strains from (deep) sea environments increase, the demand for methodology platforms to cultivate these organisms is also rising. Due to the high salt concentration and the shear sensitivity exhibited by some heterotrophic microalgae, single-use systems originally designed for the cultivation of mammalian cell lines can be a valuable alternative. Using the cultivation of the heterotrophic marine microalgae Crypthecodinium cohnii as an example, this chapter makes suggestions for experimental design, for improving process development by integrating parallel experiments, and for scaling-up and scaling-down methodologies. It describes how to identify suitable single-use systems and how to integrate a two-layer system with perfluordecalin to improve the gas transfer in deep-well plates. The process is also scaled up in several single-use systems. We also describe challenges in the process development to achieve sufficient oxygen transfer, monitoring, and control, and we discuss limitations such as corrosion, long-term stability, and leachables in single-use systems. Finally, we demonstrate a method for cheap, fast, and consistent process development for marine microorganisms.

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“…Here, it is reported that a slight decrease in biomass productivity of the NH 4 Cl group was found and lipid productivity of S. obliquus was demonstrated to be higher than that of other tested nitrogen sources. The use of flow-cytometric analysis for determination of lipid accumulations in algae cells were successfully shown in previous studies [21]. Because gravimetric and microplate fluorometric analyses could not give information about the single cell level, flow-cytometric analysis was also conducted for determination of the response of single cells.…”

Section: Protein Chlorophyll and Carotenoid Analysesmentioning

confidence: 99%

Nitrogen Source, an Important Determinant of Fatty Acid Accumulation and Profile in Scenedesmus obliquus

2016

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The potential of algae-based fuel technologies for manufacturing renewable biofuels has been attracting interest from the scientific community. Biomass productivity and cellular lipid content are important parameters affecting the feasibility of using algae oil for biodiesel production. This study compares utilization of NaNO3, NH4Cl and urea as different nitrogen sources in terms of their effects on biomass productivity, fatty acid profile and accumulation in Scenedesmus obliquus. Cellular lipid accumulation was analyzed by gravimetric, fluorometric, and flow-cytometric methods, besides collecting spectrophotometric data for biomass productivity analysis. In addition, fatty acid profiles were compared by using gas chromatography-mass spectrometry. The alga can utilize all tested nitrogen sources successfully however growth rates demonstrate differences. Gravimetric lipid content analysis showed approximately a ≈1.5-fold increase in total lipid accumulation under NH4Cl regime when compared to that of NaNO3 and a ≈2-fold increase when compared to that of urea at the end of ten days cultivation course. Fatty acid profiles under different nitrogen regimes present variations especially under NH4Cl regime. Moreover, all lipid extracts mostly consist of saturated, straight-and branched-chain hydrocarbons of different chain lengths ranging from C16-C20 which grant a suitable profile for biodiesel production. Scenedesmus obliquus is a suitable species for biodiesel production. The results obtained from this study provide a better understanding of cultivation characteristics of this important species and support potential, future biodiesel production.

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Cited by 114 publications

References 19 publications

Fatty acid composition and docosahexaenoic acid (DHA) content of the heterotrophic dinoflagellate Oxyrrhis marina fed on dried yeast: compared with algal prey

Fatty acid composition and docosahexaenoic acid (DHA) content of the heterotrophic dinoflagellate Oxyrrhis marina fed on dried yeast: compared with algal prey

Cultivation of Marine Microorganisms in Single-Use Systems

Nitrogen Source, an Important Determinant of Fatty Acid Accumulation and Profile in Scenedesmus obliquus

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