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Lin, Han-wei; Kwok, Kian H.; Doran, Pauline M.

doi:10.1023/a:1022821600283

Cited by 45 publications

(15 citation statements)

References 13 publications

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“…In Linum flavum , A . rhizogenes strains LBA 9402 and TR 105 induced hairy roots with 50% frequency from leaf discs whereas low induction was observed with strains 15834 and A4 [24] . Variable degree of transformation efficiency was also observed in Beta vulgaris transformed with different Agrobacterium strains [25] and from root segments of Maytenus senegalensis using strains LBA 9402 and A4T [26] .…”

Section: Discussionmentioning

confidence: 92%

Hairy root induction and phytoremediation of textile dye, Reactive green 19A-HE4BD, in a halophyte, Sesuvium portulacastrum (L.) L.

Lokhande¹,

Kudale

Nikalje

et al. 2015

Biotechnology Reports

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Section: Discussionmentioning

confidence: 92%

Hairy root induction and phytoremediation of textile dye, Reactive green 19A-HE4BD, in a halophyte, Sesuvium portulacastrum (L.) L.

Lokhande¹,

Kudale

Nikalje

et al. 2015

Biotechnology Reports

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“…HR phenotype is characterized by vigorous growth, lack of geotropism, high lateral branching and genetic stability in hormone free medium [ 30 ]. L. flavum HR lines have been previously established and described as an effective system for the production of ATL including up to 3.5% DW of MPT [ 25 , 31 ] or lignan precursor such as coniferin [ 32 ]. However, in these studies few independent HR lines have been generated and analyzed while in the absence of a thorough assessment of the influence of growing conditions on ATL accumulation it is difficult to get a clear idea of the production potential of this species.…”

Section: Introductionmentioning

confidence: 99%

Investigation of Linum flavum (L.) Hairy Root Cultures for the Production of Anticancer Aryltetralin Lignans

Renouard

Corbin

Drouet

et al. 2018

IJMS

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Linum flavum hairy root lines were established from hypocotyl pieces using Agrobacterium rhizogenes strains LBA 9402 and ATCC 15834. Both strains were effective for transformation but induction of hairy root phenotype was more stable with strain ATCC 15834. Whereas similar accumulation patterns were observed in podophyllotoxin-related compounds (6-methoxy-podophyllotoxin, podophyllotoxin and deoxypodophyllotoxin), significant quantitative variations were noted between root lines. The influence of culture medium and various treatments (hormone, elicitation and precursor feeding) were evaluated. The highest accumulation was obtained in Gamborg B5 medium. Treatment with methyl jasmonate, and feeding using ferulic acid increased the accumulation of aryltetralin lignans. These results point to the use of hairy root culture lines of Linum flavum as potential sources for these valuable metabolites as an alternative, or as a complement to Podophyllum collected from wild stands.

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“…[16] In L. flavum , hairy roots were initiated from leaf discs with a success rate of approximately 50% using A. rhizogenes strains ‘LBA9402’and ‘TR105’. [6] Evidently, the selection of an effective bacterial strain for the production of transformed root cultures is highly dependent on the plant species, and must be determined empirically. [17] The bacterial strains used in this study exhibit different levels of virulence to this plant species and the induction of hairy root occurred at different time depended on strains.…”

Section: Resultsmentioning

confidence: 99%

“…such as L. flavum ,[6] L. austriacum ,[7] L. tauricum [8] and main metabolites (lignans) from resulted hairy roots have been shown to have biological activity.…”

Section: Introductionmentioning

confidence: 99%

Podophyllotoxin and 6-methoxy podophyllotoxin Production in Hairy Root Cultures of Liunm mucronatum ssp. mucronatum

et al. 2014

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Aim:Two bacterial strains of Agrobacterium rhizogenes, A13 and 9534 were evaluated for induction of transformed hairy roots in Linum mucronatum ssp. mucronatum, a high value medicinal plant.Materials and Methods:The hairy roots were successfully initiated, through infecting the hypocotyl and root explants and the A13 strain performed a high transformation frequency for hairy roots induction. Transgenic status of hairy roots was confirmed by polymerase chain reaction (PCR) analysis of the rol genes. Growth kinetics of transgenic roots induced by two strains indicated a similar pattern of growth, with maximum growth occurring between 42 to 56 days. The lignan contents in hairy roots were analyzed using high-performance liquid chromatography (HPLC) method.Results:Transformed cultures showed significant differences (P < 0.05) in lignan content. The highest amount of Podophyllotoxin (PTOX, 5.78 mg/g DW) and 6-methoxy podophyllotoxin (MPTOX, 49.19 mg/g DW) was found in transformed lines induced by strain A13, which was four times higher than those of non-transformed roots. The results showed that hairy root cultures of L. mucronatum are rich sources of MPTOX.Conclusion:hairy root cultures from L. mucronatum can be used as a useful system for scale-up producing MPTOX and precursors for the production of antitumor agents in substitution with PTOX by considering the appropriate optimizations in future studies.

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Untitled

Cited by 45 publications

References 13 publications

Hairy root induction and phytoremediation of textile dye, Reactive green 19A-HE4BD, in a halophyte, Sesuvium portulacastrum (L.) L.

Hairy root induction and phytoremediation of textile dye, Reactive green 19A-HE4BD, in a halophyte, Sesuvium portulacastrum (L.) L.

Investigation of Linum flavum (L.) Hairy Root Cultures for the Production of Anticancer Aryltetralin Lignans

Podophyllotoxin and 6-methoxy podophyllotoxin Production in Hairy Root Cultures of Liunm mucronatum ssp. mucronatum

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