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Sordillo, Lorraine M.; SooHoo, H.; Aherne, K. M.; Reddy, C. Channa; Hogan, J.S.

doi:10.1023/a:1009759132250

Cited by 21 publications

(2 citation statements)

References 13 publications

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“…Bovine aortic endothelial cells (BAEC) were collected from the aorta of healthy Holstein dairy cows based on techniques previously described by Sordillo et al (1998). The BAECs were purified by dilution cloning techniques and then cultured in Ham's F-12K media containing 10% FBS, 20 mM HEPES, 2 mM L-glutamine, 1% (vol/vol) antibiotic-antimycotic, heparin (100 µg/ml), insulin (10 µg/ml), transferrin (5 µg/ml), and sodium selenite (10 ng/ml).…”

Section: Bovine Aortic Endothelial Cell Isolation and Culturingmentioning

confidence: 99%

Pomegranate (Punica granatum) By-Product Extract Influences the Oxylipids Profile in Primary Bovine Aortic Endothelial Cells in a Model of Oxidative Stress

et al. 2022

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Aerobic metabolism produces reactive oxygen species (ROS) as a natural by-product that can play a significant role in cell signaling and homeostasis. Excessive and uncontrolled production of ROS, however, can lead to oxidative stress that causes damage to immune cells and is related to several diseases in dairy cattle. Endothelial cells are essential for optimal immune and inflammatory responses but are especially sensitive to the damaging effects of ROS. Accordingly, investigating antioxidant strategies that can mitigate the detrimental impact of ROS on endothelial functions could impact compromised host defenses that lead to increased disease susceptibility. The objective of this study was to test the antioxidant effect of different concentrations (20, 40, 60, 80 μg/ml) of pomegranate by-product extract (PBE) on bovine aortic endothelial cells (BAECs). A model of oxidative stress was developed using in vitro exposure of BAEC to 2,2′-azobis (2-amidinopropane) dihydrochloride (AAPH) to induce the formation of ROS. The BAEC were then analyzed for cell viability, ROS production, fatty acids profile, and oxylipids formation. The BAECs viability did not change after different concentrations of PBE and remained up to 80% over control; whereas, intracellular ROS showed a reduction passing from 20 to 50% with increasing PBE concentration from 20 to 80 μg/ml, respectively. The PBE extract clearly demonstrated efficacy in reducing the concentrations of pro-inflammatory oxylipids with a concomitant enhancement of anti-inflammatory oxylipids. In particular, the pro-inflammatory 13-hydroxyoctadecadienoic acid and its derived anti-inflammatory 13-hydroperoxoctadecaienoic acid were found lower and higher, respectively, in PBE+AAPH treated cells than AAPH treatment. Data from the present study support in vivo future experimental use of pomegranate by-product extract to study its potential beneficial effect against oxidative stress conditions in dairy cattle.

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Section: Bovine Aortic Endothelial Cell Isolation and Culturingmentioning

confidence: 99%

Pomegranate (Punica granatum) By-Product Extract Influences the Oxylipids Profile in Primary Bovine Aortic Endothelial Cells in a Model of Oxidative Stress

et al. 2022

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“…Bovine aortic endothelial cells (BAEC) were collected from the aorta of healthy Holstein dairy cows based on techniques previously described by Sordillo et al (1998). The BAEC were purified by dilution cloning techniques and then cultured in Ham's F-12K medium containing 10% fetal bovine serum (FBS), 20 mM HEPES, 2 mM l-glutamine, 1% (vol/vol) antibioticantimycotic, heparin (100 μg/mL), insulin (10 μg/mL), transferrin (5 μg/mL), and sodium selenite (10 ng/ mL).…”

Section: Bovine Aortic Endothelial Cell Isolation and Culturingmentioning

confidence: 99%

Evaluation of natural plant extracts as antioxidants in a bovine in vitro model of oxidative stress

Ciampi

Sordillo

Gandy

et al. 2020

Journal of Dairy Science

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Oxidative stress contributes to many inflammatorybased diseases of dairy cattle especially during periods of increased metabolic activity such as around calving. Endothelial cells play a key role in maintaining normal inflammatory responses, but they are especially susceptible to macromolecule damage during times of oxidative stress. Therefore, bovine aortic endothelial cells (BAEC) were used to study the effect of natural tanninbased extracts on oxidative stress that may improve health and well-being of cattle. Tannins are secondary metabolites in plants with potent antioxidant activity that have been used as natural feed additives for foodproducing animals. However, there is little information on how tannin-rich plant extracts may affect oxidative stress in dairy cattle. The objective of this study was to evaluate the antioxidant effect of pomegranate (Punica granatum; PMG), tara (Caesalpinia spinosa; TA), chestnut (Castanea sativa; CH), and gambier (Uncaria gambir; GM) natural extracts using an in vitro BAEC model of oxidative stress. Natural extracts were tested at a concentration of 80 μg/mL. Viability, apoptosis, intracellular reactive oxygen species, and isoprostanes were determined on cultured BAEC treated with different plant natural extracts. No changes in cell viability was detected following PMG and GM treatments. In contrast, there was a 30% reduction of BAEC viability following treatment with CH or TA extracts. Intracellular reactive oxygen species production was significantly less abundant in cells treated with natural extracts than with the lipopolysaccharide control. Moreover, antioxidant activity varied according to the tested extract, showing a reduction of 63, 45, 51, and 27% in PMG, GM, CH, and TA, respectively. The formation of isoprostanes as a consequence of lipid peroxidation after induction of oxidative stress also were significantly decreased in PMG-treated cells when compared with the untreated cells. Theses findings suggest that PMG extract has the potential to mitigate oxidative stress without detrimental effects on cell viability. Further in vitro and in vivo research is warranted to explore the antioxidant potential of PMG extract as a dietary supplement to control oxidative stress in dairy cattle.

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Increased neutrophil adherence and adhesion molecule mRNA expression in endothelial cells during selenium deficiency

Maddox

Aherne

Reddy

et al. 1999

Journal of Leukocyte Biology

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Leukocyte aggregation and activation on endothelial cells (EC) are important preliminary events in leukocyte migration into tissue and subsequent inflammation. Thus, an increase in leukocyte adherence has the potential to affect inflammatory disease outcome. Selenium (Se) is an integral part of the antioxidant enzyme glutathione peroxidase (GSH-Px) and plays an important role in the maintenance of the redox state of a cell. Se supplementation in the bovine has been shown to improve the outcome of acute mastitis caused by coliform bacteria, in part by enhancing the speed of neutrophil migration into the affected mammary gland. However, the mechanisms by which Se modulates neutrophil migration have not been elucidated. Therefore, an in vitro model of Se deficiency in primary bovine mammary artery EC was used to examine the impact of Se status on the adhesive properties of EC. The effect of Se on functional activities was examined by measuring neutrophil adherence to Se-deficient and Se-supplemented EC. Se-deficient EC showed significantly enhanced neutrophil adherence when stimulated with tumor necrosis factor ␣ (TNF-␣) for 4 or 24 h, interleukin-1 for 12 h, or H 2 O 2 for 20 min (P F 0.05). To determine the mechanisms underlying these changes in neutrophil adherence, the expression of EC adhesion molecules, ICAM-1, E-selectin, and P-selectin were examined at the molecular level by a competitive reverse transcription-polymerase chain reaction. Results revealed higher mRNA expression for Eselectin and ICAM-1 in Se-deficient EC stimulated with TNF-␣ for 3 and 6 h, and greater expression of P-selectin mRNA in Se-supplemented EC with 3-h TNF-␣ stimulation. These studies provide new information to establish the role of Se nutrition in the initiation of leukocyte adherence to endothelium.

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Untitled

Cited by 21 publications

References 13 publications

Pomegranate (Punica granatum) By-Product Extract Influences the Oxylipids Profile in Primary Bovine Aortic Endothelial Cells in a Model of Oxidative Stress

Pomegranate (Punica granatum) By-Product Extract Influences the Oxylipids Profile in Primary Bovine Aortic Endothelial Cells in a Model of Oxidative Stress

Evaluation of natural plant extracts as antioxidants in a bovine in vitro model of oxidative stress

Increased neutrophil adherence and adhesion molecule mRNA expression in endothelial cells during selenium deficiency

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