Increased neutrophil adherence and adhesion molecule mRNA expression in endothelial cells during selenium deficiency

Abstract

Leukocyte aggregation and activation on endothelial cells (EC) are important preliminary events in leukocyte migration into tissue and subsequent inflammation. Thus, an increase in leukocyte adherence has the potential to affect inflammatory disease outcome. Selenium (Se) is an integral part of the antioxidant enzyme glutathione peroxidase (GSH-Px) and plays an important role in the maintenance of the redox state of a cell. Se supplementation in the bovine has been shown to improve the outcome of acute mastitis caused by coliform bacteria, in part by enhancing the speed of neutrophil migration into the affected mammary gland. However, the mechanisms by which Se modulates neutrophil migration have not been elucidated. Therefore, an in vitro model of Se deficiency in primary bovine mammary artery EC was used to examine the impact of Se status on the adhesive properties of EC. The effect of Se on functional activities was examined by measuring neutrophil adherence to Se-deficient and Se-supplemented EC. Se-deficient EC showed significantly enhanced neutrophil adherence when stimulated with tumor necrosis factor ␣ (TNF-␣) for 4 or 24 h, interleukin-1 for 12 h, or H 2 O 2 for 20 min (P F 0.05). To determine the mechanisms underlying these changes in neutrophil adherence, the expression of EC adhesion molecules, ICAM-1, E-selectin, and P-selectin were examined at the molecular level by a competitive reverse transcription-polymerase chain reaction. Results revealed higher mRNA expression for Eselectin and ICAM-1 in Se-deficient EC stimulated with TNF-␣ for 3 and 6 h, and greater expression of P-selectin mRNA in Se-supplemented EC with 3-h TNF-␣ stimulation. These studies provide new information to establish the role of Se nutrition in the initiation of leukocyte adherence to endothelium.

Selenium-Dependent Regulation of Oxidative Stress and Immunity in Periparturient Dairy Cattle

“…The selectin family of adhesion molecules, including E-selectin and P-selectin, are primarily responsible for the initial phase of the leukocyte adhesion cascade. The Ig superfamily of adhesion molecules includes intercellular adhesion molecule-1 (ICAM1), vascular adhesion molecule-1 (VCAM1), and platelet endothelial adhesion molecule-1 (PECAM1) [24]. The expression of both ICAM1 and VCAM1 increase significantly during inflammation and is involved in the firm attachment of blood leukocytes to vascular endothelial cells [18].…”

Micronutrients and immune function in cattle

“…In goats both random and chemotactic migration of neutrophils in vitro were reduced by Se deficiency (Aziz et al 1984). Recently, bovine mammary endothelial cells grown in Se-deficient cell culture media were found to exhibit enhanced neutrophil adherence when stimulated with TNF-α and IL-2 (Maddox et al 1999). This factor could affect neutrophil migration into tissues and subsequent inflammation.…”

Proteomics of inflammatory and oxidative stress response in cows with subclinical and clinical mastitis

“…Indirectly, ROS can damage cellular components and membranes and thus modify metabolic pathways [22]. Oxidative stress can enhance the adhesion of activated neutrophils to mammary endothelial cells and intensify inflammation [23]. Several studies have shown that supplementing dairy cows with an adequate level of antioxidants increase immune function of cows, i.e.…”