A 3D printed <scp>TCP</scp>/<scp>HA</scp> structure as a new osteoconductive scaffold for vertical bone augmentation

Carrel, Jean-Pierre; Wiskott, Anselm; Moussa, Mira; Rieder, Peter; Scherrer, Susanne S.; Durual, Stéphane

doi:10.1111/clr.12503

Cited by 90 publications

(105 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Bai et al [54] and Lin et al [55] tested the combination of rhBMP-2 and VEGF, which seems to accelerate bone healing. Different carrier materials [2, 5, 34, 56, 57] have been examined but the ultimate carrier is yet to be found. ICBM itself has shown bone regenerative capacity in combination with embryonal stem cells [58, 59].…”

Section: Discussionmentioning

confidence: 99%

Vertical bone regeneration using rhBMP-2 and VEGF

et al. 2017

View full text Add to dashboard Cite

BackgroundSufficient vertical and lateral bone supply and a competent osteogenic healing process are prerequisities for the successful osseointegration of dental implants in the alveolar bone. Several techniques including autologous bone grafts and guided bone regeneration are applied to improve quality and quantity of bone at the implantation site. Depending on the amount of lacking bone one- or two-stage procedures are required. Vertical bone augmentation has proven to be a challenge particularly in terms of bone volume stability. This study focuses on the three dimensional vertical bone generation in a one stage procedure in vivo. Therefore, a collagenous disc-shaped scaffold (ICBM = Insoluble Collagenous Bone Matrix) containing rhBMP-2 (Bone Morphogenetic Protein-2) and/or VEGF (Vascular Endothelial Growth Factor) was applied around the coronal part of a dental implant during insertion. RhBMP-2 and VEGF released directly at the implantation site were assumed to induce the generation of new vertical bone around the implant.MethodsOne hundred eight titanium implants were inserted into the mandible and the tibia of 12 mini pigs. Four experimental groups were formed: Control group, ICBM, ICBM + BMP-2, and ICBM + BMP-2 + VEGF.After 1, 4 and 12 weeks the animals were sacrificed and bone generation was investigated histologically and histomorphometrically.ResultsAfter 12 weeks the combination of ICBM + rhBMP2 + VEGF showed significantly more bone volume density (BVD%), a higher vertical bone gain (VBG) and more vertical bone gain around the implant (PVBG) in comparison to the control group.ConclusionBy using collagenous disc-shaped matrices in combination with rhBMP-2 and VEGF vertical bone can be generated in a one stage procedure without donor site morbidity. The results of the presenting study suggest that the combination of rhBMP-2 and VEGF applied locally by using a collagenous carrier improves vertical bone generation in vivo. Further research is needed to establish whether this technique is applicable in clinical routines.

show abstract

Section: Discussionmentioning

confidence: 99%

Vertical bone regeneration using rhBMP-2 and VEGF

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Bone tissue engineering aims to create new functional bone via synergistic therapy using three biological elements 1) (cells [2][3][4] , signaling molecules [5][6][7][8] , and natural and artificial scaffolds [9][10][11] ) in order to reverse bone loss caused by infectious disease [12][13][14][15] , trauma 16) , and cancer 17) or to augment host bone for dental implants 18,19) . Application of a three-dimensional (3D) scaffold to the bone defect produces a regenerative space for tissue reconstruction and stimulates cell proliferation and differentiation, along with angiogenesis and extracellular matrix secretion 20) .…”

Section: Introductionmentioning

confidence: 99%

Dose effects of beta-tricalcium phosphate nanoparticles on biocompatibility and bone conductive ability of three-dimensional collagen scaffolds

et al. 2017

View full text Add to dashboard Cite

Three-dimensional collagen scaffolds coated with beta-tricalcium phosphate (β-TCP) nanoparticles reportedly exhibit good bioactivity and biodegradability. Dose effects of β-TCP nanoparticles on biocompatibility and bone forming ability were then examined. Collagen scaffold was applied with 1, 5, 10, and 25 wt% β-TCP nanoparticle dispersion and designated TCP1, TCP5, TCP10, and TCP25, respectively. Compressive strength, calcium ion release and enzyme resistance of scaffolds with β-TCP nanoparticles applied increased with β-TCP dose. TCP5 showed excellent cell-ingrowth behavior in rat subcutaneous tissue. When TCP10 was applied, osteoblastic cell proliferation and rat cranial bone augmentation were greater than for any other scaffold. The bone area of TCP10 was 7.7-fold greater than that of non-treated scaffold. In contrast, TCP25 consistently exhibited adverse biological effects. These results suggest that the application dose of β-TCP nanoparticles affects the scaffold bioproperties; consequently, the bone conductive ability of TCP10 was remarkable.

show abstract

“…Bone substitutes are used in order to fill, rebuild or replace bone defects (Carrel et al, 2014;Draenert, Huetzen, Neff, & Mueller, 2014). There is an intense search by the medical and dental fields for materials that can replace lost bone tissue with consequent anatomical and functional recovery of the tissue or organ.…”

Section: Introductionmentioning

confidence: 99%

“…There is an intense search by the medical and dental fields for materials that can replace lost bone tissue with consequent anatomical and functional recovery of the tissue or organ. Autogenous bone, despite being considered the gold standard for use in grafts, has limited use because it causes damage at the donor site and presents qualities related to age and general health of the individual, besides being exhaustible (Carrel et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

<b>Analysis of indicators of osteogenesis, cytotoxicity and genotoxicity of an experimental β-TCP compared to other bone substitutes

Milhan

Carvalho

Prado

et al. 2017

Acta Sci. Health Sci.

View full text Add to dashboard Cite

ABSTRACT. The aim of this study was to evaluate the indicators of osteogenesis, cytotoxicity and genotoxicity of an experimental beta tri-calcium phosphate (experimental β-TCP) compared with two other bone substitutes: bovine hydroxyapatite (HA) (Bio-Oss ® -Geistlich) and beta tri-calcium phosphate (β-TCP -Bionnovation). The cell viability and genotoxicity were measured by MTT and MNT assay, respectively. The indicators of osteogenesis were analyzed by alkaline phosphatase activity, total protein content, and calcium deposition. The MTT and MNT assay showed that none of the tested materials was cytotoxic nor genotoxic. Concerning the indicators of osteogenesis, it was observed that cells in contact with all the materials were able to induce the osteogenesis and this process was influenced by the period of the cell culture in contact with bone substitutes. Based on the results of this study, it was concluded that this experimental β-TCP appears to be a promising material as a bone substitute.Keywords: Beta tri-calcium phosphate, bovine hydroxyapatite.Análise dos indicadores da osteogênese, citotoxicidade e genotoxicidade de um β-TCP experimental comparado a outros substitutos ósseos RESUMO. O objetivo deste estudo foi avaliar os indicadores da osteogênese, citotoxicidade e genotoxicidade de um beta-tricálcio fosfato (β-TCP experimental) comparado com dois outros substitutos ósseos : Hidroxiapatita Bovina (HA) (Bio-Oss ® -Geistlich) e beta-tricálcio fosfato (β-TCPBionnovation). A viabilidade celular e genotoxicidade foram mensuradas pelos ensaios MTT e MNT, respectivamente. Os indicadores da osteogênese foram analisados pela atividade de fosfatase alcalina (ALP), conteúdo de proteína total, e deposição de cálcio. Os ensaios MTT e MNT mostraram que nenhum dos materiais testados foi citotóxico ou genotóxico. Em relação aos indicadores da osteogênese, foi observado que as células em contato com todos os materiais foram capazes de induzir a osteogênese, e que esse processo foi influenciado pelo período da cultura celular em contato com os substitutos ósseos. Baseado nos resultados desse estudo, conclui-se que este β-TCP experimental parece ser um material promissor para ser utilizado como substituto ósseo.Palavras-chave: Beta-tricálcio fosfato, Hidroxiapatita Bovina.

show abstract

A 3D printed TCP/HA structure as a new osteoconductive scaffold for vertical bone augmentation

Cited by 90 publications

References 20 publications

Vertical bone regeneration using rhBMP-2 and VEGF

Vertical bone regeneration using rhBMP-2 and VEGF

Dose effects of beta-tricalcium phosphate nanoparticles on biocompatibility and bone conductive ability of three-dimensional collagen scaffolds

<b>Analysis of indicators of osteogenesis, cytotoxicity and genotoxicity of an experimental β-TCP compared to other bone substitutes

Contact Info

Product

Resources

About