A bacterial infection by <i>Vibrio harveyi</i> causing heavy reduction of cultured lined seahorse <i>Hippocampus erectus</i>

Qin, Geng; Wang, X; Tan, Shuo; Lin, Qiang

doi:10.1111/jfd.12533

Cited by 23 publications

(20 citation statements)

References 19 publications

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“…Seahorses have been in great demand due to their medicinal values, although they are mainly supplied by field fishing. Nevertheless, large-scale aquaculture of seahorses has been seriously limited by fatal diseases, which are usually caused by exogenous pathogens, such as vibrio [9][10][11] and ciliate [12]. Enteritis is a serious disease restricting the practical aquaculture of the lined seahorse with strong infectiousness and high fatality rates [11,13], especially occurring most frequently among juveniles during a short period from the newborn to approximately one month after birth.…”

Section: Introductionmentioning

confidence: 99%

High-Throughput Identification of Putative Antimicrobial Peptides from Multi-Omics Data of the Lined Seahorse (Hippocampus erectus)

Chen

You

et al. 2019

Marine Drugs

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Lined seahorse (Hippocampus erectus), the most widely cultivated seahorse in China, has been in short supply because of its important medicinal value; meanwhile, unnatural deaths caused by various diseases (especially enteritis) have limited their practical large-scale aquaculture. Antimicrobial peptides (AMPs), as the best alternative to antibiotics, have been extensively applied in agricultural practices. In this study, we identified 290 putative AMP sequences from our previously published genome and transcriptome data of the lined seahorse. Among them, 267 are novel, and 118 were validated by our proteome data generated in the present study. It seems that there is a tissue preference in the distribution of AMP/AMP precursor transcripts, such as lectins in the male pouch. In addition, their transcription levels usually varied during development. Interestingly, the representative lectins kept extremely high levels at the pre-pregnancy stage while at relatively lower levels at other stages. Especially Lectin25, with the highest transcription levels and significant developmental changes, has been reported to be involved in seahorse and human pregnancy. The comparison of transcriptome data between one-day and three-month juveniles indicated that Hemoglobin2 (Hemo2) was significantly upregulated in the body, haslet, and brain. Our proteome data of female and male individuals revealed three putative AMP precursors with sexual specificity, including two male-biased cyclin-dependent kinases (CDK-like16 and CDK-like23) and one female-biased bovine pancreatic trypsin inhibitor 2 (BPTI2). In conclusion, our present high-throughput identification of putative AMP sequences from multi-omics (including genomics, transcriptomics, and proteomics) data provides an overview of AMPs in the popular lined seahorse, which lays a solid foundation for further development of AMP-based fish food additives and human drugs.

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Section: Introductionmentioning

confidence: 99%

High-Throughput Identification of Putative Antimicrobial Peptides from Multi-Omics Data of the Lined Seahorse (Hippocampus erectus)

Chen

You

et al. 2019

Marine Drugs

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“…However, due to habitat destruction and heavy trade, wild seahorse populations have greatly declined in recent decades (Lourie, Pollom, & Foster, ). Seahorse aquaculture has become an important and potential candidate for addressing the high market demand (Qin, Wang, Tan, & Lin, ). However, widespread diseases are considered an important bottleneck for seahorse culturing and represent a serious challenge for scientific research in recent years (Declercq et al., ; Wang, Zhang, Qin, Luo, & Lin, ).…”

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confidence: 99%

“…This study is the first to confirm that V. vulnificus infections can induce high mortality in seahorses. Previous reports have shown that V. harveyi, V. fortis , V. parahaemolyticus, V. splendidus and V. alginolyticus may also be involved in seahorse diseases (Alcaide et al., ; Balcazar, Gallo‐Bueno, Planas, & Pintado, ; Lin, Zhang et al., ; Martins et al., ; Qin et al., ; Tendencia, ; Wang et al., ). V. vulnificus often causes large and disfiguring ulcers in fishes (Oliver, ), and co‐infection by V. vulnificus and scuticociliate in seahorses might aggravate the extent to which damage occurs.…”

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confidence: 99%

“…The lined seahorse (Hippocampus erectus) has been the most popular cultured species in China over the past 10 years (Lin et al, 2012); however, these animals have been impacted by frequent disease events (Lin, Zhang, Liu, & Xiao, 2016;Qin et al, 2017;Wang et al, 2016). We first distinguished scuticociliate-infected seahorses in 2012.…”

mentioning

confidence: 99%

“…The neighbour-joining (NJ) tree analysis based on 16sRNA sequences showed that the isolates in diseased seahorses were Vibrio vulnificus strains (Figure 1d) (Alcaide et al, 2001;Balcazar, Gallo-Bueno, Planas, & Pintado, 2010;Martins et al, 2010;Qin et al, 2017;Tendencia, 2004;Wang et al, 2016). V. vulnificus often causes large and disfiguring ulcers in fishes (Oliver, 2005), and co-infection by V. vulnificus and scuticociliate in seahorses might aggravate the extent to which damage occurs.…”

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confidence: 99%

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Seahorse TLR5 gene responses to Vibrio vulnificus infection, which in combination with scuticociliates causes heavy reductions in seahorse aquaculture

Qin

Zhang

et al. 2018

Journal of Fish Diseases

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Vibrio harveyi: a serious pathogen of fish and invertebrates in mariculture

Zhang

Austin

2020

Mar Life Sci Technol

205

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Vibrio harveyi, which belongs to family Vibrionaceae of class Gammaproteobacteria, includes the species V. carchariae and V. trachuri as its junior synonyms. The organism is a well-recognized and serious bacterial pathogen of marine fish and invertebrates, including penaeid shrimp, in aquaculture. Diseased fish may exhibit a range of lesions, including eye lesions/blindness, gastro-enteritis, muscle necrosis, skin ulcers, and tail rot disease. In shrimp, V. harveyi is regarded as the etiological agent of luminous vibriosis in which affected animals glow in the dark. There is a second condition of shrimp known as Bolitas negricans where the digestive tract is filled with spheres of sloughed-off tissue. It is recognized that the pathogenicity mechanisms of V. harveyi may be different in fish and penaeid shrimp. In shrimp, the pathogenicity mechanisms involved the endotoxin lipopolysaccharide, and extracellular proteases, and interaction with bacteriophages. In fish, the pathogenicity mechanisms involved extracellular hemolysin (encoded by duplicate hemolysin genes), which was identified as a phospholipase B and could inactivate fish cells by apoptosis, via the caspase activation pathway. V. harveyi may enter the so-called viable but nonculturable (VBNC) state, and resuscitation of the VBNC cells may be an important reason for vibriosis outbreaks in aquaculture. Disease control measures center on dietary supplements (including probiotics), nonspecific immunostimulants, and vaccines and to a lesser extent antibiotics and other antimicrobial compounds.

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A bacterial infection by Vibrio harveyi causing heavy reduction of cultured lined seahorse Hippocampus erectus

Cited by 23 publications

References 19 publications

High-Throughput Identification of Putative Antimicrobial Peptides from Multi-Omics Data of the Lined Seahorse (Hippocampus erectus)

High-Throughput Identification of Putative Antimicrobial Peptides from Multi-Omics Data of the Lined Seahorse (Hippocampus erectus)

Seahorse TLR5 gene responses to Vibrio vulnificus infection, which in combination with scuticociliates causes heavy reductions in seahorse aquaculture

Vibrio harveyi: a serious pathogen of fish and invertebrates in mariculture

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