A barley gene family homologous to the maize rust resistance gene Rp1-D

Rostoks, Nils; Zale, Janice; Soule, JD; Brueggeman, Robert; Druka, Arnis; Kudrna, David; Steffenson, Brian J.; Kleinhofs, A.

doi:10.1007/s00122-002-0902-8

Cited by 17 publications

(10 citation statements)

References 44 publications

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“…In the region spanning the pic20 and Rpg1 loci (Fig. 2C), the ratio of physical to genetic distance for six marker intervals was found to vary from 350 kb/cM to greater than 25 Mb/cM Rostoks et al 2002). Clearly, the physical distances of ssCH4 and MWG851 from Rdg2a can only be determined empirically.…”

Section: Discussionmentioning

confidence: 95%

“…3). RGA RFLP probes comprised b9 (Leister et al 1999), ssCH4 (also called Rlch4;Seah et al 1998), ABG1019 (Brueggeman et al 2001), pic20C (as defined by Rostoks et al 2002), and the barley EST BE216309 which has high homology to S-9202. The relative positions determined for the RGAs (Fig.…”

Section: Selection Of Recombinants For Fine Mappingmentioning

confidence: 99%

“…ABG1019 showed significant similarity to NBS-LRR resistance proteins in a BLASTX search and was mapped to barley chromosome 7H bin 01 in the SteptoeMorex mini mapper population (Brueggeman and Kleinhofs, unpublished data). The pic20 probe was derived from the most proximal member of the pic20 RGA gene cluster on 7H (pic20C; Rostoks et al 2002) by amplifying a 724 bp section of the gene (nucleotides 63 to 786 of AF414173) from Thibaut genomic DNA. The fragment identity was confirmed by sequencing.…”

Section: Rga Clones Used To Make Rflp Probesmentioning

confidence: 99%

“…Several classes of RGAs, which are unrelated to one another at the DNA level, have been mapped to the Rdg2a region. These include pic20 (Ayliffe et al 2000;Rostoks et al 2002), Hvb9 ), ssCH4 (Seah et al 1998), ABG1019 , MWG851 ) and the highly similar BE216309 (Kleinhofs laboratory, http://barleygenomics.wsu.edu/) and S-9202 . This region also contains the stem rust resistance gene Rpg1 encoding a novel receptorkinase-like resistance protein (Brueggemann et al 2002).…”

Section: Introductionmentioning

confidence: 98%

“…The region immediately surrounding Rpg1 shows a high level of synteny with a region on the short arm of rice chromosome 6, although a Rpg1 homologue is absent from this region (and the whole rice genome; Kilian et al 1997;Han et al 1999;Rostoks et al 2002).…”

Section: Introductionmentioning

confidence: 99%

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High-resolution genetic mapping of the leaf stripe resistance gene Rdg2a in barley

et al. 2003

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The dominant gene Rdg2a of barley conferring resistance to the hemi-biotrophic seed-borne pathogen Pyrenophora graminea is located in the distal region of chromosome arm 1 (7H)S. As the first step towards isolating the gene, a high-resolution genetic map of the region was constructed using an F(2) population of 1,400 plants (Thibaut Rdg2axMirco). The map included six classes of resistance gene analogues (RGAs) tightly associated with Rdg2a. Rdg2a was delimited to a genetic interval of 0.14 cM between the RGAs ssCH4 and MWG851. Additional markers were generated using the sequence from the corresponding region on rice chromosome 6, allowing delimitation of the Rdg2a syntenic interval in rice to a 115 kbp stretch of sequence. Analysis of the rice sequence failed to reveal any genes with similarity to characterized resistance genes. Therefore, either the rice-barley synteny is disrupted in this region, or Rdg2a encodes a novel type of resistance protein.

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Section: Discussionmentioning

confidence: 95%

Section: Selection Of Recombinants For Fine Mappingmentioning

confidence: 99%

Section: Rga Clones Used To Make Rflp Probesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

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High-resolution genetic mapping of the leaf stripe resistance gene Rdg2a in barley

et al. 2003

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Maize

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2006

Cereals and Millets

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Homologues of the maize rust resistance gene Rp1-D are genetically associated with a major rust resistance QTL in sorghum

et al. 2004

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As part of a comparative mapping study between sugarcane and sorghum, a sugarcane cDNA clone with homology to the maize Rp1-D rust resistance gene was mapped in sorghum. The cDNA probe hybridised to multiple loci, including one on sorghum linkage group (LG) E in a region where a major rust resistance QTL had been previously mapped. Partial sorghum Rp1-D homologues were isolated from genomic DNA of rust-resistant and -susceptible progeny selected from a sorghum mapping population. Sequencing of the Rp1-D homologues revealed five discrete sequence classes: three from resistant progeny and two from susceptible progeny. PCR primers specific to each sequence class were used to amplify products from the progeny and confirmed that the five sequence classes mapped to the same locus on LG E. Cluster analysis of these sorghum sequences and available sugarcane, maize and sorghum Rp1-D homologue sequences showed that the maize Rp1-D sequence and the partial sugarcane Rp1-D homologue were clustered with one of the sorghum resistant progeny sequence classes, while previously published sorghum Rp1-D homologue sequences clustered with the susceptible progeny sequence classes. Full-length sequence information was obtained for one member of a resistant progeny sequence class ( Rp1-SO) and compared with the maize Rp1-D sequence and a previously identified sorghum Rp1 homologue ( Rph1-2). There was considerable similarity between the two sorghum sequences and less similarity between the sorghum and maize sequences. These results suggest a conservation of function and gene sequence homology at the Rp1 loci of maize and sorghum and provide a basis for convenient PCR-based screening tools for putative rust resistance alleles in sorghum.

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A barley gene family homologous to the maize rust resistance gene Rp1-D

Cited by 17 publications

References 44 publications

High-resolution genetic mapping of the leaf stripe resistance gene Rdg2a in barley

High-resolution genetic mapping of the leaf stripe resistance gene Rdg2a in barley

Maize

Homologues of the maize rust resistance gene Rp1-D are genetically associated with a major rust resistance QTL in sorghum

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