A Bio-Barcode Electrochemical DNA Biosensor Based on Poly T30 Copper Nanoparticle Signaling

Abstract

Simple, cost effective, high sensitive and selective detection strategies for disease related DNA sequences in clinical diagnostics and research purposes are still on demand. Detection of DNA specific sequences of particular biomedical importance, based on electrochemical signaling, has been reported as a promising analytical approach for medical diagnostics due to its simplicity, cost effective, sensitivity, selectivity and rapidity. Herein, a simple and cost effective DNA biosensor based on poly T30 Copper Nanoparticle Signaling and biobarcode technique is presented for the first time. In this design, complementary sequence places the poly T30 modified bio-barcode probe (P2-SiO2-T30) on the sensor interface. Upon copper reduction reaction, copper nanoparticles (CuNPs) are clustered along the poly T30 modified bio-barcode probe (P2-SiO2-T30-CuNPs). During electrochemical measurements copper nanoparticles (CuNPs) are oxidized to give current signal. This detection strategy has a detection limit of 10 pM.

3-Mercaptopropyl Trimethoxysilane @ Gadolinium Oxide Nanoprobes: An Effective Fluorescence-Sensing Platform for Cysteine

“…The spiking was performed with three different concentrations, i.e., 10, 25 and 50 µm concentrations of Cys in the serum solution. The emission spectra were recorded for each spiked amount of Cys [61][62][63][64][65]. All the prepared samples showed a recovery of around 95 to 101% of Cys in the presence of serum samples (Table 2).…”

Preparation of Copper Nanoplates in Aqueous Phase and Electrochemical Detection of Dopamine

“…Among the numerous metallic nanomaterials such as silver and gold, copper (Cu) is one thousand times more abundant than silver in the earth’s crust, it is one hundred times cheaper than silver, and it is six thousand times cheaper than gold. In recent decades, Cu has received considerable attention due to its inexpensiveness, high abundance, good electrical conductivity, and wide application in various fields, such as catalysis (4-nitrophenol reduction, electrocatalytic oxygen reduction, and electrocatalytic carbon dioxide reduction), as antibacterial agents, in electronic devices (electronic ink and flexible transparent electrodes), and in electrochemical sensors (hydrogen peroxide, glucose, and dopamine) [ 1 , 2 , 3 , 4 , 5 , 6 , 7 ]. The performance of nanoparticles in various applications greatly depends on their morphologies and surface chemistries [ 8 , 9 , 10 , 11 , 12 ].…”

Fabrication and Characterization of Acute Myocardial Infarction Myoglobin Biomarker Based on Chromium-Doped Zinc Oxide Nanoparticles

“…Electrochemical sensors/transducers being low cost, fast and efficient have been extensively researched [ 8 , 9 , 10 , 11 ]. Moreover, nanomaterials-based sensors show the potential for economically viable solutions to cut down time consumption and costs of early detection and cures for cardiovascular diseases [ 12 , 13 , 14 , 15 , 16 , 17 , 18 ].…”

“…Size compatibility of nanomaterials with biomolecules leads to better immobilization over the surface of nanostructured materials [ 14 , 15 , 16 ]. Nanomaterials-based biosensors are intriguing due to their potential for detecting sub-nanomolar concentrations using a few microliters of volume [ 17 , 18 ]. Nanomaterials-based biosensors are illustrated as potential replacements for conventional devices with high sensitivity, rapid response, and low detection limit [ 6 , 21 , 22 ].…”