A bio-sensing strategy for the detection of prions in foods

Anand, Ashish; Moreira, Rosana G.; Henry, J. E.; Chowdhury, Mustafa H.; Cote, Gerald L.; Good, Theresa A.

doi:10.1016/j.lwt.2004.09.015

Cited by 6 publications

(4 citation statements)

References 13 publications

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“…Addition of Sodium Dodecyl Sulfate (SDS) at 0.3 mg mL -1 to gelatin samples facilitated detection in gelatin. The lowest detectable concentration of prion in gelatin was 0.5nmol L -1 [32].…”

Section: Biosensor Based Methodsmentioning

confidence: 90%

Molecular Nanobiotechnological Approaches for the Detection and Therapy of Prion Related Diseases

P¹,

Thampy²,

Venugopalan³

et al. 2012

Nano BioMed ENG

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Prion diseases are associated with the accumulation in the brain of an abnormal, protease resistant isoform of a host encoded glycoprotein known as prion protein (PrP). Nanotechnology in combination with biotechniques promises a broad spectrum of highly innovative approaches for overcoming the challenges posed by the prions. Recent advances in molecular nanobiotechnology have brought in the potential of molecular targeting in diagnosis and therapies of various diseases. Their high binding sensitivity and specificity added by their small size have favored the identification by in vitro protocols. Molecular targeting has initiated exciting technologies based on conjugation of biomolecules to nanoparticles. This review article is an extensive study of various research oriented nanobiotechnological protocols for rapid identification and cure for prion diseases both at in vivo and in vitro options.

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Section: Biosensor Based Methodsmentioning

confidence: 90%

Molecular Nanobiotechnological Approaches for the Detection and Therapy of Prion Related Diseases

P¹,

Thampy²,

Venugopalan³

et al. 2012

Nano BioMed ENG

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“…The supramolecular assembly of different amphiphilic molecules allows for the incorporating of sensors into vesicular aggregates with high colloid stability and biocompatibility, which provides a good basis for creating sensory systems [ 1 , 2 , 3 , 4 , 5 ]. The uses of colorimetric and luminescence techniques for sensing are particularly attractive since they can facilitate the naked-eye or simple spectroscopic detection of biomolecules or water-soluble toxicants [ 6 , 7 , 8 , 9 , 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

Supramolecular Optimization of Sensory Function of a Hemicurcuminoid through Its Incorporation into Phospholipid and Polymeric Polydiacetylenic Vesicles: Experimental and Computational Insight

et al. 2023

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This work presents the synthesis of a new representative of hemicurcuminoids with a nonyloxy substituent (HCur) as a fluorescent amphiphilic structural element of vesicular aggregates based on phosphatidylcholine (PC), phosphatidylserine (PS), and 10,12-pentacosadiynoic acid (PCDA). Both X-ray diffraction analysis of the single crystal and 1H NMR spectra of HCur in organic solvents indicate the predominance of the enol-tautomer of HCur. DFT calculations show the predominance of the enol tautomer HCur in supramolecular assemblies with PC, PS, and PCDA molecules. The results of the molecular modeling show that HCur molecules are surrounded by PC and PS with a rather weak exposure to water molecules, while an exposure of HCur molecules to water is enhanced under its supramolecular assembly with PCDA molecules. This is in good agreement with the higher loading of HCur into PC(PS) vesicles compared to PCDA vesicles converted into polydiacetylene (PDA) ones by photopolymerization. HCur molecules incorporated into HCur-PDA vesicles exhibit greater planarity distortion and hydration effect in comparison with HCur-PC(PS) ones. HCur-PDA is presented as a dual fluorescence-chromatic nanosensor responsive to a change in pH within 7.5–9.5, heavy metal ions and polylysine, and the concentration-dependent fluorescent response is more sensitive than the chromatic one. Thus, the fluorescent response of HCur-PDA allows for the distinguishing between Cd2+ and Pb2+ ions in the concentration range 0–0.01 mM, while the chromatic response allows for the selective sensing of Pb2+ over Cd2+ ions at their concentrations above 0.03 mM.

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“…To overcome this limitation we designed a sensor based on the detection of a TNT-specific antibody. Particularly, the concentration of TNT in solution was measured by quantifying the antibody displacement from the surface by TNT, a strategy that allows amplifying the output signal compared to the signal associated with direct detection of TNT [22][23][24][25]. The detection of TNT using displacement assay was previously reported using Enzyme-Linked Immunosorbent Assay (ELISA) [26][27][28], which is used as comparison in this study.…”

Section: Introductionmentioning

confidence: 99%

Sensitive Detection of TNT using Competition Assay on Quartz Crystal Microbalance

Wang

AlMakhaita²,

Biswal³

et al. 2012

J Biosens Bioelectron

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Currently available TNT sensors are characterized by high sensitivity, but low specificity, which limits the detection of TNT in dirty environments. We report here a TNT sensor designed to measure the displacement of a TNT-specific antibody by quartz crystal microbalance (QCM). This sensor combines high sensitivity of detection (0.1 ng/mL) with the ability to distinguish TNT from molecules with similar chemical properties. Particularly, the reliability of this method for the detection of TNT in dirty environments was investigated by using fertilizer solution and artificial seawater. Instead of measuring actual binding of TNT, the method described is based on the displacement of an anti-TNT antibody, which allows quantifying the concentration of TNT in solution with higher sensitivity. In addition, by utilizing the rate of antibody displacement, the detection time is significantly decreased from hours, which would be necessary to measure the frequency change at equilibrium, to minutes. A Langmuir kinetic model was used to describe the molecular interactions on the surface of the sensor and to establish a standard curve to estimate on-site TNT detection. In summary, QCM detection of anti-TNT antibody displacement provides a method for rapid detection of TNT with high sensitivity and specificity.

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A bio-sensing strategy for the detection of prions in foods

Cited by 6 publications

References 13 publications

Molecular Nanobiotechnological Approaches for the Detection and Therapy of Prion Related Diseases

Molecular Nanobiotechnological Approaches for the Detection and Therapy of Prion Related Diseases

Supramolecular Optimization of Sensory Function of a Hemicurcuminoid through Its Incorporation into Phospholipid and Polymeric Polydiacetylenic Vesicles: Experimental and Computational Insight

Sensitive Detection of TNT using Competition Assay on Quartz Crystal Microbalance

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