“…The successful immobilization of the β-glucosidase may have resulted from permanent crosslinking of the enzyme and carrier by EDC/NHS, as suggested by immobilization of the enzyme via strong covalent binding to CS-MIL-Fe. The enzyme efficacy was improved as the steric barriers surrounding the enzyme active sites were reduced due to the large surface area of the carrier and dispersion of the enzyme [ 33 , 34 ]. The robust chemical linkages between MOFs and enzymes are thought to involve covalent bonds [ 35 ].…”
“…The successful immobilization of the β-glucosidase may have resulted from permanent crosslinking of the enzyme and carrier by EDC/NHS, as suggested by immobilization of the enzyme via strong covalent binding to CS-MIL-Fe. The enzyme efficacy was improved as the steric barriers surrounding the enzyme active sites were reduced due to the large surface area of the carrier and dispersion of the enzyme [ 33 , 34 ]. The robust chemical linkages between MOFs and enzymes are thought to involve covalent bonds [ 35 ].…”
“…This immobilization efficiency at pH 6 is related to the ionizable groups of the enzyme which has more cationic groups at pH 6 than at pH 7 because its isoelectric point is 8.7 [ 26 ]. Changes in the ionic character of the enzyme surface can impact its interaction with the support surface, altering adsorption properties, orientation, or localized ionic repulsion/attraction [ 27 ]. Figure 1 Schematic representation for the synthesis of PET-hydrazide/GO and enzyme immobilization.…”
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
