A biocompatible model for evaluation of the responses of rat periapical tissue to a new zinc oxide-eugenol sealer

Abstract

We aimed to establish an experimental animal model to evaluate materials for endodontic therapy. We focused on the biocompatibility of new paste-type zinc oxide-eugenol (ZOE) sealer. The results of this sealer were compared with those of conventional powder/liquid ZOE and eugenol-free sealers. The molars of Wistar rats were extracted and repositioned in the original socket after application of the sealers on the root apices. Mild inflammation occurred in the periapical tissue of the replanted teeth with both ZOE sealers on day 7, whereas the eugenol-free sealer induced severe inflammation. On day 14, the lesions induced by all types of sealers were healed and replaced predominantly by fibrous connective tissue. Thus, all endodontic materials showed high biocompatibility, although the extent of inflammatory reactions during the early stages varied depending on the types of materials. We demonstrated that our animal model was useful for the assessment of the biocompatibility of endodontic materials.

Cytotoxicity Evaluation of Root Canal Sealers Using an In Vitro Experimental Model with Roots

“…Regarding the morphological changes of fibroblasts in the present study, the Endofill showed round-shaped fibroblasts with few processes and large acellular areas. Previous studies attributed the toxicity of ZOE-based cements to the eugenol and zinc ions released by the material in a wet environment (9,25). Therefore, one may suggest that the zinc and eugenol components released in the culture medium from the highly soluble cement Endofill may be responsible for higher cytotoxicity caused by this sealer to the cultured cells when compared to the other cements tested in the present investigation.…”

Biocompatibility of mineral trioxide aggregate and three new endodontic cements: An animal study

“…[2122] Rat subcutaneous implantation studies are acceptable experimental models for this assessment[23] and the inert nature of polyethylene tubes makes them suitable for implantation studies, so implantation of polyethylene or silicon tubes filled with endodontic materials into subcutaneous connective tissue of rats simulates in situ conditions of the materials. [24]…”

Evaluation of the biocompatibility of resin-based root canal sealers in rat periapical tissue

“…Ten microliters of these mixed materials were applied to each of the five root apices of M1 ( Fig. 1) using a 27-G needle (Terumo Company Limited, Tokyo, Japan) attached to a 1-mL plastic syringe 23) . The extracted tooth with materials was immediately repositioned in the original socket after the confirmation of hemostasis; these were classified as the "experimental groups".…”

“…The histomorphological parameters used in this study were based upon previously described criteria 23,25,26) . For the determination of scores, sections were evaluated according to four parameters.…”

“…However, there are few in vivo studies evaluating their biocompatibility in the periapical tissue. Thus, we aimed to evaluate the effects of the extrusion of resin-based RCSs such as Epiphany SE and AH Plus Jet and their biocompatibility in the periapical tissue using a recently established animal model for tooth replantation 23) .…”