A calcium-induced signaling cascade leading to osteogenic differentiation of human bone marrow-derived mesenchymal stromal cells

Barradas, Ana; Fernandes, Hugo; Gröen, Nathalie; Chai, Yoke Chin; Schrooten, Jan; Peppel, Jeroen van de; Leeuwen, Johannes P. T. M. van; Blitterswijk, Clemens A. van; Boer, Jan de

doi:10.1016/j.biomaterials.2012.01.020

Cited by 396 publications

(352 citation statements)

References 63 publications

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“…However, higher degradation rate and subsequent higher Ca 21 ion release can be considered as the key factor that pushes osteogenic differentiation. 57,59 Furthermore, previous studies conducted in the literature, have demonstrated such similar increase in ALP activity and intracellular calcium initiated with the incorporation of Fe 31 ion. [60][61][62][63] Based on ALP and intracellular calcium deposition, the ability of Fe-SeHA materials to support the osteogenic differentiation of hFOB cells can be suggested.…”

Section: Resultssupporting

confidence: 54%

Fe³⁺/⁻ dual doped nano hydroxyapatite: A novel material for biomedical applications

et al. 2017

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Dual ions substituted hydroxyapatite (HA) received attention from scientists and researchers in the biomedical field owing to their excellent biological properties. This paper presents a novel biomaterial, which holds potential for bone tissue applications. Herein, we have successfully incorporated ferric (Fe 31 )/selenate (SeO 22 4 ) ions into the HA structure (Ca 10-x-y Fe y (PO 4 ) 6-x (SeO 4 ) x (OH) 2-x-y O y ) (Fe-SeHA) through a microwave refluxing process. The Fe-SeHA materials were characterized by X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, and field emission scanning electron microscopy (FESEM). XRD and FTIR analyses revealed that Fe-SeHA samples were phase pure at 9008C. FESEM images showed that formation of rod-like shaped particles was inhibited dramatically with increasing Fe 31 amount. The Vickers hardness (HV) test showed that hardness values increased with increasing Fe 31 concentrations. Optical spectra of Fe-SeHA materials contained broadband over (200-600) nm. In vitro degradation and bioactivity tests were conducted in simulated body fluid (SBF). The incorporation of Fe 31 /SeO 22 4 ions into the HA structure resulted in a remarkably higher degradation rate along with intense growth of apatite granules on the surface of the Fe-SeHA discs with Ca/P ratio of 1.35-1.47. In vitro protein adsorption assay was conducted in fetal bovine serum (FBS) and it was observed that the adsorption of serum proteins on Fe-SeHA samples significantly increased with increasing Fe 31 concentration. In vitro cytotoxicity tests were performed with human fetal osteoblast (hFOB)

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Section: Resultssupporting

confidence: 54%

Fe³⁺/⁻ dual doped nano hydroxyapatite: A novel material for biomedical applications

et al. 2017

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“…We also found that the osteogenic differentiation of RMSCs on the composite scaffolds was largely enhanced as shown in Figure 3B and C. n-HA trapped in PLAGA microspheres might release certain amount of calcium ions before they reach equilibrium with the local microenvironment, this might play an important role during RMSC differentiation. A recent report by Boer et al found that free calcium ions in culture medium could trigger a calcium-inducing signaling pathway and lead to osteogenic differentiation of MSCs [31]. Thus, we speculate that the release of calcium ions from n-HA might be the driving force for the enhancement of RMSC differentiation towards osteoblasts.…”

Section: Cellular Response Of Plaga/n-ha Composite Scaffolds In Vitromentioning

confidence: 61%

Evaluation of PLAGA/n-HA Composite Scaffold Bioactivity in vitro

Lv¹,

Deng²,

Nair³

et al. 2014

Bioceram Dev Appl

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Polymeric sintered microsphere scaffolds have shown their tremendous potential in bone tissue engineering applications due to their highly porous and interconnected three dimensional structure and excellent mechanical properties. While these scaffolds are able to support basic cellular activity after seeding cells on them, the bioactivity of these scaffolds in terms of enhancing the biological performance of stem cells during bone regeneration is still under satisfactory. We hypothesized that incorporation of bioactive addictive such as hydroxyapatite into these scaffolds could improve their bioactivity without sacrificing the bulk properties of the scaffolds. We have successfully incorporated nano-hydroxyapatite (n-HA) into poly (lactic acid-glycolic acid) (PLAGA) microsphere based scaffolds in our previous studies. Herein, we aimed to evaluate the bioactivity of PLAGA/n-HA composite scaffolds, with a focus on studying the mineralization of the scaffolds in vitro. The capability of inducing apatite formation in vitro was largely enhanced in the composite scaffolds compared to plain PLAGA scaffolds. More importantly, PLAGA/n-HA composite scaffolds have been shown to improve rabbit mesenchymal stem cells (RMSCs) proliferation, differentiation, and mineralization as compared to control PLAGA scaffolds. Taken together, introduction of n-HA appears to be an efficient approach to improve the bioactivity of PLAGA scaffolds for bone tissue engineering.

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“…Both micro-/nanoporous topography of MAO and the functional groups of PDA and heparin are previously shown to direct protein adsorption and cell adhesion and further promote osteoblast spreading, migration and proliferation [5]. Further, the release of Ca 2+ from the scaffold into the extracellular environment is beneficial to promoting the osteogenic differentiation of hMSCs on the scaffold [47]. And the higher ALP activity, an early phase indicator of the osteoblastic differentiation of hMSCs, exhibited by the treated group, demonstrates this point.…”

Section: Discussionmentioning

confidence: 99%

Polydopamine-assisted functionalization of heparin and vancomycin onto microarc-oxidized 3D printed porous Ti6Al4V for improved hemocompatibility, osteogenic and anti-infection potencies

et al. 2018

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A calcium-induced signaling cascade leading to osteogenic differentiation of human bone marrow-derived mesenchymal stromal cells

Cited by 396 publications

References 63 publications

Fe³⁺/⁻ dual doped nano hydroxyapatite: A novel material for biomedical applications

Fe³⁺/⁻ dual doped nano hydroxyapatite: A novel material for biomedical applications

Evaluation of PLAGA/n-HA Composite Scaffold Bioactivity in vitro

Polydopamine-assisted functionalization of heparin and vancomycin onto microarc-oxidized 3D printed porous Ti6Al4V for improved hemocompatibility, osteogenic and anti-infection potencies

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A calcium-induced signaling cascade leading to osteogenic differentiation of human bone marrow-derived mesenchymal stromal cells

Cited by 396 publications

References 63 publications

Fe3+/− dual doped nano hydroxyapatite: A novel material for biomedical applications

Fe3+/− dual doped nano hydroxyapatite: A novel material for biomedical applications

Evaluation of PLAGA/n-HA Composite Scaffold Bioactivity in vitro

Polydopamine-assisted functionalization of heparin and vancomycin onto microarc-oxidized 3D printed porous Ti6Al4V for improved hemocompatibility, osteogenic and anti-infection potencies

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Product

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Fe³⁺/⁻ dual doped nano hydroxyapatite: A novel material for biomedical applications

Fe³⁺/⁻ dual doped nano hydroxyapatite: A novel material for biomedical applications