A catalytic triplex DNAzyme for porphyrin metalation

Zheng, Xiong; Yang, Mujing; Yang, Tong; Chang, Yun; Peng, Shuzhen; Xu, Qiuda; Wang, Dandan; Zhou, Xiao‐Shun; Shao, Yong

doi:10.1039/d1cc01955d

Cited by 5 publications

(3 citation statements)

References 47 publications

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“…Later on, artificial DNA molecules were also identified with enzymatic activities (DNAzyme) that allow them to mediate RNA/DNA cleavage and ligation, porphyrin metalation, redox reactions as peroxidases do, etc. [ 70 ]. Currently, a new generation of NAs known as aptazymes is being used for biosensing, which integrates aptamer and DNAzyme technologies to specifically identify a target and catalyze biochemical reactions [ 71 ].…”

Section: Mxene In Na Biosensorsmentioning

confidence: 99%

MXene-Based Nucleic Acid Biosensors for Agricultural and Food Systems

Wang

Gunasekaran

2022

Biosensors

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MXene is a two-dimensional (2D) nanomaterial that exhibits several superior properties suitable for fabricating biosensors. Likewise, the nucleic acid (NA) in oligomerization forms possesses highly specific biorecognition ability and other features amenable to biosensing. Hence the combined use of MXene and NA is becoming increasingly common in biosensor design and development. In this review, MXene- and NA-based biosensors are discussed in terms of their sensing mechanisms and fabrication details. MXenes are introduced from their definition and synthesis process to their characterization followed by their use in NA-mediated biosensor fabrication. The emphasis is placed on the detection of various targets relevant to agricultural and food systems, including microbial pathogens, chemical toxicants, heavy metals, organic pollutants, etc. Finally, current challenges and future perspectives are presented with an eye toward the development of advanced biosensors with improved detection performance.

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Section: Mxene In Na Biosensorsmentioning

confidence: 99%

MXene-Based Nucleic Acid Biosensors for Agricultural and Food Systems

Wang

Gunasekaran

2022

Biosensors

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“…1,2 It has been established that triplex DNAs have wide biological activities including regulation of gene expression, 3−6 initiation of DNA damage and apoptosis, 7−9 release of drugs, 10 and even participation of cancer progression. 11 In addition, with their structural uniqueness, triplex DNAs have been widely explored in molecular switches, 12−17 nanodevices, 18−20 DNA crystals, 21 catalysis, 22 and directing chemical reactions. 23 Practically, triplex DNAs have also been employed as sensor elements for the recognition and analysis of nucleic acids, 24−30 proteins, 17,31,32 toxic metal ions, 33,34 pH, 35 and bioactive small molecules.…”

mentioning

confidence: 99%

“…DNA triplexes are formed by the sequence-selective recognition of triplex-forming oligonucleotides (TFOs) toward DNA duplexes at the major groove. , It has been established that triplex DNAs have wide biological activities including regulation of gene expression, − initiation of DNA damage and apoptosis, − release of drugs, and even participation of cancer progression . In addition, with their structural uniqueness, triplex DNAs have been widely explored in molecular switches, − nanodevices, − DNA crystals, catalysis, and directing chemical reactions . Practically, triplex DNAs have also been employed as sensor elements for the recognition and analysis of nucleic acids, − proteins, ,, toxic metal ions, , pH, and bioactive small molecules. ,− Furthermore, versatile chemical modifications with DNAs diversify the triplex structures with promise even in cell manipulation.…”

mentioning

confidence: 99%

Polarity-Specific and Pyrimidine-over-Purine Adaptive Triplex DNA Recognition by a Near-Infrared Fluorogenic Molecular Rotor

Zeng,

Xu,

Lai

et al. 2023

Anal. Chem.

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Triplex DNA structures have displayed a wide range of applications including nanosensing, molecule switching, and drug delivering. Therefore, it is of great importance to effectively recognize triplex DNA structures by a simple and highly selective manner. Herein, we found that a near-infrared fluorogenic probe of NIAD-4 with a molecular rotor (MR) merit can selectively recognize triplex DNA structures over G-quadruplex, i-motif, and duplex structures (Tri-over-QID selectivity), which is competent over the widely used MR probe of thioflavin T (ThT). Furthermore, NIAD-4 exhibits as well a high selectivity toward the ′pyrimidine-type′ triplex structures (Y:R-Y type) with respect to the ′purine-type′ triplex structures (R:R-Y type) (a Y-over-R selectivity). Interestingly, NIAD-4 recognizes the Y:R-Y triplex structures by a polarity-dependent manner. The 3′ end triplet is the preferential binding field of NIAD-4 with respect to the 5′ end one (a 3′-over-5′ selectivity) as the 3′ end triplet is more stable than the 5′ end one in the Hoogsteen hydrogen bond. It is expected that the adaptive stacking interaction between NIAD-4 and the 3′ end triplet favors the Tri-over-QID, Y-over-R, and 3′-over-5′ selectivities since this MR probe has three rotating shafts matching well with the triplet in topology. Such a high selectivity of NIAD-4 opens a new route in designing sensors with DNA structures switching between triplex, i-motif, and G-quadruplex structures.

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