A CFA-Induced Model of Inflammatory Skin Disease in Miniature Swine

Navarro-Alvarez, Nalú; Gonçalves, Beatriz M; Andrews, Alec R.; Sachs, David H.; Huang, Christene A.

doi:10.1155/2018/6916920

Cited by 9 publications

(7 citation statements)

References 22 publications

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“…We finally carried out intraplantar injection of CFA to determine whether γδT cells contribute to chronic inflammatory pain, an example of granulomatous inflammation that does not resolve ( 44 ). Similar to the formalin and incisional wound models, no significant effects were observed in male ( n = 9–12) or female ( n = 6–9) littermates when assessed for mechanical ( P ≥ 0.226, two-way RM-ANOVA) and thermal ( P ≥ 0.857, two-way RM-ANOVA) hypersensitivity over 7 days ( Figure 2 ).…”

Section: Resultsmentioning

confidence: 99%

γδ T Cells Modulate Myeloid Cell Recruitment but Not Pain During Peripheral Inflammation

et al. 2019

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Circulating immune cells, which are recruited to the site of injury/disease, secrete various inflammatory mediators that are critical to nociception and pain. The role of tissue-resident immune cells, however, remains poorly characterized. One of the first cells to be activated in peripheral tissues following injury are γδT cells, which serve important roles in infection, disease, and wound healing. Using a mouse line lacking these cells, we sought to identify their contribution to inflammatory pain. Three distinct models of peripheral inflammatory pain were used: intraplantar injection of formalin (spontaneous inflammatory pain), incisional wound (acute inflammatory pain), and intraplantar injection of complete Freund's adjuvant (chronic inflammatory pain). Our results show that absence of γδT cells does not alter baseline sensitivity, nor does it result in changes to mechanical or thermal hypersensitivity after tissue injury. Myeloid cell recruitment did show differential changes between models of acute and chronic inflammatory pain. These results were consistent in both male and female mice, suggesting that there are no sex differences in these outcomes. This comprehensive characterization suggests that γδT cells do not contribute to basal sensitivity or the development and maintenance of inflammatory pain.

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Section: Resultsmentioning

confidence: 99%

γδ T Cells Modulate Myeloid Cell Recruitment but Not Pain During Peripheral Inflammation

et al. 2019

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“…CFA consist of mineral oil, containing a suspension of whole or pulverized heat-killed mycobacteria and its adjuvant activity results from the sustained release of antigens from the oily deposit and stimulation of a local innate immune response causing a delayed hyper-sensitivity reaction with an intense inflammatory reaction at the side of injection. 13 A previous study showed that sub-plantar injection of CFA resulted in a significant elevation in IL-1B as a proinflammatory cytokine. 20 The paw edema thickness of treatment group (Kp1, Kp2, Kp3) when compared to normal group (Kn) still have big difference in value (figure 1) because subcutaneous injection of CFA induced inflammation is still in the acute phase (0-10 days) is caused by various mediators such as histamine, serotonin, kinins and prostaglandins, released by leukocytes that migrate to the affected region and provoked a vasculo-exsudatif phenomena responsible edema.…”

Section: Figure 1 Hind Paw Edema Of Wistar Rats After 24 Hours Cfa Imentioning

confidence: 98%

“…Role of CFA is concluded that adjuvant activity from sustain release of antigens from the oily deposit and stimulation of local innate immune response causing delayed hypersensitivity reaction that can react with intense inflammatory reaction and hyperalgesia at the site of injection. 13 Therefore, this study aimed to prove the potency of bay leaves extract to prevent the inflammation, which is indicated by decreasing the paw edema thickness in rats induced with CFA.…”

Section: Introductionmentioning

confidence: 99%

The potency of bay leaves extract (Syzygiumpolyanthum) as anti-inflammation in Rattus norvegicus induced with Complete Freund’s Adjuvant

2019

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The using of herbal plants in Indonesia as medicine based on traditional knowledge. One of the herbal plants that believed has medication effect is bay leaves (Syzygiumpolyanthum). Bay leaves contain a flavonoid that can prevent inflammation. The study aims to prove the potency of bay leaves extract to prevent the inflammation, which is indicated by decreasing the paw edema thickness in rat induced with Complete Freund's Adjuvant (CFA). This study used 25 animal models of male rat (Rattus norvegicus) strain Wistar that divided into five groups, one group for a normal group (no treatment), one group for positive control which was injected with CFA sub plantar and given treatment with Na CMC and three test groups which is got three different doses of bay leaves extract peroral and the doses were 100 mg/kg BW, 300 mg/kg BW, 400 mg/kg BW, and injected with CFA into the sub plantar. Bay leaves extract was given one hour per oral before injected with CFA. The measurement of paw edema was done at 24 hours after CFA injection. The result of this study showed that bay leaves extract gave the anti-inflammation effect to the rats which were injected with CFA. It is concluded that bay leaves extract containing flavonoids plays a role in inhibit cytokines proinflammatory synthesis.

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“…or S.C. injection. Complete Freund’s adjuvant (CFA) is a mineral oil containing heat-killed mycobacteria that are incapable of causing disease in animals ( Navarro-Alvarez et al, 2018 ). Intraplantar injection of CFA evokes an inflammatory pain response at the site of injection, resulting in paw swelling and tactile allodynia in behavioral testing which is reduced following GlyT inhibitor administration ( Ferreira et al, 2001 ; Kassuya et al, 2003 ; Morita et al, 2008 ; Chang et al, 2010 ; Abboud et al, 2021 ).…”

Section: Pain Modelsmentioning

confidence: 99%

Glycinergic Modulation of Pain in Behavioral Animal Models

Peiser-Oliver

Evans²,

Adams³

et al. 2022

Front. Pharmacol.

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Animal models of human pain conditions allow for detailed interrogation of known and hypothesized mechanisms of pain physiology in awake, behaving organisms. The importance of the glycinergic system for pain modulation is well known; however, manipulation of this system to treat and alleviate pain has not yet reached the sophistication required for the clinic. Here, we review the current literature on what animal behavioral studies have allowed us to elucidate about glycinergic pain modulation, and the progress toward clinical treatments so far. First, we outline the animal pain models that have been used, such as nerve injury models for neuropathic pain, chemogenic pain models for acute and inflammatory pain, and other models that mimic painful human pathologies such as diabetic neuropathy. We then discuss the genetic approaches to animal models that have identified the crucial glycinergic machinery involved in neuropathic and inflammatory pain. Specifically, two glycine receptor (GlyR) subtypes, GlyRα1(β) and GlyRα3(β), and the two glycine transporters (GlyT), GlyT1 and GlyT2. Finally, we review the different pharmacological approaches to manipulating the glycinergic system for pain management in animal models, such as partial vs. full agonism, reversibility, and multi-target approaches. We discuss the benefits and pitfalls of using animal models in drug development broadly, as well as the progress of glycinergic treatments from preclinical to clinical trials.

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A CFA-Induced Model of Inflammatory Skin Disease in Miniature Swine

Cited by 9 publications

References 22 publications

γδ T Cells Modulate Myeloid Cell Recruitment but Not Pain During Peripheral Inflammation

γδ T Cells Modulate Myeloid Cell Recruitment but Not Pain During Peripheral Inflammation

The potency of bay leaves extract (Syzygiumpolyanthum) as anti-inflammation in Rattus norvegicus induced with Complete Freund’s Adjuvant

Glycinergic Modulation of Pain in Behavioral Animal Models

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