A chimeric protein of abrin and Abrus precatorius agglutinin that neutralizes abrin mediated lethality in mice

Tiwari, Vivek; Bagaria, Shradha; Karande, Anjali A.

doi:10.1016/j.toxicon.2017.01.008

Cited by 8 publications

(2 citation statements)

References 42 publications

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“…In mice, generation of protective antibodies can be induced with a sub-lethal dose of abrin (10,11). Recombinant abrin A chain (12)(13)(14)(15) or the weaker abrin B chain (16)(17)(18)(19), can also induce anti-abrin antibody production. Up to now, only two neutralizing antibodies against Abrin poisoning have been reported, D6F10 and A7C4, but neither has entered clinical trials or been used directly in humans.…”

Section: Introductionmentioning

confidence: 99%

A Novel Humanized Anti-Abrin A Chain Antibody Inhibits Abrin Toxicity In Vitro and In Vivo

Peng

Shi

et al. 2022

Front. Immunol.

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Abrin, a type-II ribosome inactivating protein from the seed of Abrus precatorius, is classified as a Category B bioterrorism warfare agent. Due to its high toxicity, ingestion by animals or humans will lead to death from multiple organ failure. Currently, no effective agents have been reported to treat abrin poisoning. In this study, a novel anti-abrin neutralizing antibody (S008) was humanized using computer-aided design, which possessed lower immunogenicity. Similar to the parent antibody, a mouse anti-abrin monoclonal antibody, S008 possessed high affinity and showed a protective effect against abrin both in vitro and in vivo, and protected mice that S008 was administered 6 hours after abrin. S008 was found that it did not inhibit entry of abrin into cells, suggesting an intracellular blockade capacity against the toxin. In conclusion, this work demonstrates that S008 is a high affinity anti-abrin antibody with both a neutralizing and protective effect and may be an excellent candidate for clinical treatment of abrin poisoning.

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Section: Introductionmentioning

confidence: 99%

A Novel Humanized Anti-Abrin A Chain Antibody Inhibits Abrin Toxicity In Vitro and In Vivo

Peng

Shi

et al. 2022

Front. Immunol.

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“…Owing of its stability, extreme toxicity and ease of purification abrin is classified as select agent by CDC. LD 50 dose is only 0.04 µg/kg body weight of mice [94]. Estimated fatal dose for humans is 0.1–1 µg kg −1 body weight.…”

Section: Introductionmentioning

confidence: 99%

Biological Toxins as the Potential Tools for Bioterrorism

Janik

Ceremuga

Saluk‐Bijak

et al. 2019

IJMS

115

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Biological toxins are a heterogeneous group produced by living organisms. One dictionary defines them as “Chemicals produced by living organisms that have toxic properties for another organism”. Toxins are very attractive to terrorists for use in acts of bioterrorism. The first reason is that many biological toxins can be obtained very easily. Simple bacterial culturing systems and extraction equipment dedicated to plant toxins are cheap and easily available, and can even be constructed at home. Many toxins affect the nervous systems of mammals by interfering with the transmission of nerve impulses, which gives them their high potential in bioterrorist attacks. Others are responsible for blockage of main cellular metabolism, causing cellular death. Moreover, most toxins act very quickly and are lethal in low doses (LD50 < 25 mg/kg), which are very often lower than chemical warfare agents. For these reasons we decided to prepare this review paper which main aim is to present the high potential of biological toxins as factors of bioterrorism describing the general characteristics, mechanisms of action and treatment of most potent biological toxins. In this paper we focused on six most danger toxins: botulinum toxin, staphylococcal enterotoxins, Clostridium perfringens toxins, ricin, abrin and T-2 toxin. We hope that this paper will help in understanding the problem of availability and potential of biological toxins.

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Phytoconstituents and ethnopharmacological activities of Abrus precatorius L. (Fabaceae): a review

Kaula

Mishra

Prakash

et al. 2022

Vegetos

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A chimeric protein of abrin and Abrus precatorius agglutinin that neutralizes abrin mediated lethality in mice

Cited by 8 publications

References 42 publications

A Novel Humanized Anti-Abrin A Chain Antibody Inhibits Abrin Toxicity In Vitro and In Vivo

A Novel Humanized Anti-Abrin A Chain Antibody Inhibits Abrin Toxicity In Vitro and In Vivo

Biological Toxins as the Potential Tools for Bioterrorism

Phytoconstituents and ethnopharmacological activities of Abrus precatorius L. (Fabaceae): a review

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