A Chitin Nanofiber Ink for Airbrushing, Replica Molding, and Microcontact Printing of Self‐assembled Macro‐, Micro‐, and Nanostructures

Zhong, Chao; Kapetanovic, Adnan; Deng, Yingxin; Rolandi, Marco

doi:10.1002/adma.201102639

Cited by 79 publications

(61 citation statements)

References 33 publications

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“…The patterned elastomer can then be used to create topographies via replica molding, micromolding in capillaries, microtransfer molding, and microfluidics [57-59]. Numerous studies have used replica molding to develop 3D topographies through casting or embossing polymeric replicas on the original master or elastomeric molds [60]. Both rigid and elastomeric molds may be used to replicate the polymeric materials [61].…”

Section: Fabrication Techniquesmentioning

confidence: 99%

Engineering microscale topographies to control the cell–substrate interface

et al. 2012

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Cells in their in vivo microenvironment constantly encounter and respond to a multitude of signals. While the role of biochemical signals has long been appreciated, the importance of biophysical signals has only recently been investigated. Biophysical cues are presented in different forms including topography and mechanical stiffness imparted by the extracellular matrix and adjoining cells. Microfabrication technologies have allowed for the generation of biomaterials with microscale topographies to study the effect of biophysical cues on cellular function at the cell–substrate interface. Topographies of different geometries and with varying microscale dimensions have been used to better understand cell adhesion, migration, and differentiation at the cellular and sub-cellular scales. Furthermore, quantification of cell-generated forces has been illustrated with micropillar topographies to shed light on the process of mechanotransduction. In this review, we highlight recent advances made in these areas and how they have been utilized for neural, cardiac, and musculoskeletal tissue engineering application.

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Section: Fabrication Techniquesmentioning

confidence: 99%

Engineering microscale topographies to control the cell–substrate interface

et al. 2012

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“…20 We have previously developed the self-assembly of 3 nm chitin nanofibers from solution 17, 21, 22 and demonstrated simple solution co-assembly of chitin nanofiber-silk biocomposites. 23 This solution process is amenable to facile soft-lithography strategies to create microstructures 24 for tissue engineering 17, 25 and biomedical devices. 19 …”

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confidence: 99%

Ultrastrong and flexible hybrid hydrogels based on solution self-assembly of chitin nanofibers in gelatin methacryloyl (GelMA)

et al. 2016

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“…Nebulization technologies have been employed in the development of many innovative materials, including the fabrication of nanofiber coatings [24,25], light-emitting electrochemical cell coatings [26], coating and patterning of films with proteins or other molecules [27,28], and cell patterning and implantation [29–31]. The present study expands on previous nebulization technologies by developing a nebulization technique that produces smooth, isotropic topographical regions in aligned PLLA fiber scaffolds.…”

Section: Introductionmentioning

confidence: 98%

Nebulized solvent ablation of aligned PLLA fibers for the study of neurite response to anisotropic-to-isotropic fiber/film transition (AFFT) boundaries in astrocyte–neuron co-cultures

et al. 2015

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Developing robust in vitro models of in vivo environments has the potential to reduce costs and bring new therapies from the bench top to the clinic more efficiently. This study aimed to develop a biomaterial platform capable of modeling isotropic-to-anisotropic cellular transitions observed in vivo, specifically focusing on changes in cellular organization following spinal cord injury. In order to accomplish this goal, nebulized solvent patterning of aligned, electrospun poly-l-lactic acid (PLLA) fiber substrates was developed. This method produced a clear topographic transitional boundary between aligned PLLA fibers and an isotropic PLLA film region. Astrocytes were then seeded on these scaffolds, and a shift between oriented and non-oriented astrocytes was created at the anisotropic-to-isotropic fiber/film transition (AFFT) boundary. Orientation of chondroitin sulfate proteoglycans (CSPGs) and fibronectin produced by these astrocytes was analyzed, and it was found that astrocytes growing on the aligned fibers produced aligned arrays of CSPGs and fibronectin, while astrocytes growing on the isotropic film region produced randomly-oriented CSPG and fibronectin arrays. Neurite extension from rat dissociated dorsal root ganglia (DRG) was studied on astrocytes cultured on anisotropic, aligned fibers, isotropic films, or from fibers to films. It was found that neurite extension was oriented and longer on PLLA fibers compared to PLLA films. When dissociated DRG were cultured on the astrocytes near the AFFT boundary, neurites showed directed orientation that was lost upon growth into the isotropic film region. The AFFT boundary also restricted neurite extension, limiting the extension of neurites once they grew from the fibers and into the isotropic film region. This study reveals the importance of anisotropic-to-isotropic transitions restricting neurite outgrowth by itself. Furthermore, we present this scaffold as an alternative culture system to analyze neurite response to cellular boundaries created following spinal cord injury and suggest its usefulness to study cellular responses to any aligned-to-unorganized cellular boundaries seen in vivo.

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A Chitin Nanofiber Ink for Airbrushing, Replica Molding, and Microcontact Printing of Self‐assembled Macro‐, Micro‐, and Nanostructures

Cited by 79 publications

References 33 publications

Engineering microscale topographies to control the cell–substrate interface

Engineering microscale topographies to control the cell–substrate interface

Ultrastrong and flexible hybrid hydrogels based on solution self-assembly of chitin nanofibers in gelatin methacryloyl (GelMA)

Nebulized solvent ablation of aligned PLLA fibers for the study of neurite response to anisotropic-to-isotropic fiber/film transition (AFFT) boundaries in astrocyte–neuron co-cultures

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