A Chronic Oral Toxicity Study of Marine Collagen Peptides Preparation from Chum Salmon (Oncorhynchus keta) Skin Using Sprague-Dawley Rat

Jiang, Liang; Pei, Xinrong; Zhang, Zhaofeng; Wang, Nan; Wang, Junbo; Li, Yong

doi:10.3390/md10010020

Cited by 17 publications

(15 citation statements)

References 23 publications

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“…Despite the fact that there are no previous reports available on the hepato-toxicity induced by oral inoculation of food-derived collagen [20], our records have demonstrated histopathological alterations in liver after 30 days of collagen feeding. Similarly, Woo et al, have been mentioned that the collagen-fed mice had a lower level of hepatic Triglyceride; which was consistent with liver histological results.…”

Section: Discussioncontrasting

confidence: 81%

A Chronic Toxicity Study of Oral Administration of Collagen-?® Supplement using Pregnant Rabbits

Kudayer¹,

Alwan²,

Sawad³

2020

IJFMT

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Since collagen derived products are highly used in food supplements due to its bioactive properties by influencing cellular and tissue health, flexibility with high effect of repairing and adaptation. It is necessary to carry out safety requirement of an oral chronic toxicity assessment. This study investigated the adverse effect of long term oral administration of collagen-® in pregnant rabbits. We have conducted a thirty days randomized controlled trial in pregnant rabbits. Rabbits were randomized into two groups; Group 1 (control group) were administrated with 1 ml of normal saline PO, SID while Group 2 (collagen-α group) were administered with 1 ml of collagen-α ® PO, SID for one month. Bodyweight gain, viscera weight and histopathological evaluation of the individual rabbit were recorded. The results revealed that the body weight gain and visceral tissues weight in the collagen-α group were significantly lower than the control group. Moreover, various histopathological changes were recorded in heart, lungs, liver and kidneys of the collagen-α group when compared with the non-significant changes that noticed in the control group. Under the conditions of the current study, a high risk of chronic toxic effect was observed in pregnant rabbits inoculated with 1 ml of oral dose of collagen alpha supplement. This result suggests that the adverse effect of collagen derived supplements is overbalancing the benefits.

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Section: Discussioncontrasting

confidence: 81%

A Chronic Toxicity Study of Oral Administration of Collagen-?® Supplement using Pregnant Rabbits

Kudayer¹,

Alwan²,

Sawad³

2020

IJFMT

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“…MCPs are low-molecular-weight peptides extracted from collagen tissues of marine fish, including skin, bone and scales, which account for 30% of marine fish processing waste ( 36 ). Previous investigations have confirmed that these protein fragments are safe in animals and humans ( 24 ). We previously tested the therapeutic effects of MCPs in diabetic animals ( 13 ) and patients ( 10 , 12 , 21 ).…”

Section: Discussionmentioning

confidence: 71%

“…At 1–2 weeks later, fasting blood glucose (FBG) of rats were detected by a blood glucose meter, and 45 rats with FBG levels ≥11.1 mmol/l were considered as T2DM and were used for the experiment. A total of 40 of these diabetic rats were then assigned randomly to 4 groups treated with vehicle (group D), or 2.25 (low dose, group L), 4.5 (medium dose, group M) or 9.0 (high dose, group H) g/kg BW/day MCPs (n=10/group) ( 24 ). The MCPs and vehicle (distilled water; total volume was 1 ml/application) were administered intragastrically every morning after the T2DM model had been established for 4 weeks.…”

Section: Methodsmentioning

confidence: 99%

Marine collagen peptides reduce endothelial cell injury in diabetic rats by inhibiting apoptosis and the expression of coupling factor 6 and microparticles

Zhu

Zhang

Liu

et al. 2017

Molecular Medicine Reports

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The present study aimed to elucidate the role of marine collagen peptides (MCPs) in protection of carotid artery vascular endothelial cells (CAVECs) in type 2 diabetes mellitus (T2DM), and the mechanism underlying this process. In an in vivo experiment, diabetic Wistar rats were divided randomly into four groups (n=10/group): Diabetes control, and three diabetes groups administered low, medium and high doses of MCPs (2.25, 4.5 and 9.0 g/kg body weight/day, respectively). Another 10 healthy rats served as the control. In an in vitro experiment, human umbilical-vein endothelial cells (HUVECs) were incubated in normal and high concentrations of glucose with or without MCPs (3.0, 15.0 and 30.0 mg/ml, respectively) for 24, 48 or 72 h. Blood vessel/endothelial construction, inflammatory exudation and associated molecular biomarkers in CAVECs were detected and analyzed. The results of the present study demonstrated that in rats, MCP treatment for 4 weeks significantly lowered blood glucose and attenuated endothelial thinning and inflammatory exudation in carotid-artery vascular endothelial cells. In vitro, the high-glucose intervention significantly increased cell apoptosis in HUVECs, and medium and high doses of MCPs (4.5 and 9.0 g/kg body weight/day, respectively) partially ameliorated this high glucose-mediated apoptosis and decreased levels of apoptosis biomarkers. In conclusion, a moderate oral MCP dose (≥4.5 g/kg body weight/day) may be a novel therapeutic tool to protect against early cardiovascular complications associated with T2DM by inhibiting apoptosis and reducing the expression of coupling factor 6 and microparticles.

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“…Experimental design and T2DM rat model T2DM was induced according to methods described in our previous study (Srinivasan et al 2005). After 2 weeks of HCHFD feeding, freshly prepared streptozotocin [STZ, 30 mg/kg body weight (BW)] in citrate buffer (0.1 M, pH 4.5) was administered intraperitoneally three times a week (Liang et al 2012). At approximately 2 weeks following STZ, fasting blood glucose (FBG) levels were measured using a blood glucose meter (Oxidase methods, OneTouch Ultra; Lifescan, Wayne, PA, USA).…”

Section: Rat Model Of T2dmmentioning

confidence: 99%

“…Of the 50 rats, 45 rats were considered to have T2DM with an FBG level C11.1 mmol/l. Forty of these diabetic rats were then randomly assigned to four groups: vehicle (group D, n = 10), low dose MCPs (2.25 g/kg/day; group L, n = 10), medium dose MCPs (4.5 g/kg/day, group M, n = 10), or high dose MCPs (9.0 g/kg/day, group H, n = 10) (Liang et al 2012).…”

Section: Rat Model Of T2dmmentioning

confidence: 99%

Effects of marine collagen peptides on glucose metabolism and insulin resistance in type 2 diabetic rats

Zhu

Zhang

et al. 2017

J Food Sci Technol

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The present study was conducted to investigate the effects of marine collagen peptides (MCPs) on glucose metabolism and insulin resistance using a rat model of type 2 diabetes mellitus (T2DM). Forty T2DM obese Wistar rats were randomly assigned to receive varying doses of MCPs or a vehicle control for 4 weeks. Blood glucose and insulin levels, as well as oxidative stress and inflammation were measured. The expression of glucose transporter type 4 (GLUT4) in skeletal muscles and peroxisome proliferator-activated receptor-α (PPAR-α) in livers of T2DM rats was also measured. It was found that in the group of 9.0 g/kg/day MCPs significantly improved glucose, insulin, and homeostatic model assessment-insulin resistance, and increased the insulin sensitivity index (ISI). In addition, the groups of 4.5 and 2.25 g/kg/day MCPs significantly improved liver steatosis. It was also found that MCPs decreased expression of oxidative stress biomarkers and inflammatory cytokines and adipocytokines in T2DM rats. In conclusion, medium and high doses of MCPs (≥4.5 g/kg/day) improved glucose metabolism and insulin sensitivity in T2DM rats. These beneficial effects of MCPs may be mediated by decreasing oxidative stress and inflammation and by up-regulating GLUT4, and PPAR-α activity.

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A Chronic Oral Toxicity Study of Marine Collagen Peptides Preparation from Chum Salmon (Oncorhynchus keta) Skin Using Sprague-Dawley Rat

Cited by 17 publications

References 23 publications

A Chronic Toxicity Study of Oral Administration of Collagen-?® Supplement using Pregnant Rabbits

A Chronic Toxicity Study of Oral Administration of Collagen-?® Supplement using Pregnant Rabbits

Marine collagen peptides reduce endothelial cell injury in diabetic rats by inhibiting apoptosis and the expression of coupling factor 6 and microparticles

Effects of marine collagen peptides on glucose metabolism and insulin resistance in type 2 diabetic rats

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