A collection of<i>Serratia marcescens</i>differing in their insect pathogenicity towards<i>Manduca sexta</i>larvae

Jensen, Ellen C.; Amburn, D. Sue Katz; Schlegel, Aimee Hultine; Nickerson, Kenneth W.

doi:10.1101/2020.07.29.226613

Cited by 4 publications

(3 citation statements)

References 41 publications

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“…2B , 3-PPA (50.0 μg/mL) treatment resulted in a 20% inhibition of protease compared with the DMSO group. Lipase is involved in degrading phospholipid bilayers and mediating cellular signaling pathways in the host ( 18 ). As shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

3-Phenylpropan-1-Amine Enhanced Susceptibility of Serratia marcescens to Ofloxacin by Occluding Quorum Sensing

et al. 2022

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Section: Resultsmentioning

confidence: 99%

3-Phenylpropan-1-Amine Enhanced Susceptibility of Serratia marcescens to Ofloxacin by Occluding Quorum Sensing

et al. 2022

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“…The following bacteria were injected in at least four experimental blocks: Providencia rettgeri strain Dmel, Serratia marcescens strain 2698B, Enterococcus faecalis strain Dmel , Lactococcus lactis strain Dmel. All bacteria were originally isolated as natural infections of D. melanogaster (36,73) except S. marcescens 2698B, which is a clinical isolate (74). Cultures for P. rettgeri and S. marcescens were started from a frozen glycerol stock then cultured overnight in liquid lysogeny broth (LB) at 37 °C on a shaker, then subcultured the next morning in fresh LB to achieve growth phase.…”

Section: Methodsmentioning

confidence: 99%

High sugar diets can increase susceptibility to bacterial infection inDrosophila melanogaster

Darby,

Okoro,

Aredas

et al. 2023

Preprint

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Overnutrition with dietary sugar can worsen infection outcomes in diverse organisms including insects and humans, generally through unknown mechanisms. In the present study, we show that adultDrosophila melanogasterfed high-sugar diets became more susceptible to infection by the Gram-negative bacteriaProvidencia rettgeriandSerratia marcescens,although diet had no significant effect on infection by Gram-positive bacteriaEnterococcus faecalisorLactococcus lactis.We found thatP. rettgeriandS. marcescensproliferate more rapidly inD. melanogasterfed a high-sugar diet, resulting in increased probability of host death.D. melanogasterbecome hyperglycemic on the high-sugar diet, and we find evidence that the extra carbon availability may promoteS. marcescensgrowth within the host. However, we found no evidence that increased carbon availability directly supports greaterP. rettgerigrowth.D. melanogasteron both diets fully induce transcription of antimicrobial peptide (AMP) genes in response to infection, butD. melanogasterprovided with high-sugar diets show reduced production of AMP protein. Thus, overnutrition with dietary sugar may impair host immunity at the level of AMP translation. Our results demonstrate that dietary sugar can shape infection dynamics by impacting both host and pathogen, depending on the nutritional requirements of the pathogen and by altering the physiological capacity of the host to sustain an immune response.Author SummaryDiet has critical impact on the quality of immune defense, and high-sugar diets increase susceptibility to bacterial infection in many animals. Yet it is unknown which aspects of host and pathogen physiology are impacted by diet to influence infection dynamics. Here we show that high-sugar diets increase susceptibility to some, but not all, bacterial infections inDrosophila. We find that feeding on high sugar diet impairs the host immune response by reducing the level of antimicrobial peptides produced. The expression of genes encoding these peptides is not affected, so we infer that protein translation is impaired. We further show that flies on high-sugar diets are hyperglycemic, and that some pathogens may use the excess sugar in the host to promote growth during the infection. Thus, our study demonstrates that dietary impacts on infection outcome arise through physiological effects on both the host and pathogen.

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“…from mountain soil, and Serratia species from coastal water, have been documented previously [10]. However, Serratia species have been reported to produce proteases, DNases, and lipases [11].…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of a cold‐active, detergent‐stable protease from Serratia sp. TGS1

et al. 2023

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Psychrophiles are cold‐adapted microorganisms living in cold regions and are known to generate cold‐active enzymes such as proteases, lipases, and peptidases. These types of enzymes are a major part of the market of the food and textile sector. This study aimed to isolate and characterize the cold‐active and detergent‐stable, extracellular protease from psychotrophic bacteria Serratia sp. TGS1 (OQ654005). Protease was purified by gel permeation chromatography using Sephadex G‐75. The specific activity of the purified protease was 250 U/mg at 15°C, with a purification fold of 5.68 and a percentage yield of 60%. The cold active protease was stable within a temperature range of 5–30°C and a pH range of 6–10. Ca+2 and Mg+2 enhanced its activity while chelators like ethylenediaminetetraacetic acid inhibited cold active protease, showing it as metalloprotease in nature. The enzyme was sensitive to Cu+2, Zn+2, and Hg+2, and the proteolytic activity decreased upon treatment with heavy metals. The molecular weight of the protease was estimated to be 47 kDa using sodium dodecyl sulfate (SDS)‐polyacrylamide gel electrophoresis. Proteins within a specific range of molecular weight possess desirable properties for industrial enzyme use. By working on a specific range, the researchers intended to examine an enzyme to examine its specific characteristics. The purified protease showed high stability to detergents like SDS, Tween 20, Tween 60, and Triton X. The maximum velocity Vmax and Km values were 59.90 mg/min/mL and 1.53 mg/mL, respectively. The obtained protease exhibited an interesting activity at a broad range of pH (6–10) and stability at low temperatures (5–30°C) and detergents. Such enzymatic features of versatile and potent cold‐active enzymes enhance their industrial applications to meet food, dairy, and laundry requirements.

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A collection ofSerratia marcescensdiffering in their insect pathogenicity towardsManduca sextalarvae

Cited by 4 publications

References 41 publications

3-Phenylpropan-1-Amine Enhanced Susceptibility of Serratia marcescens to Ofloxacin by Occluding Quorum Sensing

3-Phenylpropan-1-Amine Enhanced Susceptibility of Serratia marcescens to Ofloxacin by Occluding Quorum Sensing

High sugar diets can increase susceptibility to bacterial infection inDrosophila melanogaster

Isolation and characterization of a cold‐active, detergent‐stable protease from Serratia sp. TGS1

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