A colorimetric and fluorometric microplate assay for the detection of microcystin-LR in drinking water without preconcentration

Towards the protein phosphatase-based biosensor for microcystin detection

“…Enzyme-linked immunosorbent assays (ELISA) using monoclonal or polyclonal antibodies show high specificity but do not provide information about the toxicity of microcystins and usually present cross-reactivity problems (Brooks and Codd, 1988;Chu et al, 1990;Nagata et al, 1995). On the contrary, the enzymatic method, based on the PP inhibition, informs about the toxicology and is highly sensitive (MacKintosh et al, 1990;An and Carmichael, 1994;Ward et al, 1997;Rivasseau et al, 1999;Wang et al, 1999;Heresztyn and Nicholson, 2001;Bouaïcha et al, 2002). However, the lack of specificity makes necessary the use of other complementary analytical techniques if different variants have to be detected.…”

“…Radioisotopic methods have been used; however, they suffer from sophisticated labelling procedures and obvious hazardous problems (Craig et al, 1993;Jones and Orr, 1994;Sim and Mudge, 1993). Colorimetry is the most commonly used due to the simplicity, cost effectiveness, and sufficient sensitivity (An and Carmichael, 1994;Ward et al, 1997;Rivasseau et al, 1999;Wang et al, 1999;Heresztyn and Nicholson, 2001;Bouaïcha et al, 2002). Another approach for the detection of PP inhibition would be the development of a biosensor.…”

Development of Novel Method for the Detection of Microcystin Using Chemiluminescence Immunochromatography

“…For the quantitative analysis of MCLR in water sample, HPLC and ELISA (Enzyme Linked ImmunoSorbent Assay) are the most popular analytical tools today. However, using such analytical tools, it is impossible to obtain a quantitative analysis result with a low concentration of MCLR sample since the detection limits of HPLC and ELISA are 420 pg/mL 18 and 250 pg/mL 19 individually. The aim of this study was an application of the chemiluminescence immunochromatography on the detection of microcystins in water samples.…”

Liquid chromatographic determination of microcystins in water samples following pre-column excimer fluorescence derivatization with 4-(1-pyrene)butanoic acid hydrazide

“…Hence, in order to evaluate and control the quality of drinking water, it is necessary to determine MC content in environmental water sources, such as surface water and ground water used for drinking. Several analytical methods have been developed to quantify MCs such as enzymelinked immunosorbent assays (ELISA) [6], protein phosphatase inhibition assays (PPIA) [7], capillary electrophoresis (CE) [8], and liquid chromatography (LC) with UV [9,10] or mass spectrometry (MS) detection [11,12]. ELISA and PPIA have been used for fast screening analysis of MCs; they can determine total MC content but cannot identify individual MCs.…”