A combination of octanoate and oleate promotes in vitro differentiation of porcine intramuscular adipocytes

Sanosaka, Masato; Minashima, Takeshi; Suzuki, Keiichi; Watanabe, Kouichi; Ohwada, Shyuichi; Hagino, Akihiko; Rose, Michael T.; Yamaguchi, Takahiro; Aso, Hisashi

doi:10.1016/j.cbpb.2007.09.019

Cited by 34 publications

(35 citation statements)

References 38 publications

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“…However, rodent cells are prone to spontaneous immortalization during long-term culture [Todaro and Green, 1963;Aaronson and Todaro, 1968], and our established DFAT cell lines derived from mice have become immortalized and aneuploid [Yagi et al, 2004;Nobusue et al, 2008]. In addition, adipocyte differentiation in rodent preadipocyte cell lines does not always reflect that in other species [Tomlinson et al, 2006;Sanosaka et al, 2008], and the differentiation pattern of primary human preadipocytes and contribution of glucocorticoids to these events differ from the responses observed in rodent preadipocyte cell lines [Tomlinson et al, 2006].…”

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confidence: 61%

Establishment and characteristics of porcine preadipocyte cell lines derived from mature adipocytes

Nobusue

Kano

2009

J of Cellular Biochemistry

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Development of established preadipocyte cell lines, such as 3T3-L1 and 3T3-F442A, greatly facilitated the study of molecular mechanisms of adipocyte differentiation under defined conditions. Most of these cell lines are derived from mouse embryos, and preadipocyte cell lines of other species have not yet been maintained in culture long enough to study differentiation under a variety of conditions. This is the first report on the establishment of porcine preadipocyte cell lines derived from mature adipocytes by a simple method, known as ceiling culture, for culturing mature adipocytes in vitro. This cell line can proliferate extensively until the cells become confluent and fully differentiated into mature adipocytes, depending on adipogenic induction. No changes in their differentiation pattern are observed during their propagation, and they have been successfully carried and differentiated for at least 37 passages. This cell line maintains a normal phenotype without transforming spontaneously, even after long-term maintenance in culture. This achievement may lead to easy establishment of porcine preadipocyte cell lines and novel model systems for studying the mechanisms of adipocyte differentiation and metabolism as a substitute for human preadipocytes.

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confidence: 61%

Establishment and characteristics of porcine preadipocyte cell lines derived from mature adipocytes

Nobusue

Kano

2009

J of Cellular Biochemistry

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“…Neutral lipids (triacylglycerol or triglycerides) of muscle meat are rich in saturated and monounsaturated fatty acids (MUFA) and found in the i.m. adipocytes located in the perimysium (Sanosaka et al, 2008). Adipocyte number and size increase with the total lipid content of the muscle (Mourot & Hermier, 2001;Wood et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Effects of dietary turmeric supplementation on plasma lipoproteins, meat quality and fatty acid composition in broilers

Daneshyar¹,

Ghandkanlo²,

Bayeghra³

et al. 2011

SA J. An. Sci.

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An experiment with 200 day-old male broiler chickens was conducted to investigate the effect of the dietary supplementation of turmeric rhizome powder (TRP) on plasma lipoprotein concentrations, and the meat quality and fatty acid composition of the thigh muscle of the broilers. The four treatments were 0% (F.TRP), 0.25% (L.TRP), 0.50% (M.TRP) and 0.75% (H.TRP) TRP in the diets. The pH and the fat, protein, dry matter and ash concentrations of thigh meat did not show significant differences between treatments. There were no significant differences between treatments in the concentrations of plasma triglyceride, total cholesterol and very low-density lipoprotein-cholesterol (VLDL-c) at three weeks, and for plasma lowdensity lipoprotein (LDL-c) at three and six weeks of age. At week 6, the M.TRP-and H.TRP-fed birds showed lower plasma triglyceride and VLDL-c concentrations than the birds in the other treatments. At weeks 3 and 6 the concentration of plasma high-density lipoprotein-cholesterol (HDL-c) of the M.TRP-and H.TRP-fed birds was significantly higher than that of the F.TRP-fed birds. At week 6, the H.TRP-fed birds had significantly lower concentrations of saturated fatty acids (SFA) in the thigh and total cholesterol in the plasma than the F.TRP-fed birds and the other birds. Moreover, a significantly higher thigh vaccenic acid concentration was indicated for the H.TRP-fed birds compared with the L.TRP-and F.TRP-fed birds. In orthogonal comparisons, TRP consumption reduced the concentration of plasma triglycerides and dry matter of thigh meat, as well as triglyceride, palmitic acid and total SFA concentrations, but increased the thigh meat protein and plasma HDL-c concentrations significantly, compared with the control. In conclusion, supplementation of TRP in broiler chickens diets can decrease the concentrations of SFAs and triglycerides in thigh meat and improve the meat quality as a result.________________________________________________________________________________

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“…To induce differentiation, the preadipocytes were seeded in 6-well cell plates (Corning, Corning, NY, USA) at a density of 30.0×10 4 cells/well to obtain confluence. After reaching confluence, the medium was changed to adipogenic medium modified from previous report [27], which contained 50 nM insulin, 50 nM dexamethasone, 50 μM oleate and 0.5 mM octanoate and DMSO or 50 μM phloretin in DMEM/F12 supplemented with 5% FBS. Dexamethasone, insulin, oleate, octanoate, phloretin and DMSO were all purchased from Sigma-Aldrich (St. Louis, MO, USA).…”

Section: Cell Isolation and Culturementioning

confidence: 99%

Phloretin promotes adipocyte differentiation in vitro and improves glucose homeostasis in vivo

Shu

Zhu

et al. 2014

The Journal of Nutritional Biochemistry

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A combination of octanoate and oleate promotes in vitro differentiation of porcine intramuscular adipocytes

Cited by 34 publications

References 38 publications

Establishment and characteristics of porcine preadipocyte cell lines derived from mature adipocytes

Establishment and characteristics of porcine preadipocyte cell lines derived from mature adipocytes

Effects of dietary turmeric supplementation on plasma lipoproteins, meat quality and fatty acid composition in broilers

Phloretin promotes adipocyte differentiation in vitro and improves glucose homeostasis in vivo

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