Establishment and characteristics of porcine preadipocyte cell lines derived from mature adipocytes

Nobusue, Hiroyuki; Kano, Koichiro

doi:10.1002/jcb.22431

Cited by 45 publications

(38 citation statements)

References 52 publications

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“…This is specifically meaningful for Chinese domestic pig breeds which have high backfat thickness and low lean meat content. Because of physiological and anatomical similarities of pigs with human and the similarity adipocyte lipogenic patterns with that of human [46], miR-429 may also have a role in human obesity, and further studies on the function of miR-429 should also be explored in human precursor adipocytes.…”

Section: Discussionmentioning

confidence: 99%

miR-429 Inhibits Differentiation and Promotes Proliferation in Porcine Preadipocytes

Peng

Chen

et al. 2016

IJMS

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MicroRNAs (miRNAs) are crucial regulatory molecules for adipogenesis. They contribute to the controlling of proliferation and differentiation of preadipocytes. Previous studies revealed an important role of miR-429 in cell invasion, migration, and apoptosis. Our previous work has shown that the expression of miR-429 in subcutaneous fat can be observed in newly born (3-day-old) Rongchang piglets rather than their adult counterparts (180-day-old). This expression pattern suggests that miR-429 might be functionally related to postnatal adipogenesis. However, we currently lack a mechanistic understanding of miR-429 within the context of preadipocyte differentiation. In this study, we investigated the function of miR-429 in porcine subcutaneous and intramuscular preadipocyte proliferation and differentiation. In our porcine preadipocyte differentiation model, miR-429 expression decreased remarkably upon adipogenic induction. Overexpression of miR-429 notably down-regulated the expression of adipogenic marker genes: PPARγ, aP2, FAS and impaired the triglyceride accumulation, while the expression of lipolytic gene ATGL was not affected. In addition, we observed that miR-429 significantly promoted the proliferation of porcine preadipocytes. We also found that miR-429 could directly bind to the 3′-UTRs of KLF9 and p27, which have been well documented to promote preadipocyte differentiation and repress cell cycle progression. Taken together, our data support a novel role of miR-429 in regulating porcine preadipocyte differentiation and proliferation, and KLF9 and p27 are potent targets of miR-429 during these processes.

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Section: Discussionmentioning

confidence: 99%

miR-429 Inhibits Differentiation and Promotes Proliferation in Porcine Preadipocytes

Peng

Chen

et al. 2016

IJMS

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“…Also, time--lapse video images of cultured adipocytes pre--labeled with Hoechst 33342 showed that the adipocytes with a single nucleus divided asymmetrically and produced fibroblast--like cells. 23 Both the phenomena have proven to produce the multipotent and highly proliferative DFAT cells.…”

Section: Phenotypic Properties Of Dfat Cellsmentioning

confidence: 99%

Current challenges in dedifferentiated fat cells research

et al. 2016

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Dedifferentiated fat cells show great promises as a novel cell source for stem cell research. It has many advantages when used for cell-based therapeutics including abundance, pluripotency, and safety. However, there are many obstacles researchers need to overcome to make the next big move in DFAT cells research. In this review, we summarize the current main challenges in DFAT cells research including cell culture purity, phenotypic properties, and dedifferentiation mechanisms. The common methods to produce DFAT cells as well as the cell purity issue during DFAT cell production have been introduced. Current approaches to improve DFAT cell purity have been discussed. The phenotypic profile of DFAT cells have been listed and compared with other stem cells. Further studies on elucidating the underlying dedifferentiation mechanisms will dramatically advance DFAT cell research.

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“…Indeed, 96%-100% purity of isolated mature adipocyte samples utilizing fluorescence activated cell sorting (FACS), immunostaining or confocal microscopy was reported in several studies [1,[11][12][13][14][15][16]. It is not always clear from such work whether proliferative-competent DFAT cells are derived from dedifferentiated mature (lipid filled) adipocytes.…”

Section: Introductionmentioning

confidence: 95%